FastQCFastQC Report
Thu 26 May 2016
SRR1545958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545958_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences425627
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50591.1885994074624027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTG207.850887E-444.0000041
CAACCCG207.850887E-444.00000423
TTCGTAG207.850887E-444.0000041
TCGATTA302.523946E-644.00000441
CGACAGG408.290954E-944.0000042
TTATCGG207.850887E-444.0000042
ACGGGTG207.850887E-444.0000045
ATGTACG207.850887E-444.0000041
CGAATAA207.850887E-444.00000413
CGTCGGG408.290954E-944.0000043
GCGTAAA207.850887E-444.0000041
TACGTAG302.523946E-644.0000041
TAGTACA207.850887E-444.00000441
GTCATTA254.438102E-544.020
CGGGTAT351.4432044E-744.06
TTGGCGA254.438102E-544.05
TGATTCG351.4432044E-744.015
ACTAACC351.4432044E-744.014
CGTTTTT61450.043.1407661
CGGGAAT9200.041.3695649
Produced by FastQC (version 0.11.5)