##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545961_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	494817
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.19377871010899	31.0	28.0	33.0	25.0	33.0
2	28.89404769844205	31.0	28.0	33.0	16.0	33.0
3	28.32609429344586	31.0	28.0	33.0	16.0	34.0
4	32.157359185314974	35.0	32.0	35.0	25.0	37.0
5	33.656830707109904	35.0	33.0	35.0	28.0	37.0
6	33.49641180476823	35.0	33.0	37.0	28.0	37.0
7	34.323172000961975	35.0	35.0	37.0	31.0	37.0
8	34.78798626562951	35.0	35.0	37.0	32.0	37.0
9	36.594324770571745	39.0	37.0	39.0	32.0	39.0
10	36.36916678287125	39.0	35.0	39.0	32.0	39.0
11	36.33385878011467	38.0	35.0	39.0	32.0	39.0
12	35.04234292677899	37.0	34.0	39.0	29.0	39.0
13	35.01525008235368	37.0	34.0	39.0	27.0	39.0
14	35.7494184718795	38.0	33.0	40.0	27.0	41.0
15	36.403943275999005	38.0	35.0	40.0	31.0	41.0
16	36.06638817987256	37.0	34.0	40.0	31.0	41.0
17	35.651242782685316	37.0	34.0	40.0	29.0	41.0
18	35.312388216249644	37.0	33.0	39.0	29.0	40.0
19	34.72168296562163	36.0	33.0	39.0	27.0	40.0
20	35.10049169693038	35.0	34.0	39.0	29.0	40.0
21	35.249154738014255	35.0	34.0	39.0	30.0	40.0
22	35.59813223878727	35.0	34.0	39.0	31.0	40.0
23	35.93435350846879	36.0	35.0	40.0	31.0	41.0
24	35.59390441314668	35.0	34.0	40.0	31.0	41.0
25	35.25346946446868	35.0	34.0	39.0	30.0	41.0
26	35.343516896145445	35.0	34.0	40.0	30.0	41.0
27	35.61933199546499	35.0	34.0	40.0	31.0	41.0
28	35.5424308380674	36.0	35.0	40.0	31.0	41.0
29	35.68747435920755	36.0	35.0	40.0	31.0	41.0
30	35.32502925323907	36.0	34.0	40.0	30.0	41.0
31	34.60284307127686	35.0	34.0	39.0	27.0	41.0
32	34.45499043080573	35.0	34.0	39.0	25.0	41.0
33	34.08451003098115	35.0	33.0	40.0	23.0	41.0
34	33.64870447054164	35.0	33.0	40.0	21.0	41.0
35	33.32674503907505	35.0	33.0	40.0	18.0	41.0
36	32.87428685756552	35.0	33.0	40.0	15.0	41.0
37	32.76595387789829	35.0	33.0	40.0	15.0	41.0
38	32.58542451047559	35.0	32.0	39.0	15.0	40.0
39	32.42457312501389	35.0	32.0	39.0	13.0	40.0
40	32.243126246673015	35.0	31.0	39.0	12.0	40.0
41	31.946244773320238	35.0	31.0	39.0	10.0	40.0
42	32.076755649058136	35.0	31.0	39.0	10.0	40.0
43	31.84622597849306	35.0	31.0	39.0	10.0	40.0
44	31.653724508252544	35.0	31.0	38.0	10.0	40.0
45	31.581362402666038	35.0	31.0	38.0	10.0	40.0
46	31.426418251596044	35.0	30.0	38.0	10.0	40.0
47	31.36929410266826	35.0	30.0	38.0	10.0	40.0
48	31.40333497030215	35.0	31.0	38.0	10.0	40.0
49	31.40625524183688	35.0	31.0	38.0	10.0	40.0
50	31.50903263226607	35.0	31.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	5.0
14	20.0
15	57.0
16	157.0
17	324.0
18	623.0
19	1084.0
20	1700.0
21	2549.0
22	3597.0
23	5126.0
24	7326.0
25	10851.0
26	15095.0
27	17258.0
28	16455.0
29	15465.0
30	16642.0
31	19950.0
32	26680.0
33	40756.0
34	65840.0
35	45821.0
36	44424.0
37	60095.0
38	63755.0
39	13155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.74628600068308	22.4531493461219	27.16822582894282	24.632338824252198
2	39.17993925026828	19.595123045489547	28.8668335970672	12.358104107174976
3	27.39901822289857	18.30636376680672	41.80838572644028	12.486232283854436
4	24.481171827160345	24.471067081365433	37.76871045255115	13.279050638923076
5	20.014065806146515	28.845815725813782	38.39338583759249	12.746732630447216
6	18.460966377468843	35.375300363568755	36.16367263048764	10.000060628474769
7	75.1316143139787	4.549358651784397	18.07051899995352	2.2485080342833816
8	76.35307598566743	3.2183615356788473	17.633993981613404	2.79456849704032
9	73.05084505989082	4.601297045170235	18.742484595315034	3.6053732996239014
10	46.676852250427935	20.330950634274895	23.102076121071022	9.890120994226148
11	40.095025029455336	18.8475739515821	29.490700602444946	11.566700416517621
12	35.711788398539255	18.9936885757765	32.238180984080984	13.056342041603259
13	19.549045404664756	33.33333333333333	33.42447814040342	13.693143121598489
14	14.870346006705509	34.44465327585754	36.5157219739823	14.16927874345465
15	13.888973095103847	19.724665886580294	52.29226158357534	14.09409943474052
16	15.905274071020195	16.436379510000666	50.248475699096836	17.4098707198823
17	16.855726460489436	17.16472958689778	36.28634424443784	29.693199708174937
18	21.326874379821227	19.793782347817476	40.72071088907616	18.158632383285134
19	26.23050541917517	21.72055527599092	33.67467164628539	18.37426765854851
20	27.53118829789599	21.482891654894637	33.06535547485232	17.920564572357055
21	21.096890365529074	24.6658865802913	36.17539413560973	18.061828918569894
22	20.832752310450125	21.13185278597946	34.42868777750158	23.60670712606883
23	18.39629600438142	25.84409993997781	33.71751576845581	22.04208828718496
24	18.530082838706026	21.392959417319936	43.544381054005825	16.53257668996821
25	17.099250834146765	21.769866435470085	41.298500253629115	19.83238247675403
26	15.96327531188298	29.04447502814172	35.05114011846804	19.941109541507267
27	17.219497309106195	27.972361499301762	36.82573557497014	17.982405616621904
28	15.345269059066279	24.198238944902865	43.83782287189001	16.618669124140844
29	16.531768310304617	21.19268335566482	42.715791898823205	19.55975643520736
30	19.01511063686171	25.520748074540688	38.021127002507995	17.443014286089607
31	26.447151169018042	22.604316343213753	32.99583482378334	17.952697663984868
32	27.887885824456315	22.80358193028938	32.4827158323178	16.8258164129365
33	26.46048943346732	23.528698488532125	31.621791490591473	18.38902058740908
34	19.06846369465883	24.556553230790374	33.826040738293145	22.548942336257646
35	19.41606695000374	24.53452488495747	35.41915496031866	20.630253204720127
36	28.064112591119546	23.001837042785514	30.572110497416215	18.36193986867872
37	19.94616191440472	29.39551389705689	32.36994686924661	18.28837731929178
38	20.215958627128817	29.561433823009313	28.863600078412826	21.35900747144904
39	20.203630837259027	28.698488532123996	30.14043575705766	20.957444873559318
40	23.35813037951404	24.36011697253732	29.230806540599858	23.05094610734878
41	18.568682967642584	22.719106255443933	30.68871926388948	28.023491513024005
42	21.94649739196511	23.99634612392056	28.026927126594277	26.030229357520053
43	21.852725350988344	24.373253142070705	28.939183576958754	24.834837929982196
44	20.11026298611406	27.49339654862303	30.559984802462324	21.836355662800592
45	18.053745121933968	32.3034576419161	27.1482184322689	22.49457880388103
46	21.569994563646762	28.449709690653314	29.325184866324317	20.655110879375606
47	20.810319774785427	24.644666614121988	30.77723683705289	23.767776774039696
48	22.202349555492233	22.8686564932086	32.90428582688145	22.024708124417714
49	20.748680825436473	22.4644666614122	33.735300121054856	23.051552392096472
50	19.541365797860625	28.090384930186314	30.866360694963994	21.50188857698907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	445.5
2	635.0
3	7801.0
4	14967.0
5	10546.0
6	6125.0
7	6379.0
8	6633.0
9	7016.0
10	7399.0
11	7360.5
12	7322.0
13	7056.5
14	6791.0
15	6412.0
16	6033.0
17	5522.0
18	5011.0
19	4702.5
20	4394.0
21	4123.5
22	3853.0
23	3594.0
24	3335.0
25	3424.0
26	3513.0
27	3664.0
28	3815.0
29	4403.5
30	4992.0
31	5654.5
32	6317.0
33	7364.0
34	8411.0
35	9238.5
36	10066.0
37	10991.5
38	11917.0
39	15677.5
40	19438.0
41	26452.0
42	33466.0
43	39349.0
44	45232.0
45	46549.5
46	47867.0
47	43556.0
48	39245.0
49	36997.0
50	34749.0
51	31402.5
52	28056.0
53	26040.5
54	24025.0
55	22049.0
56	20073.0
57	19040.5
58	18008.0
59	16929.5
60	15851.0
61	14270.0
62	12689.0
63	11696.0
64	10703.0
65	9052.5
66	7402.0
67	6438.0
68	5474.0
69	4591.0
70	3708.0
71	3256.5
72	2805.0
73	2373.0
74	1941.0
75	1500.5
76	1060.0
77	826.0
78	592.0
79	443.0
80	294.0
81	243.5
82	193.0
83	137.0
84	81.0
85	53.5
86	26.0
87	33.0
88	40.0
89	24.0
90	8.0
91	6.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	494817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.25700389759305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80282670096085	26.725805564443473
2	8.030492072699513	5.662621806135137
3	3.7117996063975713	3.926007995695306
4	2.228326872505073	3.1425651691609082
5	1.456434878324591	2.5674765090840284
6	1.1006059864536808	2.3282441732467003
7	0.8176404126607426	2.017928585120657
8	0.6541227065056446	1.8449925450218903
9	0.5280100770933038	1.6754448011442318
>10	5.283350395529306	38.75327158881392
>50	0.3605543873197711	8.062649015348757
>100	0.025261772359983947	1.31290556384605
>500	0.0	0.0
>1k	0.0	0.0
>5k	5.741311899996351E-4	1.980086682938955
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9782	1.9768924673161998	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04385459674990956	0.0
2	0.0	0.0	0.0	0.17056810901808142	0.0
3	0.0	0.0	0.0	0.22493164139469743	0.0
4	0.0	0.0	0.0	0.3498262994197855	0.0
5	0.0	0.0	0.0	0.6347801308362485	0.0
6	0.0	0.0	0.0	0.9183192978414242	0.0
7	0.0	0.0	0.0	1.1139471764308826	0.0
8	0.0	0.0	0.0	1.7099250834146766	0.0
9	0.0	0.0	0.0	2.007408799616828	0.0
10	0.0	0.0	0.0	2.4849590858842765	0.0
11	0.0	0.0	0.0	2.7915370733018467	0.0
12	0.0	0.0	0.0	3.112665894664088	0.0
13	0.0	0.0	0.0	3.24382549508202	0.0
14	0.0	0.0	0.0	3.290307325738606	0.0
15	0.0	0.0	0.0	3.348106471685492	0.0
16	4.041898317964015E-4	0.0	0.0	3.4592586754295023	0.0
17	4.041898317964015E-4	0.0	0.0	3.591832940258722	0.0
18	4.041898317964015E-4	0.0	0.0	3.801203273129258	0.0
19	4.041898317964015E-4	0.0	0.0	3.8986130225921904	0.0
20	4.041898317964015E-4	0.0	0.0	4.0071379924295245	0.0
21	4.041898317964015E-4	0.0	0.0	4.127182372473055	0.0
22	4.041898317964015E-4	0.0	0.0	4.247024657600689	0.0
23	4.041898317964015E-4	0.0	0.0	4.372121410541675	0.0
24	4.041898317964015E-4	0.0	0.0	4.473977248154368	0.0
25	6.062847476946022E-4	0.0	0.0	4.552996360270565	0.0
26	6.062847476946022E-4	0.0	0.0	4.637472035116013	0.0
27	6.062847476946022E-4	0.0	0.0	4.729425221849694	0.0
28	6.062847476946022E-4	0.0	0.0	4.8139008966951415	0.0
29	8.08379663592803E-4	0.0	0.0	4.9179797783827155	0.0
30	8.08379663592803E-4	0.0	0.0	5.063892307661217	0.0
31	8.08379663592803E-4	0.0	0.0	5.177671745311903	0.0
32	8.08379663592803E-4	0.0	0.0	5.273262640531752	0.0
33	8.08379663592803E-4	0.0	0.0	5.373097528985463	0.0
34	8.08379663592803E-4	0.0	0.0	5.480005739495612	0.0
35	8.08379663592803E-4	0.0	0.0	5.609144390754562	0.0
36	8.08379663592803E-4	0.0	0.0	5.717467265675998	0.0
37	8.08379663592803E-4	0.0	0.0	5.832459272822073	0.0
38	8.08379663592803E-4	0.0	0.0	5.962406303744617	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.852132E-4	44.0	1
ACTGCGC	20	7.852132E-4	44.0	34
CTCGTAG	25	4.439153E-5	44.0	1
ATAGGTT	20	7.852132E-4	44.0	15
ACGCATG	25	4.439153E-5	44.0	1
CCGGATA	30	2.5247828E-6	44.0	20
TCGATAG	20	7.852132E-4	44.0	1
ATCGTAA	20	7.852132E-4	44.0	14
GTATGCG	20	7.852132E-4	44.0	1
AGACCGT	20	7.852132E-4	44.0	14
CGGGCAC	35	1.443841E-7	44.0	6
CGTAAAT	20	7.852132E-4	44.0	16
CGGTCTA	55	1.8189894E-12	44.0	31
ATACGAG	35	1.443841E-7	44.0	1
TCGACGG	55	1.8189894E-12	44.0	2
ACCTTCG	20	7.852132E-4	44.0	33
TGCGTAG	45	4.783942E-10	44.0	1
CGTTTTT	7060	0.0	43.22096	1
CGTATGG	85	0.0	41.411766	2
AACGGGA	80	0.0	41.25	4
>>END_MODULE