FastQCFastQC Report
Thu 26 May 2016
SRR1545957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545957_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1026187
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179231.7465627609782621No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13540.1319447625042999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG207.8560854E-444.0000041
TCTAGCG207.8560854E-444.0000041
ACGCTTA207.8560854E-444.00000442
GCGTAAG950.044.0000041
AGCTTCG302.5274512E-644.01
ATGTCGA254.442506E-544.038
ACGGGTA302.5274512E-644.05
TACGATG302.5274512E-644.01
ATATACG302.5274512E-644.01
CGGCATA351.4458783E-743.99999634
TATTACG351.4458783E-743.9999961
CGCACGG351.4458783E-743.9999962
CGTTTTT130150.043.0703051
TTAACGG1500.042.5333332
CGTAAGG1450.042.4827582
TACGGGT950.041.684214
GCGTTAG850.041.4117621
ACGGGAT2400.041.255
ATGGGCG2550.040.5490155
TATACGG603.6379788E-1240.3333322
Produced by FastQC (version 0.11.5)