FastQCFastQC Report
Thu 26 May 2016
SRR1545959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545959_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences489535
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98842.019058902836365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA302.5247264E-644.00000431
CGGGTAT254.4390832E-544.06
TTAGCAC254.4390832E-544.029
CAACCCG207.852049E-444.023
ATTCGAT207.852049E-444.014
ACGGGTA207.852049E-444.05
TAGCCGT408.296411E-944.044
TCTAACG207.852049E-444.01
AGACACG254.4390832E-544.024
ACGTAAG207.852049E-444.01
TCGACGG351.4438046E-744.02
TGTAACG207.852049E-444.01
CGTTTTT66300.043.4690781
AACGGGA650.040.6153834
ACCTGGT2200.040.011
ATTGGGC557.8216544E-1140.04
GTTTTTT74050.039.6029742
CTGTTAG452.3461325E-839.111111
TACCTGG2250.039.1111110
TACGGGA850.038.823534
Produced by FastQC (version 0.11.5)