FastQCFastQC Report
Thu 26 May 2016
SRR1545961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545961_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences494817
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97821.9768924673161998No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.852132E-444.01
ACTGCGC207.852132E-444.034
CTCGTAG254.439153E-544.01
ATAGGTT207.852132E-444.015
ACGCATG254.439153E-544.01
CCGGATA302.5247828E-644.020
TCGATAG207.852132E-444.01
ATCGTAA207.852132E-444.014
GTATGCG207.852132E-444.01
AGACCGT207.852132E-444.014
CGGGCAC351.443841E-744.06
CGTAAAT207.852132E-444.016
CGGTCTA551.8189894E-1244.031
ATACGAG351.443841E-744.01
TCGACGG551.8189894E-1244.02
ACCTTCG207.852132E-444.033
TGCGTAG454.783942E-1044.01
CGTTTTT70600.043.220961
CGTATGG850.041.4117662
AACGGGA800.041.254
Produced by FastQC (version 0.11.5)