FastQCFastQC Report
Thu 26 May 2016
SRR1545962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545962_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences795641
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85411.073474091958559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG302.526729E-644.0000041
TGACGAT302.526729E-644.00000419
GCGATAA302.526729E-644.0000049
GCAACCG254.4416003E-544.012
CGACACG254.4416003E-544.024
ATGTACG207.855019E-444.01
ACGATGT207.855019E-444.036
ATATCCG254.4416003E-544.01
TACGATA254.4416003E-544.044
CCGAACT254.4416003E-544.012
CCTATAG254.4416003E-544.01
TCGACGG502.7284841E-1144.02
TCGAAAT207.855019E-444.032
ACGTTAG454.802132E-1043.9999961
ACGATTG850.043.9999961
CGTTTTT108350.043.390861
TACGGGA2100.041.9047624
GCGGAAT4450.041.528098
CGGGAAT14100.041.191499
GTTAGCG700.040.8571431
Produced by FastQC (version 0.11.5)