##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545962_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	795641
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.178479992861103	28.0	25.0	30.0	16.0	33.0
2	26.044406962436575	30.0	25.0	31.0	10.0	33.0
3	26.29342504973977	30.0	25.0	31.0	16.0	33.0
4	27.874225938582853	32.0	19.0	35.0	10.0	35.0
5	31.124694428768755	33.0	30.0	35.0	19.0	37.0
6	29.827897255169102	33.0	28.0	35.0	17.0	36.0
7	29.771224710642112	32.0	28.0	35.0	17.0	35.0
8	32.40502940396485	33.0	32.0	35.0	28.0	35.0
9	27.017830906149886	30.0	23.0	34.0	10.0	35.0
10	31.679281233621698	34.0	30.0	37.0	17.0	37.0
11	33.69933676117746	35.0	32.0	37.0	27.0	39.0
12	33.05154460365919	35.0	31.0	37.0	23.0	39.0
13	32.85084479055252	35.0	31.0	37.0	22.0	39.0
14	32.639779749912336	35.0	30.0	38.0	21.0	40.0
15	31.808466381194535	34.0	27.0	38.0	18.0	40.0
16	30.499233950990458	33.0	27.0	38.0	17.0	40.0
17	30.569543299050704	32.0	26.0	37.0	17.0	40.0
18	31.49484378004653	34.0	28.0	38.0	18.0	39.0
19	31.081429941393164	34.0	27.0	37.0	18.0	39.0
20	31.80316876581272	34.0	30.0	37.0	21.0	39.0
21	32.54548722350909	34.0	31.0	37.0	23.0	39.0
22	32.90676825352138	34.0	31.0	37.0	25.0	39.0
23	34.22026894038894	35.0	33.0	38.0	28.0	40.0
24	33.860096450534854	35.0	33.0	38.0	27.0	40.0
25	32.61179979412826	35.0	31.0	37.0	23.0	39.0
26	26.24880567994862	33.0	20.0	36.0	0.0	39.0
27	29.486738365669943	33.0	23.0	37.0	12.0	39.0
28	31.01723013268547	34.0	27.0	36.0	18.0	39.0
29	32.356075164552855	34.0	30.0	37.0	23.0	39.0
30	32.10517557541655	34.0	30.0	37.0	22.0	39.0
31	31.014603319838972	33.0	29.0	36.0	19.0	38.0
32	31.204299426500143	34.0	30.0	37.0	18.0	39.0
33	30.943464451932467	34.0	29.0	37.0	18.0	39.0
34	30.92274153795493	34.0	29.0	37.0	15.0	39.0
35	30.095615987612504	34.0	27.0	37.0	10.0	39.0
36	29.730746655841013	33.0	26.0	37.0	10.0	39.0
37	29.718209594528187	33.0	26.0	37.0	10.0	39.0
38	29.504723864154812	33.0	26.0	36.0	10.0	38.0
39	29.351399689055743	33.0	26.0	36.0	10.0	39.0
40	29.297291114962654	33.0	26.0	36.0	10.0	38.0
41	28.74709322420539	33.0	24.0	36.0	9.0	38.0
42	28.951034448953738	33.0	24.0	36.0	9.0	38.0
43	28.505040589914294	33.0	24.0	35.0	8.0	38.0
44	28.202925691360804	32.0	23.0	35.0	8.0	38.0
45	27.69782100218566	31.0	23.0	35.0	8.0	38.0
46	27.31206285246738	31.0	23.0	35.0	8.0	38.0
47	27.472269528593927	31.0	23.0	34.0	8.0	38.0
48	27.553758541854933	31.0	23.0	34.0	8.0	37.0
49	27.42925766771697	31.0	23.0	34.0	8.0	37.0
50	29.17081824591744	33.0	25.0	36.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	16.0
12	35.0
13	107.0
14	290.0
15	620.0
16	1263.0
17	2391.0
18	3902.0
19	6084.0
20	8907.0
21	12446.0
22	16636.0
23	21801.0
24	28294.0
25	35207.0
26	41143.0
27	45515.0
28	49909.0
29	57695.0
30	68523.0
31	79202.0
32	85053.0
33	81727.0
34	67761.0
35	50671.0
36	25317.0
37	4975.0
38	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.797844756617618	23.417219575160154	27.28780945175022	26.497126216472
2	36.717816201025336	18.305617734631575	31.429627181103037	13.546938883240053
3	27.986994134289205	14.640522547229216	43.074954659199314	14.297528659282262
4	25.13935304993081	24.750609885614242	36.721586745781075	13.388450318673875
5	19.691544302015608	28.765360256698685	39.135615183229625	12.407480258056083
6	18.767509467209457	34.139894751527386	36.406243519376204	10.686352261886958
7	77.34443046549889	3.2890461904301063	17.568978974185594	1.7975443698854132
8	79.353376711356	1.9786562029860202	16.85772854842825	1.8102385372297307
9	67.19613494025572	2.916139314087635	17.07893384076487	12.808791904891779
10	46.97922806894064	21.074831488070625	23.003465130630524	8.942475312358212
11	41.52262138326205	17.40609144073772	30.3039938866901	10.767293289310128
12	37.202959626263606	18.949626778911593	32.366481868078694	11.480931726746107
13	18.761979334901042	37.002618014908734	31.91401649739015	12.32138615280007
14	13.106287886119494	36.4392986284015	36.687274788503856	13.767138696975142
15	11.031231422211777	19.377332239037457	53.363137394880354	16.228298943870413
16	12.715785134250247	15.13433822540568	50.91354015190268	21.236336488441395
17	14.072552822189907	16.38050326717703	37.69501571688739	31.85192819374567
18	19.345156923788494	19.82954624007561	42.68118410187509	18.144112734260805
19	25.643097829297385	21.26285598655675	34.59072621948844	18.50331996465743
20	27.546971561294605	19.875923940571187	34.72143843768735	17.855666060446858
21	19.149842705441273	25.043329843484685	36.86247943482048	18.944348016253564
22	19.60909505669014	21.92584343944065	34.144042350758696	24.32101915311051
23	16.022175830556744	26.680877430901624	34.718799056358336	22.578147682183296
24	16.13893703315943	20.32047116727268	47.88089100486275	15.65970079470515
25	14.853558325928404	21.365791858388395	44.178970163679345	19.601679652003856
26	11.435810874502446	41.878309438553316	29.993803738118068	16.692075948826165
27	14.2682440950127	30.84003966613083	37.74189615668373	17.149820082172738
28	13.082659138983537	24.738921196871452	46.433881612435755	15.74453805170925
29	13.855997868385364	20.66748696962575	45.125502582194734	20.351012579794155
30	16.609375333850316	26.793742403923375	39.07666900021492	17.52021326201138
31	25.95806400122668	23.312901170251408	33.60309486313551	17.1259399653864
32	26.62821548914649	22.247847961580664	34.29863468574395	16.825301863528903
33	24.84122864457714	24.041873156360722	32.14150603098634	18.975392168075803
34	17.725833636024287	23.922346887603833	34.897271508129926	23.45454796824196
35	18.757328996368965	23.238872808213753	36.37620484615549	21.62759334926179
36	27.472817514431757	23.185079703031892	30.344213030751305	18.99788975178504
37	17.941508796052492	30.363945548306333	32.91094853080724	18.78359712483394
38	19.491077005835546	30.440738976498196	29.25892456522477	20.809259452441488
39	19.6758336988667	28.87772249041967	31.10724560448745	20.339198206226175
40	22.893867963063744	24.31410648772499	29.686378655700246	23.105646893511018
41	17.019861972924975	22.152453179260494	31.880584333889278	28.94710051392525
42	20.75144443285351	22.742543433533466	27.62954649144526	28.87646564216776
43	20.250464719641144	24.57905009922817	29.181502712906955	25.988982468223732
44	18.67701639307175	27.652546814455263	30.73220208611673	22.93823470635626
45	16.076723044689754	35.447519672817265	26.300052410572107	22.17570487192088
46	19.803780850911405	29.57238251925177	28.877471120769293	21.74636550906753
47	19.386004491975655	25.18723896832868	30.443504042652403	24.983252497043264
48	20.71838932382821	22.496075491333404	34.597136145573195	22.188399039265196
49	19.375069912184014	23.186462236108998	33.98756474339557	23.45090310831141
50	17.870873924295	30.627129572256834	30.763120553113776	20.738875950334386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	297.0
1	453.0
2	609.0
3	7699.0
4	14789.0
5	12007.0
6	9225.0
7	9466.0
8	9707.0
9	10597.5
10	11488.0
11	11888.0
12	12288.0
13	12166.5
14	12045.0
15	11743.0
16	11441.0
17	10809.5
18	10178.0
19	9481.5
20	8785.0
21	8293.0
22	7801.0
23	7749.0
24	7697.0
25	7274.5
26	6852.0
27	7713.0
28	8574.0
29	9620.0
30	10666.0
31	12127.0
32	13588.0
33	15590.0
34	17592.0
35	19318.0
36	21044.0
37	21499.5
38	21955.0
39	27187.5
40	32420.0
41	43939.0
42	55458.0
43	64069.0
44	72680.0
45	73851.5
46	75023.0
47	69640.5
48	64258.0
49	61593.0
50	58928.0
51	56227.5
52	53527.0
53	47496.0
54	41465.0
55	36228.5
56	30992.0
57	27468.0
58	23944.0
59	21581.5
60	19219.0
61	16971.0
62	14723.0
63	13372.0
64	12021.0
65	10031.0
66	8041.0
67	6861.0
68	5681.0
69	4742.5
70	3804.0
71	3261.0
72	2718.0
73	2277.5
74	1837.0
75	1474.0
76	1111.0
77	857.0
78	603.0
79	454.5
80	306.0
81	225.0
82	144.0
83	103.5
84	63.0
85	42.0
86	21.0
87	16.5
88	12.0
89	10.0
90	8.0
91	9.0
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	795641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.49885373825258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.20478421208936	35.758138850262895
2	7.059495584290731	6.141599317738048
3	2.7177124823667875	3.546521333192887
4	1.4896282488978192	2.5918848529270218
5	1.0009663146191448	2.1770443658267946
6	0.7451983898441517	1.9449165459487248
7	0.5331002347442106	1.6232474397276573
8	0.42197783862920935	1.4684441826652739
9	0.32193107438796437	1.2603269446740517
>10	3.0783094793601378	28.528596958187286
>50	0.3815086740764798	11.009983342653024
>100	0.04475267694998865	2.690298173350913
>500	3.173948719857153E-4	0.07980048979763701
>1k	0.0	0.0
>5k	3.173948719857153E-4	1.1791972030477815
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8541	1.073474091958559	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2568482519126087E-4	0.0	0.0	0.034940381403170524	0.0
2	1.2568482519126087E-4	0.0	0.0	0.1413954283401685	0.0
3	1.2568482519126087E-4	0.0	0.0	0.18613922610825737	0.0
4	1.2568482519126087E-4	0.0	0.0	0.27198196171388855	0.0
5	1.2568482519126087E-4	0.0	0.0	0.4479407169816538	0.0
6	1.2568482519126087E-4	0.0	0.0	0.6203802971440637	0.0
7	1.2568482519126087E-4	0.0	0.0	0.745311013384177	0.0
8	1.2568482519126087E-4	0.0	0.0	1.074856625035663	0.0
9	1.2568482519126087E-4	0.0	0.0	1.294050960169222	0.0
10	1.2568482519126087E-4	0.0	0.0	1.6047438480420189	0.0
11	1.2568482519126087E-4	0.0	0.0	1.7976700547106044	0.0
12	1.2568482519126087E-4	0.0	0.0	1.9975089267647093	0.0
13	1.2568482519126087E-4	0.0	0.0	2.070657495026023	0.0
14	1.2568482519126087E-4	0.0	0.0	2.093783502861215	0.0
15	1.2568482519126087E-4	0.0	0.0	2.126084502935369	0.0
16	1.2568482519126087E-4	0.0	0.0	2.1867902735027482	0.0
17	1.2568482519126087E-4	0.0	0.0	2.287463818480948	0.0
18	1.2568482519126087E-4	0.0	0.0	2.427728083394395	0.0
19	1.2568482519126087E-4	0.0	0.0	2.476242425918222	0.0
20	1.2568482519126087E-4	0.0	0.0	2.5383307295627047	0.0
21	1.2568482519126087E-4	0.0	0.0	2.5974025974025974	0.0
22	1.2568482519126087E-4	0.0	0.0	2.661753227900523	0.0
23	1.2568482519126087E-4	0.0	0.0	2.725852488748066	0.0
24	1.2568482519126087E-4	0.0	0.0	2.775623679523805	0.0
25	1.2568482519126087E-4	0.0	0.0	2.812575018130036	0.0
26	1.2568482519126087E-4	0.0	0.0	2.8571931310729335	0.0
27	1.2568482519126087E-4	0.0	0.0	2.9158879444372525	0.0
28	1.2568482519126087E-4	0.0	0.0	2.9713149523465985	0.0
29	1.2568482519126087E-4	0.0	0.0	3.0362940069704805	0.0
30	1.2568482519126087E-4	0.0	0.0	3.13495659474562	0.0
31	1.2568482519126087E-4	0.0	0.0	3.2025750306985183	0.0
32	1.2568482519126087E-4	0.0	0.0	3.267302715672018	0.0
33	1.2568482519126087E-4	0.0	0.0	3.3359266302264463	0.0
34	1.2568482519126087E-4	0.0	0.0	3.4029166420533885	0.0
35	1.2568482519126087E-4	0.0	0.0	3.488130953533063	0.0
36	1.2568482519126087E-4	0.0	0.0	3.5636675334730112	0.0
37	1.2568482519126087E-4	0.0	0.0	3.6286465880968928	0.0
38	1.2568482519126087E-4	0.0	0.0	3.708205082442961	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	30	2.526729E-6	44.000004	1
TGACGAT	30	2.526729E-6	44.000004	19
GCGATAA	30	2.526729E-6	44.000004	9
GCAACCG	25	4.4416003E-5	44.0	12
CGACACG	25	4.4416003E-5	44.0	24
ATGTACG	20	7.855019E-4	44.0	1
ACGATGT	20	7.855019E-4	44.0	36
ATATCCG	25	4.4416003E-5	44.0	1
TACGATA	25	4.4416003E-5	44.0	44
CCGAACT	25	4.4416003E-5	44.0	12
CCTATAG	25	4.4416003E-5	44.0	1
TCGACGG	50	2.7284841E-11	44.0	2
TCGAAAT	20	7.855019E-4	44.0	32
ACGTTAG	45	4.802132E-10	43.999996	1
ACGATTG	85	0.0	43.999996	1
CGTTTTT	10835	0.0	43.39086	1
TACGGGA	210	0.0	41.904762	4
GCGGAAT	445	0.0	41.52809	8
CGGGAAT	1410	0.0	41.19149	9
GTTAGCG	70	0.0	40.857143	1
>>END_MODULE