##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545959_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	489535
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.054153431317474	31.0	28.0	33.0	25.0	33.0
2	28.762535875882214	31.0	28.0	33.0	16.0	33.0
3	28.201801709785816	31.0	27.0	33.0	16.0	34.0
4	32.05101371709888	35.0	32.0	35.0	25.0	37.0
5	33.643042887638266	35.0	33.0	35.0	28.0	37.0
6	33.52330681156608	35.0	33.0	37.0	28.0	37.0
7	34.30620691063969	35.0	35.0	37.0	31.0	37.0
8	34.78156822290541	35.0	35.0	37.0	32.0	37.0
9	36.67021357002053	39.0	37.0	39.0	32.0	39.0
10	36.44161704474654	39.0	35.0	39.0	32.0	39.0
11	36.38437496808196	39.0	35.0	39.0	32.0	39.0
12	35.149194643896756	37.0	34.0	39.0	30.0	39.0
13	35.08514406528644	37.0	34.0	39.0	27.0	39.0
14	35.8134290704444	38.0	34.0	40.0	27.0	41.0
15	36.403006935152746	38.0	35.0	40.0	31.0	41.0
16	36.05176953639678	37.0	34.0	40.0	31.0	41.0
17	35.6418397050262	37.0	34.0	40.0	30.0	41.0
18	35.30513446433861	37.0	33.0	39.0	29.0	40.0
19	34.67426231015147	36.0	33.0	39.0	27.0	40.0
20	35.13980818531872	35.0	34.0	39.0	30.0	40.0
21	35.20838142318731	35.0	34.0	39.0	30.0	40.0
22	35.57161592123137	35.0	34.0	39.0	31.0	40.0
23	35.9190599242138	36.0	35.0	40.0	31.0	41.0
24	35.4788074397132	35.0	34.0	40.0	31.0	41.0
25	35.15903050854382	35.0	34.0	39.0	30.0	41.0
26	35.28677009815437	35.0	34.0	39.0	30.0	41.0
27	35.62601856864167	35.0	34.0	40.0	31.0	41.0
28	35.55039782650883	36.0	35.0	40.0	31.0	41.0
29	35.679718508380404	36.0	35.0	40.0	31.0	41.0
30	35.340047187637246	36.0	34.0	40.0	30.0	41.0
31	34.597781568222906	35.0	34.0	39.0	27.0	41.0
32	34.500193040334196	35.0	34.0	40.0	26.0	41.0
33	34.16952005474583	35.0	33.0	40.0	23.0	41.0
34	33.67750007660331	35.0	33.0	40.0	21.0	41.0
35	33.339779586750694	35.0	33.0	40.0	18.0	41.0
36	32.913342253362885	35.0	33.0	40.0	15.0	41.0
37	32.80478617463511	35.0	33.0	40.0	15.0	41.0
38	32.626860183643664	35.0	32.0	40.0	15.0	41.0
39	32.39767943047995	35.0	32.0	39.0	13.0	40.0
40	32.24077542974455	35.0	31.0	39.0	12.0	40.0
41	31.924620302940546	35.0	31.0	39.0	10.0	40.0
42	32.097584442378995	35.0	31.0	39.0	10.0	40.0
43	31.88148753408847	35.0	31.0	39.0	10.0	40.0
44	31.69844444217472	35.0	31.0	39.0	10.0	40.0
45	31.629824220944364	35.0	31.0	38.0	10.0	40.0
46	31.45985476012951	35.0	30.0	38.0	10.0	40.0
47	31.42714821207881	35.0	31.0	38.0	10.0	40.0
48	31.441631344030558	35.0	31.0	38.0	10.0	40.0
49	31.514149141532272	35.0	31.0	38.0	10.0	40.0
50	31.56136742010275	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	8.0
13	6.0
14	22.0
15	55.0
16	145.0
17	354.0
18	625.0
19	1090.0
20	1642.0
21	2522.0
22	3482.0
23	4925.0
24	7190.0
25	10762.0
26	15106.0
27	17126.0
28	16193.0
29	15573.0
30	16658.0
31	19875.0
32	25873.0
33	40107.0
34	65037.0
35	45321.0
36	43697.0
37	59417.0
38	63236.0
39	13484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.72482049291675	22.44129633223365	27.06813608832872	24.765747086520882
2	39.369605850449915	19.342437210822517	28.87352283289244	12.414434105835129
3	27.248102791424518	18.301040783600765	41.86278815610733	12.588068268867394
4	24.58046922079116	24.525110564106754	37.59588180620385	13.298538408898239
5	19.924009519237643	29.022439662128345	38.29021418284699	12.763336635787024
6	18.35680799125701	35.36764480578508	36.27728354458823	9.998263658369677
7	74.89147864810484	4.650127161489985	18.158047943456545	2.3003462469486347
8	76.12959236826785	3.3072201170498534	17.720898403587075	2.842289111095223
9	72.89938410940995	4.67280174042714	18.85503590141665	3.572778248746259
10	46.73863972953926	20.32561512455698	23.090892377460243	9.844852768443522
11	40.1981472213427	18.562104854606922	29.617289877128293	11.62245804692208
12	35.67896064632764	19.0325512986814	32.33047688112188	12.95801117386908
13	19.55508799166556	33.379022950350844	33.384742663956615	13.681146394026985
14	14.886371760956827	34.44922222108736	36.499330997783616	14.165075020172205
15	13.924642773243997	19.733420490873993	52.31536049516377	14.026576240718233
16	15.795193397816295	16.413739569183	50.418458332907754	17.372608700092947
17	16.771834496001308	17.08846149917779	36.352661198892825	29.787042805928078
18	21.28938686712901	19.762631885360598	40.72660790341855	18.221373344091845
19	26.300673087726107	21.645234763602193	33.712196267886874	18.341895880784826
20	27.64235447925072	21.292246723931896	33.07812515959022	17.98727363722716
21	21.258745544240966	24.52286353376163	36.188832259184736	18.02955866281267
22	20.97582399624133	21.029752724524293	34.493550001532064	23.500873277702308
23	18.552912457740508	25.741366807276293	33.76387796582471	21.941842769158487
24	18.48917850613337	21.36047473622928	43.61567610078952	16.534670656847826
25	17.083967438487544	21.77454114618975	41.3898904062018	19.7516010091209
26	15.912856077706394	29.093936082200457	35.04529808900283	19.94790975109032
27	17.428988734206953	27.87808839000276	36.7285280929862	17.96439478280409
28	15.359065235376429	24.10369023665315	43.893490761641154	16.643753766329272
29	16.414556671126682	21.241790678909577	42.855362742194124	19.48828990776962
30	19.114057217563605	25.475604400093964	38.027311632467544	17.38302674987488
31	26.534364243618946	22.575505326483295	32.95310856220699	17.937021867690767
32	27.833556334072128	22.619833106928002	32.61339842912152	16.933212129878353
33	26.436107734891273	23.508227195195442	31.69252453859275	18.36314053132054
34	19.079330384957153	24.537162817776053	33.7667378226276	22.616768974639196
35	19.41822341609895	24.556773264424404	35.546385855965354	20.47861746351129
36	28.18674864922835	23.015106172183806	30.53040129919209	18.267743879395752
37	19.938104527766146	29.302297077839174	32.46366449794193	18.295933896452755
38	20.044327780444707	29.624643794621424	29.007527551656164	21.323500873277702
39	20.23675528818164	28.665161837253724	30.154125854126875	20.94395702043776
40	23.29843627115528	24.48159988560573	29.37624480374233	22.843719039496666
41	18.516959972218533	22.772018343938637	30.764501006056765	27.94652067778606
42	21.96880713329997	24.00645510535508	27.95121901396223	26.073518747382717
43	21.828878425444554	24.297139121819686	29.072282880692903	24.801699572042857
44	19.96649882030907	27.541238113720162	30.82292379502998	21.66933927094079
45	18.111268857180793	32.35294718457312	27.177014922324243	22.358769035921846
46	21.64400911068667	28.64595994157721	29.286567865423308	20.423463082312807
47	20.90371474971146	24.661158037729685	30.781455871388154	23.653671341170703
48	22.253362885186963	22.888251095427293	32.837897188147934	22.02048883123781
49	20.703524773509553	22.468464971861053	33.73773070362691	23.09027955100248
50	19.511168762192693	28.163665519319352	30.946101913039925	21.379063805448027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	413.0
2	575.0
3	7918.5
4	15262.0
5	10725.5
6	6189.0
7	6411.0
8	6633.0
9	7036.5
10	7440.0
11	7338.0
12	7236.0
13	6872.0
14	6508.0
15	6302.0
16	6096.0
17	5531.0
18	4966.0
19	4577.0
20	4188.0
21	4039.5
22	3891.0
23	3563.0
24	3235.0
25	3256.0
26	3277.0
27	3478.5
28	3680.0
29	4281.0
30	4882.0
31	5513.0
32	6144.0
33	7245.5
34	8347.0
35	9183.5
36	10020.0
37	10913.0
38	11806.0
39	15575.0
40	19344.0
41	26303.5
42	33263.0
43	38996.5
44	44730.0
45	46039.5
46	47349.0
47	43000.0
48	38651.0
49	36503.5
50	34356.0
51	30922.5
52	27489.0
53	25571.5
54	23654.0
55	21833.0
56	20012.0
57	18761.5
58	17511.0
59	16596.5
60	15682.0
61	14165.5
62	12649.0
63	11577.5
64	10506.0
65	8856.0
66	7206.0
67	6340.5
68	5475.0
69	4671.0
70	3867.0
71	3326.5
72	2786.0
73	2418.0
74	2050.0
75	1584.0
76	1118.0
77	868.5
78	619.0
79	434.0
80	249.0
81	228.0
82	207.0
83	133.5
84	60.0
85	45.5
86	31.0
87	30.0
88	29.0
89	18.5
90	8.0
91	7.0
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	489535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.868858010580716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22329745897186	26.22950478184567
2	8.126777597960315	5.667429082936929
3	3.8484286189947654	4.025709332387511
4	2.2873209870376847	3.1902508288655347
5	1.5971326850989689	2.7845096410386736
6	1.0900257251329837	2.2804771342525383
7	0.8795790970193427	2.146894305012958
8	0.6638382574142541	1.8517825551815164
9	0.5593079739479082	1.7552187294997672
>10	5.331933812396252	38.59230625363396
>50	0.36357705022436027	8.030459184820973
>100	0.028193373846153943	1.4211516522181262
>500	0.0	0.0
>1k	0.0	0.0
>5k	5.873619551282071E-4	2.024306518305802
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9884	2.019058902836365	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03676958746565619	0.0
2	0.0	0.0	0.0	0.15953915450376377	0.0
3	0.0	0.0	0.0	0.21796194347697304	0.0
4	0.0	0.0	0.0	0.3348075214233916	0.0
5	0.0	0.0	0.0	0.6030212344367614	0.0
6	0.0	0.0	0.0	0.8818572727179875	0.0
7	0.0	0.0	0.0	1.0734676785112403	0.0
8	0.0	0.0	0.0	1.6442133861725923	0.0
9	0.0	0.0	0.0	1.9330589232639137	0.0
10	0.0	0.0	0.0	2.395947174359341	0.0
11	0.0	0.0	0.0	2.7033817806694107	0.0
12	0.0	0.0	0.0	2.99733420490874	0.0
13	2.0427548592031214E-4	0.0	0.0	3.119286670003166	0.0
14	2.0427548592031214E-4	0.0	0.0	3.1646358278774755	0.0
15	2.0427548592031214E-4	0.0	0.0	3.2175431787308364	0.0
16	2.0427548592031214E-4	0.0	0.0	3.322540778493877	0.0
17	2.0427548592031214E-4	0.0	0.0	3.4393863564402953	0.0
18	2.0427548592031214E-4	0.0	0.0	3.6405977100718028	0.0
19	2.0427548592031214E-4	0.0	0.0	3.7259848631864934	0.0
20	6.128264577609364E-4	0.0	0.0	3.829143983576251	0.0
21	6.128264577609364E-4	0.0	0.0	3.9349586852829725	0.0
22	6.128264577609364E-4	0.0	0.0	4.057932527807001	0.0
23	6.128264577609364E-4	0.0	0.0	4.1764123096407815	0.0
24	6.128264577609364E-4	0.0	0.0	4.2681320028190015	0.0
25	6.128264577609364E-4	0.0	0.0	4.350455023644887	0.0
26	6.128264577609364E-4	0.0	0.0	4.428488259266446	0.0
27	6.128264577609364E-4	0.0	0.0	4.517143820155862	0.0
28	6.128264577609364E-4	0.0	0.0	4.601918146812792	0.0
29	6.128264577609364E-4	0.0	0.0	4.70548581817439	0.0
30	6.128264577609364E-4	0.0	0.0	4.83642640464931	0.0
31	6.128264577609364E-4	0.0	0.0	4.941219728926431	0.0
32	6.128264577609364E-4	0.0	0.0	5.030283840787686	0.0
33	6.128264577609364E-4	0.0	0.0	5.121390707508145	0.0
34	6.128264577609364E-4	0.0	0.0	5.226388307271186	0.0
35	6.128264577609364E-4	0.0	0.0	5.355286138886903	0.0
36	6.128264577609364E-4	0.0	0.0	5.455381126987856	0.0
37	6.128264577609364E-4	0.0	0.0	5.57631221465268	0.0
38	6.128264577609364E-4	0.0	0.0	5.70684425015576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	30	2.5247264E-6	44.000004	31
CGGGTAT	25	4.4390832E-5	44.0	6
TTAGCAC	25	4.4390832E-5	44.0	29
CAACCCG	20	7.852049E-4	44.0	23
ATTCGAT	20	7.852049E-4	44.0	14
ACGGGTA	20	7.852049E-4	44.0	5
TAGCCGT	40	8.296411E-9	44.0	44
TCTAACG	20	7.852049E-4	44.0	1
AGACACG	25	4.4390832E-5	44.0	24
ACGTAAG	20	7.852049E-4	44.0	1
TCGACGG	35	1.4438046E-7	44.0	2
TGTAACG	20	7.852049E-4	44.0	1
CGTTTTT	6630	0.0	43.469078	1
AACGGGA	65	0.0	40.615383	4
ACCTGGT	220	0.0	40.0	11
ATTGGGC	55	7.8216544E-11	40.0	4
GTTTTTT	7405	0.0	39.602974	2
CTGTTAG	45	2.3461325E-8	39.11111	1
TACCTGG	225	0.0	39.11111	10
TACGGGA	85	0.0	38.82353	4
>>END_MODULE