FastQCFastQC Report
Thu 26 May 2016
SRR1545963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545963_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences920637
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165071.792997674436287No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13800.1498962131654496No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTT207.855663E-444.00000415
AAGCGAC302.5271656E-644.00000415
GCGATAC207.855663E-444.0000049
TAATGCG207.855663E-444.0000041
TCACGAC750.044.025
ACCGGGT254.4421475E-544.04
GTTACAC254.4421475E-544.033
CGTTTTT122750.043.2830961
TACGGGA2250.043.0222244
ACGGTCT800.041.25000430
ATGGGAC4000.040.6999975
ATTTGCG1200.040.3333361
TACGTAG603.6379788E-1240.3333361
ACGGGAC1600.039.8750045
AGTACGG501.3478711E-939.62
GTTTTTT137350.039.515112
AAGGGAT8900.039.303375
GACACGA900.039.11110725
TTGTACG452.3501343E-839.1111071
CTCACGA850.038.82353224
Produced by FastQC (version 0.11.5)