##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545958_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	425627
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.09697458102987	28.0	25.0	30.0	16.0	33.0
2	26.224104673810636	30.0	25.0	31.0	10.0	33.0
3	26.48886701266602	30.0	25.0	31.0	16.0	33.0
4	27.980346641542948	32.0	19.0	35.0	10.0	37.0
5	31.20696760308909	33.0	30.0	35.0	19.0	37.0
6	29.871941864590358	33.0	28.0	35.0	17.0	37.0
7	29.82836380210842	32.0	28.0	35.0	17.0	35.0
8	32.44623813808804	33.0	32.0	35.0	28.0	35.0
9	27.085213109130766	31.0	23.0	34.0	10.0	35.0
10	31.717719975471482	34.0	30.0	37.0	17.0	38.0
11	33.698245177115176	35.0	32.0	37.0	27.0	39.0
12	33.01520815173849	35.0	31.0	37.0	23.0	39.0
13	32.871861982440024	35.0	31.0	37.0	22.0	39.0
14	32.681227929619126	35.0	30.0	38.0	21.0	40.0
15	31.767871869030866	34.0	27.0	38.0	18.0	40.0
16	30.434899571690707	33.0	26.0	38.0	17.0	40.0
17	30.54054606498178	32.0	26.0	37.0	17.0	40.0
18	31.49290106125786	34.0	28.0	38.0	18.0	39.0
19	31.103795106983345	34.0	27.0	37.0	18.0	39.0
20	31.800120763015503	34.0	30.0	37.0	21.0	39.0
21	32.50925810627615	34.0	31.0	37.0	23.0	39.0
22	32.886717712927044	34.0	31.0	37.0	25.0	39.0
23	34.16197985560127	35.0	33.0	38.0	28.0	40.0
24	33.80451193180884	35.0	33.0	38.0	26.0	40.0
25	32.5744912799235	35.0	31.0	37.0	23.0	39.0
26	26.28865415023013	33.0	20.0	36.0	0.0	39.0
27	29.464554645264517	33.0	23.0	37.0	12.0	39.0
28	30.970297936925995	34.0	27.0	36.0	18.0	39.0
29	32.29110465266536	34.0	30.0	37.0	23.0	39.0
30	32.05193514509183	34.0	30.0	37.0	22.0	39.0
31	30.933441722447117	33.0	29.0	36.0	18.0	38.0
32	31.10434723361065	34.0	29.0	37.0	18.0	39.0
33	30.810211758182636	34.0	29.0	37.0	17.0	39.0
34	30.728776604867644	34.0	29.0	37.0	15.0	39.0
35	29.85862738970977	34.0	27.0	37.0	10.0	39.0
36	29.54878802331619	33.0	25.0	37.0	10.0	39.0
37	29.576370859931348	33.0	26.0	37.0	10.0	39.0
38	29.333362310191788	33.0	26.0	36.0	10.0	38.0
39	29.220540520220755	33.0	25.0	36.0	9.0	39.0
40	29.17004795278495	33.0	25.0	36.0	10.0	38.0
41	28.549814743895478	33.0	23.0	35.0	8.0	38.0
42	28.706463640699486	33.0	24.0	36.0	8.0	38.0
43	28.287777796051472	32.0	23.0	35.0	8.0	38.0
44	28.018262469251244	32.0	23.0	35.0	8.0	38.0
45	27.505503645210478	31.0	23.0	35.0	8.0	38.0
46	27.084717369903696	31.0	23.0	34.0	7.0	38.0
47	27.207780521442483	31.0	23.0	34.0	8.0	37.0
48	27.314063722461217	31.0	23.0	34.0	8.0	37.0
49	27.15136962645697	31.0	23.0	34.0	8.0	37.0
50	28.871702218139358	33.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	34.0
13	62.0
14	154.0
15	357.0
16	683.0
17	1349.0
18	2107.0
19	3386.0
20	5082.0
21	6832.0
22	9182.0
23	12083.0
24	15375.0
25	19313.0
26	22517.0
27	24331.0
28	26930.0
29	30950.0
30	36684.0
31	42150.0
32	44624.0
33	43225.0
34	36015.0
35	26530.0
36	12947.0
37	2642.0
38	71.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.912016859832672	23.293635037250926	26.72104918156038	26.073298921356024
2	38.98530873276366	16.91175606810658	30.553042922558955	13.5498922765708
3	28.606267929431166	14.137026081522084	43.604611549549254	13.652094439497494
4	25.7720962250985	24.41832872444652	36.47865384479838	13.330921205656596
5	19.26334560542447	29.212432481961905	39.00950832536469	12.514713587248929
6	18.51174854978655	33.82656645372592	37.245522488000056	10.416162508487478
7	77.1269209895049	3.2448599360472903	18.154863295796556	1.4733557786512606
8	78.96092118216185	1.9768482732533417	17.31539587479178	1.7468346697930348
9	67.21331118561558	2.883510679538657	17.09783448888346	12.805343645962312
10	47.942682207660695	20.123958301517526	23.031433626156236	8.90192586466554
11	41.50300615327505	18.175538675882873	29.656013363813855	10.66544180702822
12	36.47207531477091	18.930659944035504	32.503812023203416	12.093452717990164
13	19.00678293435332	34.444948276307656	33.46662688222317	13.081641907115854
14	14.285747849642997	34.79149584025449	37.05732953971435	13.865426770388156
15	12.437885754428173	18.103174845580757	52.71775521759663	16.741184182394445
16	13.965044510804061	14.507303343067992	50.60839655379006	20.919255592337894
17	15.908765186419094	16.085680654657715	37.15929675514006	30.846257403783124
18	20.879079569670157	19.11885289232121	41.98793779529964	18.01412974270899
19	27.038228307884605	20.762075714181666	34.36553602097611	17.834159956957617
20	28.31610776571975	20.687127461368757	33.81012012865725	17.186644644254244
21	21.017933542749873	24.21227976608627	36.824261618741296	17.945525072422566
22	20.561900443345934	20.676554823824617	34.63008690708062	24.131457825748836
23	17.719270628977952	25.766692432575937	34.40618193864581	22.107854999800296
24	17.703999041414196	20.504808200607574	45.87890335904442	15.912289398933806
25	16.45689770620755	20.458758490415317	43.28437810571228	19.799965697664856
26	12.616680802674644	40.93137888338851	29.219950801993296	17.231989511943556
27	16.334020163194534	28.996045833558494	37.45086660385737	17.219067399389605
28	14.627831411071195	23.827435759479545	45.510035782504396	16.03469704694486
29	15.48468494714856	20.93946107742225	44.175533976932854	19.400319998496336
30	18.461234837075654	25.689629652254204	38.703841626588535	17.145293884081603
31	27.26824191134491	22.25516708291532	33.31320616408264	17.16338484165713
32	28.15258430503704	21.90180604144004	33.16918334598134	16.77642630754158
33	26.705777593996622	23.088290921393614	32.00736795363076	18.198563530979005
34	18.757738583313557	23.931752449915066	33.69523080067759	23.615278166093788
35	19.119792682325134	24.053220307922192	35.56470806598266	21.262278943770013
36	28.66899891219307	22.548616511640475	30.052134850467667	18.73024972569879
37	19.277677402984303	29.956041322566474	31.911979268232514	18.85430200621671
38	19.53306533655055	30.46117844967542	28.514168509046655	21.491587704727376
39	19.94657293827694	28.781538765162924	29.900358764833996	21.371529531726136
40	23.447290702892438	23.896980219769894	29.039981016241924	23.615748061095747
41	18.062293980410075	21.913788363990065	30.786815686034956	29.2371019695649
42	21.277550531333773	23.15548590667415	27.260018748810577	28.306944813181495
43	21.347799834127066	23.567348875893682	29.051728391290965	26.033122898688287
44	19.198030200151774	27.15452732087015	30.67098656805137	22.976455910926706
45	17.354867054956568	33.650825722992195	26.39118289018319	22.603124331868045
46	20.831150279470055	28.456136476304373	28.771436022620744	21.94127722160483
47	20.353971904977833	24.185495750974447	30.005615245273443	25.454917098774278
48	21.32289539902309	22.460981093774596	33.61699328285095	22.59913022435137
49	20.28043333717081	22.393081265991114	33.41564327451031	23.910842122327765
50	19.07938171215642	28.889379668113158	30.658534350499384	21.37270426923104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	143.0
1	267.0
2	391.0
3	4646.0
4	8901.0
5	7114.0
6	5327.0
7	5276.0
8	5225.0
9	5651.5
10	6078.0
11	6374.5
12	6671.0
13	6663.5
14	6656.0
15	6477.5
16	6299.0
17	5905.0
18	5511.0
19	5279.0
20	5047.0
21	4781.0
22	4515.0
23	4196.0
24	3877.0
25	3757.0
26	3637.0
27	3681.5
28	3726.0
29	4165.5
30	4605.0
31	5185.0
32	5765.0
33	6543.0
34	7321.0
35	7969.5
36	8618.0
37	9336.5
38	10055.0
39	13139.5
40	16224.0
41	21728.0
42	27232.0
43	32336.5
44	37441.0
45	38795.5
46	40150.0
47	36748.0
48	33346.0
49	31227.0
50	29108.0
51	26752.5
52	24397.0
53	22485.0
54	20573.0
55	19209.0
56	17845.0
57	16622.5
58	15400.0
59	14504.0
60	13608.0
61	12409.5
62	11211.0
63	10252.5
64	9294.0
65	7967.0
66	6640.0
67	5780.5
68	4921.0
69	4113.0
70	3305.0
71	2916.5
72	2528.0
73	2125.0
74	1722.0
75	1376.0
76	1030.0
77	800.5
78	571.0
79	454.5
80	338.0
81	264.0
82	190.0
83	138.0
84	86.0
85	62.5
86	39.0
87	38.5
88	38.0
89	26.0
90	14.0
91	9.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	425627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.26994652628913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.85016332276514	42.4772344325249
2	6.805534564962433	6.9783878645687265
3	2.651940073997603	4.078944773543411
4	1.5606476945763175	3.200572953892168
5	1.027067338091545	2.6328843751425812
6	0.7284482171185882	2.2408500683304404
7	0.5718132320632908	2.0521783681636636
8	0.4612827200779956	1.8919952313520025
9	0.37043663530079896	1.709303983492539
>10	2.8687142988899517	27.834153156125446
>50	0.09599386179991128	3.0995880808812104
>100	0.00746066283418956	0.5138354572583578
>500	0.0	0.0
>1k	0.0	0.0
>5k	4.97377522279304E-4	1.2900712547245814
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5059	1.1885994074624027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03406738764223134	0.0
2	0.0	0.0	0.0	0.1334501805571545	0.0
3	0.0	0.0	0.0	0.17597567823469845	0.0
4	0.0	0.0	0.0	0.2532734060574165	0.0
5	0.0	0.0	0.0	0.424080239270535	0.0
6	0.0	0.0	0.0	0.5892483324601118	0.0
7	0.0	0.0	0.0	0.7003785004240802	0.0
8	0.0	0.0	0.0	1.0621976519346752	0.0
9	0.0	0.0	0.0	1.2757649303263185	0.0
10	0.0	0.0	0.0	1.581901524104439	0.0
11	0.0	0.0	0.0	1.8093307050539558	0.0
12	0.0	0.0	0.0	2.0116205033985155	0.0
13	0.0	0.0	0.0	2.094791918745756	0.0
14	0.0	0.0	0.0	2.130034043892892	0.0
15	0.0	0.0	0.0	2.1666858540459133	0.0
16	0.0	0.0	0.0	2.232471154320567	0.0
17	0.0	0.0	0.0	2.3264501547129295	0.0
18	0.0	0.0	0.0	2.4561411752543894	0.0
19	0.0	0.0	0.0	2.5137033129947115	0.0
20	0.0	0.0	0.0	2.5830128257840785	0.0
21	0.0	0.0	0.0	2.649033073559713	0.0
22	0.0	0.0	0.0	2.7211619563608513	0.0
23	0.0	0.0	0.0	2.799164526686512	0.0
24	0.0	0.0	0.0	2.8574315069297764	0.0
25	0.0	0.0	0.0	2.903716164623015	0.0
26	0.0	0.0	0.0	2.9474163998054634	0.0
27	0.0	0.0	0.0	3.0113221200722697	0.0
28	0.0	0.0	0.0	3.075932682842019	0.0
29	0.0	0.0	0.0	3.1485314606451187	0.0
30	0.0	0.0	0.0	3.2425104610374813	0.0
31	0.0	0.0	0.0	3.32521198138276	0.0
32	0.0	0.0	0.0	3.394991389174089	0.0
33	0.0	0.0	0.0	3.4685299569811128	0.0
34	0.0	0.0	0.0	3.5484121073146206	0.0
35	0.0	0.0	0.0	3.6459153202216963	0.0
36	0.0	0.0	0.0	3.729086735568937	0.0
37	0.0	0.0	0.0	3.8103785709083304	0.0
38	0.0	0.0	0.0	3.90882157381933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTG	20	7.850887E-4	44.000004	1
CAACCCG	20	7.850887E-4	44.000004	23
TTCGTAG	20	7.850887E-4	44.000004	1
TCGATTA	30	2.523946E-6	44.000004	41
CGACAGG	40	8.290954E-9	44.000004	2
TTATCGG	20	7.850887E-4	44.000004	2
ACGGGTG	20	7.850887E-4	44.000004	5
ATGTACG	20	7.850887E-4	44.000004	1
CGAATAA	20	7.850887E-4	44.000004	13
CGTCGGG	40	8.290954E-9	44.000004	3
GCGTAAA	20	7.850887E-4	44.000004	1
TACGTAG	30	2.523946E-6	44.000004	1
TAGTACA	20	7.850887E-4	44.000004	41
GTCATTA	25	4.438102E-5	44.0	20
CGGGTAT	35	1.4432044E-7	44.0	6
TTGGCGA	25	4.438102E-5	44.0	5
TGATTCG	35	1.4432044E-7	44.0	15
ACTAACC	35	1.4432044E-7	44.0	14
CGTTTTT	6145	0.0	43.140766	1
CGGGAAT	920	0.0	41.369564	9
>>END_MODULE