##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545960_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	498185
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.167584331121972	31.0	28.0	33.0	25.0	33.0
2	28.862394492005983	31.0	28.0	33.0	16.0	33.0
3	28.32580266366912	31.0	28.0	33.0	16.0	34.0
4	32.136262633359095	35.0	32.0	35.0	25.0	37.0
5	33.70298383130765	35.0	33.0	35.0	30.0	37.0
6	33.58169154029126	35.0	33.0	37.0	28.0	37.0
7	34.38473057197628	35.0	35.0	37.0	31.0	37.0
8	34.82071921073497	35.0	35.0	37.0	32.0	37.0
9	36.69407147947048	39.0	37.0	39.0	32.0	39.0
10	36.369983038429496	39.0	35.0	39.0	32.0	39.0
11	36.37976253801299	39.0	35.0	39.0	32.0	39.0
12	34.99128837680781	37.0	34.0	39.0	29.0	39.0
13	35.02350130975441	37.0	34.0	39.0	27.0	39.0
14	35.771307847486376	38.0	33.0	40.0	27.0	41.0
15	36.44913435771852	38.0	35.0	40.0	31.0	41.0
16	36.09530596063711	37.0	34.0	40.0	31.0	41.0
17	35.694230055100014	37.0	34.0	40.0	30.0	41.0
18	35.374258558567604	37.0	34.0	39.0	29.0	40.0
19	34.788696970001105	36.0	33.0	39.0	27.0	40.0
20	35.06681252948202	35.0	34.0	39.0	29.0	40.0
21	35.15163443299176	35.0	34.0	39.0	30.0	40.0
22	35.56518160924155	35.0	34.0	39.0	31.0	40.0
23	35.94154781858145	36.0	35.0	40.0	31.0	41.0
24	35.469249375232096	35.0	34.0	40.0	31.0	41.0
25	35.15336672119795	35.0	34.0	39.0	30.0	41.0
26	35.29942089785923	35.0	34.0	39.0	30.0	41.0
27	35.626441984403385	35.0	34.0	40.0	31.0	41.0
28	35.54154380400855	36.0	35.0	40.0	31.0	41.0
29	35.70101869787328	36.0	35.0	40.0	31.0	41.0
30	35.35466342824453	36.0	34.0	40.0	30.0	41.0
31	34.62286299266337	35.0	34.0	39.0	28.0	41.0
32	34.52203699428927	35.0	34.0	39.0	26.0	41.0
33	34.171677188193144	35.0	33.0	40.0	23.0	41.0
34	33.66465268926202	35.0	33.0	40.0	21.0	41.0
35	33.345700894246114	35.0	33.0	40.0	18.0	41.0
36	32.88504872687857	35.0	33.0	40.0	15.0	41.0
37	32.76678944568785	35.0	33.0	40.0	15.0	41.0
38	32.607683892529884	35.0	32.0	40.0	15.0	40.0
39	32.42478396579584	35.0	32.0	39.0	13.0	40.0
40	32.22728705199876	35.0	31.0	39.0	12.0	40.0
41	31.916679546754718	35.0	31.0	39.0	10.0	40.0
42	32.05506588917771	35.0	31.0	39.0	10.0	40.0
43	31.836632977709083	35.0	31.0	39.0	10.0	40.0
44	31.64427672450997	35.0	31.0	38.0	10.0	40.0
45	31.57059927536959	35.0	30.0	38.0	10.0	40.0
46	31.401858747252525	35.0	30.0	38.0	10.0	40.0
47	31.350534440017263	35.0	30.0	38.0	10.0	40.0
48	31.379852865903228	35.0	31.0	38.0	10.0	40.0
49	31.43784939329767	35.0	31.0	38.0	10.0	40.0
50	31.56213655569718	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	6.0
13	6.0
14	23.0
15	64.0
16	139.0
17	362.0
18	633.0
19	1049.0
20	1709.0
21	2444.0
22	3598.0
23	5055.0
24	7280.0
25	10698.0
26	15211.0
27	17561.0
28	16462.0
29	15742.0
30	16655.0
31	20201.0
32	26305.0
33	41095.0
34	66594.0
35	46390.0
36	45237.0
37	61869.0
38	63759.0
39	12035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.768539799472084	22.272448989833094	27.199735038188628	24.759276172506198
2	39.33759547156177	19.56421811174564	28.735911358230375	12.362275058462217
3	27.311540893443198	18.177986089504902	41.95750574585747	12.552967271194435
4	24.306833806718387	24.596886698716343	37.83253209149212	13.263747403073156
5	19.882975199975913	28.86939590714293	38.47907905697683	12.768549835904333
6	18.32772965866094	35.383843351365456	36.368618083643625	9.919808906329978
7	74.82722281883237	4.628802553268365	18.25285787408292	2.2911167538163535
8	76.10405772955829	3.257223722111264	17.860232644499533	2.7784859038309064
9	72.82274657004928	4.626393809528588	19.012415066692093	3.5384445537300397
10	46.5973483745998	20.10036432249064	23.26143902365587	10.04084827925369
11	39.965675401708204	18.766321747945042	29.62373415498259	11.644268695364172
12	35.63736362997682	18.646085289601253	32.43313227014061	13.283418810281322
13	19.571042885675	33.16318235193753	33.486355470357395	13.779419292030068
14	14.889850156066522	34.29288316589219	36.59002177905798	14.22724489898331
15	13.919527886227003	19.677830524805042	52.34822405331353	14.054417535654427
16	15.834077702058472	16.405552154320183	50.31143049268846	17.44893965093289
17	16.728123086805102	17.1442335678513	36.50571574816584	29.621927597177756
18	21.193733251703684	19.769362786916506	40.819775786103556	18.217128175276255
19	26.168190531629815	21.643164687816775	33.79888997059325	18.389754809960156
20	27.487178457801818	21.195941266798478	33.241867980770195	18.075012294629506
21	21.1678392565011	24.498328934030532	36.31522426407861	18.018607545389763
22	20.879994379597942	21.042183124742817	34.58674990214479	23.49107259351446
23	18.4600098357036	25.66897839156137	33.835221855334865	22.035789917400162
24	18.57843973624256	21.24953581500848	43.61833455443259	16.553689894316367
25	17.176751608338268	21.65801860754539	41.442436042835496	19.72279374128085
26	15.97619358270522	28.92479701315776	35.153808324216904	19.94520107992011
27	17.350381886247078	27.782651023214267	36.90355992251874	17.963407168019913
28	15.324226943806016	24.10951754870179	43.941708401497436	16.62454710599476
29	16.504712104940936	21.377600690506537	42.685949998494536	19.431737206057992
30	19.155333861918766	25.352027861135923	37.99953832411654	17.493099952828768
31	26.353061613657573	22.556480022481608	33.154952477493296	17.935505886367515
32	27.601995242731114	22.817627989602258	32.77617752441362	16.80419924325301
33	26.30870058311671	23.538645282375022	31.762497867258148	18.39015626725012
34	19.026666800485764	24.508164637634614	33.88741130303	22.577757258849623
35	19.468069090799602	24.5940764976866	35.390266668004855	20.547587743508938
36	28.081335246946416	23.082790529622528	30.457962403524796	18.37791181990626
37	19.96547467306322	29.340305308269016	32.44617963206439	18.24804038660337
38	20.214980378774953	29.461946867127676	28.986822164456978	21.336250589640397
39	20.237261258367877	28.501661029537217	30.213474913937592	21.04760279815731
40	23.326073647339843	24.393749309995282	29.336290735369392	22.943886307295482
41	18.525848831257466	22.762628340877384	30.76909180324578	27.942431024619367
42	21.912542529381657	23.995102221062456	28.138944368056045	25.953410881499845
43	21.83827293073858	24.366249485632846	29.125927115429008	24.669550468199564
44	20.066842638778766	27.516484839969085	30.64363640013248	21.773036121119667
45	18.106727420536547	32.38194646567039	27.155574736292742	22.355751377500326
46	21.577124963617933	28.43762859178819	29.331673976534823	20.653572468059053
47	20.955468350110902	24.726356674729267	30.716099440970723	23.602075534189108
48	22.170880295472564	22.93224404588657	32.93134076698415	21.965534891656713
49	20.726236237542278	22.54604213294258	33.72903640213977	22.998685227375372
50	19.61219225789616	28.112648915563494	30.82549655248552	21.449662274054816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	258.0
1	472.5
2	687.0
3	8132.5
4	15578.0
5	10913.0
6	6248.0
7	6489.5
8	6731.0
9	7092.0
10	7453.0
11	7505.5
12	7558.0
13	7144.5
14	6731.0
15	6427.0
16	6123.0
17	5568.0
18	5013.0
19	4645.0
20	4277.0
21	4116.0
22	3955.0
23	3641.0
24	3327.0
25	3415.5
26	3504.0
27	3636.0
28	3768.0
29	4333.0
30	4898.0
31	5616.5
32	6335.0
33	7422.5
34	8510.0
35	9247.0
36	9984.0
37	11086.0
38	12188.0
39	15902.5
40	19617.0
41	26652.5
42	33688.0
43	39592.0
44	45496.0
45	46810.5
46	48125.0
47	43708.5
48	39292.0
49	37018.5
50	34745.0
51	31444.5
52	28144.0
53	26024.5
54	23905.0
55	22152.0
56	20399.0
57	19144.5
58	17890.0
59	16915.5
60	15941.0
61	14502.0
62	13063.0
63	11912.0
64	10761.0
65	9056.5
66	7352.0
67	6470.5
68	5589.0
69	4723.0
70	3857.0
71	3348.5
72	2840.0
73	2388.5
74	1937.0
75	1521.0
76	1105.0
77	872.0
78	639.0
79	474.0
80	309.0
81	261.5
82	214.0
83	143.0
84	72.0
85	50.5
86	29.0
87	31.0
88	33.0
89	20.5
90	8.0
91	5.0
92	2.0
93	2.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	498185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.23047648409062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4574918232073	26.58403391225966
2	8.244013348011059	5.808810367832656
3	3.7211589260559683	3.9329460611393614
4	2.246846644624227	3.16629911507167
5	1.5687836632005638	2.7634497977506505
6	1.1413542227973776	2.4126271863768323
7	0.8121742294893733	2.0029299565106853
8	0.6594030011918732	1.8584865541623257
9	0.5187060600263632	1.6446835484922682
>10	5.250248062686882	38.48976746743409
>50	0.35007507748481537	7.82661486591259
>100	0.029172923123734595	1.4640769627122312
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.720181004653842E-4	2.0452742043450014
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10149	2.0371950179150318	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04235374409105051	0.0
2	0.0	0.0	0.0	0.15857562953521281	0.0
3	2.0072864498128207E-4	0.0	0.0	0.2123709063901964	0.0
4	2.0072864498128207E-4	0.0	0.0	0.32919497776930257	0.0
5	4.0145728996256413E-4	0.0	0.0	0.5995764625590895	0.0
6	4.0145728996256413E-4	0.0	0.0	0.8884249826871544	0.0
7	4.0145728996256413E-4	0.0	0.0	1.0797193813543162	0.0
8	4.0145728996256413E-4	0.0	0.0	1.6499894617461386	0.0
9	4.0145728996256413E-4	0.0	0.0	1.9398416250991097	0.0
10	4.0145728996256413E-4	0.0	0.0	2.408944468420366	0.0
11	6.021859349438462E-4	0.0	0.0	2.719270953561428	0.0
12	6.021859349438462E-4	0.0	0.0	3.017553720003613	0.0
13	8.029145799251283E-4	0.0	0.0	3.137187992412457	0.0
14	8.029145799251283E-4	0.0	0.0	3.1873701536577776	0.0
15	8.029145799251283E-4	0.0	0.0	3.2439756315424995	0.0
16	8.029145799251283E-4	0.0	0.0	3.361201160211568	0.0
17	8.029145799251283E-4	0.0	0.0	3.4754157592059176	0.0
18	8.029145799251283E-4	0.0	0.0	3.686381565081245	0.0
19	8.029145799251283E-4	0.0	0.0	3.776709455322822	0.0
20	0.0010036432249064104	0.0	0.0	3.8847014663227517	0.0
21	0.0010036432249064104	0.0	0.0	3.996105864287363	0.0
22	0.0010036432249064104	0.0	0.0	4.112127021086544	0.0
23	0.0010036432249064104	0.0	0.0	4.2498268715437035	0.0
24	0.0012043718698876923	0.0	0.0	4.354607224223933	0.0
25	0.0012043718698876923	0.0	0.0	4.431887752541726	0.0
26	0.0012043718698876923	0.0	0.0	4.51539086885394	0.0
27	0.0012043718698876923	0.0	0.0	4.6047151158706106	0.0
28	0.0012043718698876923	0.0	0.0	4.6886196894727865	0.0
29	0.0012043718698876923	0.0	0.0	4.781757780744101	0.0
30	0.0012043718698876923	0.0	0.0	4.925479490550699	0.0
31	0.0012043718698876923	0.0	0.0	5.037887531740217	0.0
32	0.0014051005148689743	0.0	0.0	5.138051125585877	0.0
33	0.0016058291598502565	0.0	0.0	5.239619819946405	0.0
34	0.0016058291598502565	0.0	0.0	5.345002358561579	0.0
35	0.0016058291598502565	0.0	0.0	5.480694922568925	0.0
36	0.0016058291598502565	0.0	0.0	5.592099320533537	0.0
37	0.0016058291598502565	0.0	0.0	5.711131407007437	0.0
38	0.0016058291598502565	0.0	0.0	5.844816684564971	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTAGT	20	7.852181E-4	44.000004	39
GGTCGGT	20	7.852181E-4	44.000004	36
TACGCGG	20	7.852181E-4	44.000004	2
ACTCGCT	20	7.852181E-4	44.000004	34
ACGTAAG	20	7.852181E-4	44.000004	1
GTCCAAC	20	7.852181E-4	44.000004	26
ATCATCG	20	7.852181E-4	44.000004	32
GTCGGTT	20	7.852181E-4	44.000004	37
TCACGAC	60	0.0	44.0	25
CTCACGA	60	0.0	44.0	24
CAACCCG	25	4.439196E-5	44.0	23
TGACGTA	30	2.5248173E-6	44.0	31
GTTACGG	25	4.439196E-5	44.0	2
ACTAACC	60	0.0	44.0	14
CGGTCTA	60	0.0	44.0	31
AGGGCTC	115	0.0	44.0	6
TATCGGG	25	4.439196E-5	44.0	3
GTACGAA	25	4.439196E-5	44.0	20
CGTTTTT	6890	0.0	43.265606	1
GGCACCG	85	0.0	41.411762	8
>>END_MODULE