##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545963_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	920637
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.109217856766566	31.0	28.0	33.0	25.0	33.0
2	28.61493618005794	31.0	28.0	33.0	16.0	33.0
3	28.074242073694627	31.0	26.0	33.0	16.0	34.0
4	32.01334728019838	35.0	32.0	35.0	25.0	37.0
5	33.599825990048195	35.0	33.0	35.0	28.0	37.0
6	33.51224749819961	35.0	33.0	37.0	28.0	37.0
7	34.292041271424026	35.0	35.0	37.0	31.0	37.0
8	34.73378758403149	35.0	35.0	37.0	32.0	37.0
9	36.59497825961807	39.0	37.0	39.0	32.0	39.0
10	36.400997352919774	39.0	35.0	39.0	32.0	39.0
11	36.39222733824515	38.0	35.0	39.0	32.0	39.0
12	35.15917674392839	37.0	34.0	39.0	30.0	39.0
13	35.058719125996454	37.0	34.0	39.0	27.0	39.0
14	35.79953010795786	38.0	34.0	40.0	27.0	41.0
15	36.414344633118155	38.0	35.0	40.0	31.0	41.0
16	36.06581312721518	37.0	34.0	40.0	31.0	41.0
17	35.649083189139695	37.0	34.0	40.0	30.0	41.0
18	35.301918128426294	37.0	33.0	39.0	29.0	40.0
19	34.67846936414678	36.0	33.0	39.0	27.0	40.0
20	35.157724488587796	35.0	34.0	39.0	30.0	40.0
21	35.25255773991269	35.0	34.0	39.0	30.0	40.0
22	35.611327808897535	35.0	34.0	39.0	31.0	41.0
23	35.98749887306289	36.0	35.0	40.0	31.0	41.0
24	35.569359041620096	35.0	34.0	40.0	31.0	41.0
25	35.221616120142905	35.0	34.0	39.0	30.0	41.0
26	35.32851384421873	35.0	34.0	39.0	30.0	41.0
27	35.65657799979797	35.0	34.0	40.0	31.0	41.0
28	35.609359606446404	36.0	35.0	40.0	31.0	41.0
29	35.75063461494596	36.0	35.0	40.0	31.0	41.0
30	35.420983514675164	36.0	35.0	40.0	30.0	41.0
31	34.69498836131939	35.0	34.0	39.0	28.0	41.0
32	34.60857428063395	35.0	34.0	40.0	27.0	41.0
33	34.27907633518966	35.0	34.0	40.0	24.0	41.0
34	33.83773843545284	35.0	34.0	40.0	21.0	41.0
35	33.499809371120215	35.0	33.0	40.0	18.0	41.0
36	33.080995006718176	35.0	33.0	40.0	16.0	41.0
37	32.9744731093797	35.0	33.0	40.0	15.0	41.0
38	32.862674430856025	35.0	33.0	40.0	15.0	41.0
39	32.59583527492378	35.0	32.0	39.0	15.0	40.0
40	32.44870453827078	35.0	32.0	39.0	15.0	40.0
41	32.140656958171355	35.0	31.0	39.0	12.0	40.0
42	32.350192312496674	35.0	32.0	39.0	12.0	40.0
43	32.147280632866156	35.0	32.0	39.0	10.0	40.0
44	31.87066129212708	35.0	31.0	39.0	10.0	40.0
45	31.857029426364573	35.0	31.0	39.0	10.0	40.0
46	31.70763069483412	35.0	31.0	38.0	10.0	40.0
47	31.733358533276416	35.0	31.0	38.0	10.0	40.0
48	31.763526775482628	35.0	31.0	38.0	10.0	40.0
49	31.833828099457225	35.0	31.0	38.0	10.0	40.0
50	31.889214750221857	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	19.0
14	29.0
15	99.0
16	274.0
17	567.0
18	1103.0
19	2032.0
20	2969.0
21	4431.0
22	6256.0
23	9093.0
24	13004.0
25	19627.0
26	27498.0
27	31657.0
28	29483.0
29	28154.0
30	30434.0
31	36516.0
32	48882.0
33	76486.0
34	123004.0
35	84166.0
36	80716.0
37	113173.0
38	123874.0
39	27084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.610351311103074	22.631178195097526	27.420688067066607	25.337782426732797
2	36.8620857080478	21.0439076422086	29.51260920427921	12.58139744546439
3	26.734424099835223	18.983486433849606	41.20158107918756	13.08050838712761
4	23.851203025731095	25.110874318542486	37.51163596509808	13.526286690628336
5	20.298228291932652	28.6617852638988	38.243194657612065	12.796791786556483
6	18.799266160278155	35.51464909622359	35.22941180943195	10.456672934066304
7	74.35992687671688	4.993933548184573	17.8486200315651	2.797519543533445
8	75.73354101562288	3.6154314892840502	17.553932766117374	3.0970947289756983
9	72.27962812704682	5.007076621947629	19.054415583992387	3.658879667013166
10	45.33469760611403	21.395294779592824	23.19089934469286	10.079108269600287
11	39.986444168548516	18.212933001823735	30.18692492263509	11.613697906992657
12	36.14073733730015	19.13164471990589	32.137965343561035	12.589652599232922
13	19.293380561502524	35.884284468254044	31.85859356076282	12.963741409480608
14	13.891794485774522	36.03168241120007	35.908289586449385	14.168233516576024
15	12.467997701591397	21.014471501797125	52.75010671958654	13.767424077024929
16	14.4150191660774	17.121188915935377	50.61799601797451	17.84579590001271
17	14.904354267751568	17.42532616003919	36.93507864663271	30.73524092557653
18	19.756212274761932	20.427378000232448	41.48768732953379	18.32872239547183
19	24.7711095687008	22.313680636342013	33.88523381093743	19.02997598401976
20	26.74821889626422	20.418253882909333	34.201536544805386	18.63199067602106
21	19.3928768885022	25.363959953814586	36.19363549368535	19.04952766399786
22	19.988877266501344	22.256654903072544	34.0071059494676	23.747361880958508
23	16.671826137772	26.943518455156596	33.92531475489254	22.459340652178874
24	17.085126928420213	21.15947979496805	45.481117965061145	16.27427531155059
25	15.590075132761338	22.568612819167598	42.176123705651634	19.665188342419434
26	14.630847988946783	30.123164721817613	35.95369293217631	19.292294357059298
27	15.307336116189116	29.75439831334174	36.98330612391203	17.954959446557112
28	13.879846236899015	24.889071371235353	44.803326392486944	16.42775599937869
29	14.752720127476953	21.0439076422086	43.661073799988486	20.54229843032596
30	17.07958728575975	26.758103356697593	38.29685315710753	17.865456200435133
31	25.1423742473961	23.7454067129607	32.92915666000823	18.183062379634972
32	26.034691197507815	23.19296313313499	33.599779283257135	17.17256638610006
33	24.4517654623918	24.694858016786203	31.74215244444879	19.111224076373208
34	18.205220950276818	24.69779076878292	34.32449488777879	22.772493393161476
35	19.05408972265942	24.05736462905575	35.833015618533686	21.05553002975114
36	27.218219558848926	23.569876074935074	30.371905539316803	18.8399988268992
37	18.786557568292388	29.61851413749393	33.23264218144611	18.362286112767574
38	20.510146778806412	29.435271447921384	29.3385992524741	20.7159825207981
39	20.257495625311606	28.43313922859933	31.33764990979072	19.971715236298344
40	22.9789808578191	24.871366238810737	29.816094725717086	22.33355817765308
41	17.667658371323334	22.91413445255839	31.927676163352114	27.490531012766162
42	21.515103129680863	23.630269041978543	28.389147948648596	26.465479879691994
43	20.88858040682701	25.496694136777037	29.035113731036226	24.579611725359722
44	19.649981480214244	28.32853774071648	30.30347465939344	21.718006119675834
45	16.99508058007662	34.15178838130555	27.032478599056958	21.82065243956087
46	20.931159621001548	29.58321249308902	29.246054633911083	20.239573251998344
47	20.14876656054449	25.89880702166	30.88720092718411	23.06522549061139
48	21.581687462050734	23.09857196701849	33.68363426627433	21.63610630465645
49	19.849843097768176	23.31277148322303	34.189479675485565	22.647905743523232
50	18.44168765756753	29.78046721997921	31.055997097661724	20.72184802479153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	503.0
1	757.0
2	1011.0
3	13353.0
4	25695.0
5	18588.5
6	11482.0
7	11918.5
8	12355.0
9	13074.5
10	13794.0
11	13585.0
12	13376.0
13	12710.0
14	12044.0
15	11172.5
16	10301.0
17	9589.0
18	8877.0
19	8143.0
20	7409.0
21	6960.5
22	6512.0
23	6689.0
24	6866.0
25	6706.0
26	6546.0
27	7626.5
28	8707.0
29	9793.0
30	10879.0
31	12817.0
32	14755.0
33	17218.0
34	19681.0
35	22373.5
36	25066.0
37	25701.0
38	26336.0
39	32933.5
40	39531.0
41	53104.0
42	66677.0
43	76471.5
44	86266.0
45	87118.0
46	87970.0
47	81532.0
48	75094.0
49	73206.5
50	71319.0
51	68196.0
52	65073.0
53	56178.0
54	47283.0
55	41311.0
56	35339.0
57	30722.5
58	26106.0
59	23905.5
60	21705.0
61	19118.0
62	16531.0
63	14944.5
64	13358.0
65	11078.5
66	8799.0
67	7487.5
68	6176.0
69	5091.0
70	4006.0
71	3385.5
72	2765.0
73	2420.5
74	2076.0
75	1603.0
76	1130.0
77	879.0
78	628.0
79	472.5
80	317.0
81	244.0
82	171.0
83	113.0
84	55.0
85	44.0
86	33.0
87	22.5
88	12.0
89	9.0
90	6.0
91	9.0
92	12.0
93	7.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	920637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.364677512383206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.74755115064936	21.769195382533038
2	7.232428692724994	4.102910150009035
3	3.225400544598922	2.744623388874408
4	1.9908215719410038	2.2587604749120955
5	1.4617800266961136	2.0731459525639093
6	1.0974596327532977	1.867745313954348
7	0.849046624355652	1.685805358497795
8	0.6727135932896439	1.526504330548581
9	0.5647026666919628	1.4415848127990314
>10	4.93007907851293	30.65912083999217
>50	1.0161430646840213	19.92523003045391
>100	0.21110150117825957	7.987343915116707
>500	0.0	0.0
>1k	3.859259619346609E-4	0.15106398326427448
>5k	0.0	0.0
>10k+	3.859259619346609E-4	1.8069660664807092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16507	1.792997674436287	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1380	0.1498962131654496	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.048770579500932505	0.0
2	0.0	0.0	0.0	0.17107719980839353	0.0
3	0.0	0.0	0.0	0.23103568507457337	0.0
4	0.0	0.0	0.0	0.3522561009388065	0.0
5	0.0	0.0	0.0	0.6070796633200708	0.0
6	0.0	0.0	0.0	0.9031789945439951	0.0
7	0.0	0.0	0.0	1.1087974956470357	0.0
8	1.0862044432278955E-4	0.0	0.0	1.6404945706070906	0.0
9	2.172408886455791E-4	0.0	0.0	1.9199749738496281	0.0
10	2.172408886455791E-4	0.0	0.0	2.3913877022105345	0.0
11	2.172408886455791E-4	0.0	0.0	2.6646767401266733	0.0
12	2.172408886455791E-4	0.0	0.0	2.96164503490518	0.0
13	2.172408886455791E-4	0.0	0.0	3.0686361725631275	0.0
14	2.172408886455791E-4	0.0	0.0	3.1082826347409456	0.0
15	2.172408886455791E-4	0.0	0.0	3.160746309348853	0.0
16	2.172408886455791E-4	0.0	0.0	3.264044351899826	0.0
17	2.172408886455791E-4	0.0	0.0	3.4013405935238317	0.0
18	2.172408886455791E-4	0.0	0.0	3.6142366643964996	0.0
19	2.172408886455791E-4	0.0	0.0	3.690922698088389	0.0
20	2.172408886455791E-4	0.0	0.0	3.7908535068653553	0.0
21	2.172408886455791E-4	0.0	0.0	3.8949118925265878	0.0
22	2.172408886455791E-4	0.0	0.0	3.9969064897456867	0.0
23	2.172408886455791E-4	0.0	0.0	4.1054183136241535	0.0
24	2.172408886455791E-4	0.0	0.0	4.199918100184981	0.0
25	2.172408886455791E-4	0.0	0.0	4.270304148106148	0.0
26	2.172408886455791E-4	0.0	0.0	4.341776400470544	0.0
27	3.2586133296836863E-4	0.0	0.0	4.417267609274883	0.0
28	3.2586133296836863E-4	0.0	0.0	4.490912270525734	0.0
29	3.2586133296836863E-4	0.0	0.0	4.571725881101889	0.0
30	3.2586133296836863E-4	0.0	0.0	4.701961793844914	0.0
31	3.2586133296836863E-4	0.0	0.0	4.799720193735425	0.0
32	4.344817772911582E-4	0.0	0.0	4.884661381195846	0.0
33	4.344817772911582E-4	0.0	0.0	4.973187043318919	0.0
34	4.344817772911582E-4	0.0	0.0	5.066492004992195	0.0
35	4.344817772911582E-4	0.0	0.0	5.1807607124197705	0.0
36	4.344817772911582E-4	0.0	0.0	5.270807060763363	0.0
37	4.344817772911582E-4	0.0	0.0	5.367044774433355	0.0
38	4.344817772911582E-4	0.0	0.0	5.4696910943183905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGTT	20	7.855663E-4	44.000004	15
AAGCGAC	30	2.5271656E-6	44.000004	15
GCGATAC	20	7.855663E-4	44.000004	9
TAATGCG	20	7.855663E-4	44.000004	1
TCACGAC	75	0.0	44.0	25
ACCGGGT	25	4.4421475E-5	44.0	4
GTTACAC	25	4.4421475E-5	44.0	33
CGTTTTT	12275	0.0	43.283096	1
TACGGGA	225	0.0	43.022224	4
ACGGTCT	80	0.0	41.250004	30
ATGGGAC	400	0.0	40.699997	5
ATTTGCG	120	0.0	40.333336	1
TACGTAG	60	3.6379788E-12	40.333336	1
ACGGGAC	160	0.0	39.875004	5
AGTACGG	50	1.3478711E-9	39.6	2
GTTTTTT	13735	0.0	39.51511	2
AAGGGAT	890	0.0	39.30337	5
GACACGA	90	0.0	39.111107	25
TTGTACG	45	2.3501343E-8	39.111107	1
CTCACGA	85	0.0	38.823532	24
>>END_MODULE