FastQCFastQC Report
Thu 26 May 2016
SRR1545960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545960_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences498185
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101492.0371950179150318No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGT207.852181E-444.00000439
GGTCGGT207.852181E-444.00000436
TACGCGG207.852181E-444.0000042
ACTCGCT207.852181E-444.00000434
ACGTAAG207.852181E-444.0000041
GTCCAAC207.852181E-444.00000426
ATCATCG207.852181E-444.00000432
GTCGGTT207.852181E-444.00000437
TCACGAC600.044.025
CTCACGA600.044.024
CAACCCG254.439196E-544.023
TGACGTA302.5248173E-644.031
GTTACGG254.439196E-544.02
ACTAACC600.044.014
CGGTCTA600.044.031
AGGGCTC1150.044.06
TATCGGG254.439196E-544.03
GTACGAA254.439196E-544.020
CGTTTTT68900.043.2656061
GGCACCG850.041.4117628
Produced by FastQC (version 0.11.5)