##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545957_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1026187
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.07630578052538	31.0	28.0	33.0	23.0	33.0
2	28.863326079944493	31.0	28.0	33.0	16.0	33.0
3	28.283266110367798	31.0	27.0	33.0	16.0	34.0
4	32.22685826267532	35.0	32.0	35.0	25.0	37.0
5	33.71127874354284	35.0	33.0	35.0	28.0	37.0
6	33.516276273232855	35.0	33.0	37.0	28.0	37.0
7	34.45062059838996	35.0	35.0	37.0	32.0	37.0
8	34.91773624105548	35.0	35.0	37.0	32.0	37.0
9	36.74491393868759	39.0	37.0	39.0	32.0	39.0
10	36.51273500833669	39.0	35.0	39.0	32.0	39.0
11	36.44849330580099	39.0	35.0	39.0	32.0	39.0
12	35.11432614133681	37.0	34.0	39.0	30.0	39.0
13	35.0134488158591	37.0	34.0	39.0	27.0	39.0
14	35.712258097208405	38.0	33.0	40.0	27.0	41.0
15	36.405265317140056	38.0	35.0	40.0	31.0	41.0
16	36.07566262289427	37.0	34.0	40.0	31.0	41.0
17	35.685078840406284	37.0	34.0	40.0	30.0	41.0
18	35.343712208398664	37.0	34.0	39.0	29.0	40.0
19	34.77502346063632	36.0	33.0	39.0	27.0	40.0
20	35.179235363535106	35.0	34.0	39.0	30.0	40.0
21	35.34622831901008	35.0	34.0	39.0	30.0	40.0
22	35.67784331705625	35.0	34.0	39.0	31.0	41.0
23	36.05100727255364	35.0	35.0	40.0	32.0	41.0
24	35.73528898728984	35.0	35.0	40.0	31.0	41.0
25	35.35490315118005	35.0	34.0	39.0	30.0	41.0
26	35.465292388229436	35.0	34.0	40.0	31.0	41.0
27	35.72614348067165	35.0	34.0	40.0	31.0	41.0
28	35.68128810830775	36.0	35.0	40.0	31.0	41.0
29	35.83788432322764	36.0	35.0	40.0	31.0	41.0
30	35.49476362495335	36.0	35.0	40.0	31.0	41.0
31	34.77828797285485	35.0	34.0	39.0	29.0	41.0
32	34.63445064106249	35.0	34.0	40.0	27.0	41.0
33	34.3076661466185	35.0	34.0	40.0	24.0	41.0
34	33.939250838297504	35.0	34.0	40.0	22.0	41.0
35	33.63515421653168	35.0	33.0	40.0	19.0	41.0
36	33.19086287392064	35.0	33.0	40.0	18.0	41.0
37	33.0692125314392	35.0	33.0	40.0	17.0	41.0
38	32.93473411766082	35.0	33.0	40.0	16.0	41.0
39	32.77807748490285	35.0	33.0	39.0	15.0	40.0
40	32.57458046145585	35.0	32.0	39.0	15.0	40.0
41	32.303415459365596	35.0	32.0	39.0	13.0	40.0
42	32.44806843197195	35.0	32.0	39.0	12.0	40.0
43	32.22300126585115	35.0	32.0	39.0	12.0	40.0
44	32.05087571758364	35.0	32.0	39.0	10.0	40.0
45	32.02555674550545	35.0	31.0	39.0	10.0	40.0
46	31.911098074717376	35.0	31.0	38.0	10.0	40.0
47	31.894647856579745	35.0	31.0	38.0	10.0	40.0
48	31.91870195198341	35.0	31.0	38.0	10.0	40.0
49	31.9176280736357	35.0	31.0	38.0	10.0	40.0
50	32.009828617980936	35.0	32.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	3.0
13	14.0
14	38.0
15	119.0
16	246.0
17	607.0
18	1130.0
19	1938.0
20	3189.0
21	4592.0
22	6614.0
23	9566.0
24	13844.0
25	20929.0
26	29675.0
27	34253.0
28	32119.0
29	30476.0
30	32728.0
31	39474.0
32	53810.0
33	85495.0
34	141312.0
35	97036.0
36	87144.0
37	123956.0
38	142481.0
39	33391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.258774472878727	22.366781103249213	27.148950434959712	23.22549398891235
2	37.79184495613372	20.022276641586767	29.56683333544471	12.619045066834797
3	27.402023217990486	19.089015939589956	40.58889851459821	12.920062327821341
4	24.06705600441245	25.560351086108085	36.85760977287765	13.514983136601808
5	20.11933497500943	29.48692587218509	37.523765161710294	12.86997399109519
6	18.26947720054922	36.78900629222549	34.740646685253274	10.200869821972018
7	77.27987199214178	4.4317458708792845	16.194514255199103	2.0938678817798313
8	79.00986857171256	2.8327195725535406	15.940174646531286	2.217237209202611
9	75.55016775694878	4.467216988716482	17.136935081032988	2.845680173301747
10	47.87480254573484	21.194090355851323	21.465385938430327	9.465721159983511
11	42.50346184467354	17.83495600704355	28.910422759204707	10.75115938907821
12	38.67384794389327	19.274654619479687	30.071906972121067	11.979590464505982
13	19.772322198585638	36.65949773286935	30.98489846392519	12.58328160461982
14	13.64955899850612	36.974644972115215	35.69290977180572	13.682886257572937
15	12.830994740724645	20.038940271120175	53.714673836250114	13.415391151905062
16	15.006816496408549	16.526325123978378	51.21854009064625	17.24831828896683
17	15.853640710708671	16.81642819486117	36.180442745815334	31.149488348614824
18	20.380398504366163	20.075483318342563	41.2507661858901	18.293351991401178
19	26.39967179471188	21.711832248898105	33.2087621456908	18.67973381069922
20	27.986224732919045	21.40165486407448	33.06697512246794	17.545145280538538
21	19.86372854070457	25.382508256292468	36.043040888259156	18.710722314743805
22	20.443642338092374	21.66846783286087	33.9294884850422	23.958401344004553
23	17.425576430026886	27.251660759686104	33.3085490266394	22.01421378364762
24	17.7512480668728	20.970641803102165	45.727825435325144	15.550284694699895
25	15.803552373982518	22.538679597383325	42.42706251394726	19.230705514686893
26	14.785414354303844	31.063636549673696	35.36421724305609	18.78673185296637
27	15.950406699753552	30.0705426983581	36.363157981927266	17.61589261996108
28	13.519075957890717	25.692490744864237	44.50952896499371	16.278904332251333
29	14.448048942346764	22.19098468407805	43.94929968904303	19.411666684532154
30	17.85425073597697	26.923942712195732	37.854991341734014	17.366815210093286
31	26.377843414504376	23.458882250506	32.951304196993334	17.21197013799629
32	27.54117914181333	23.565295604017592	33.00743431752692	15.886090936642153
33	26.02235265112499	24.9121261524459	31.367869598815812	17.6976515976133
34	17.786621736584074	25.127876303246875	34.51339765559299	22.572104304576065
35	18.854458300485195	25.213143413432444	35.80916538603588	20.123232900046485
36	28.95856213341233	23.708057108499716	29.57326491175585	17.7601158463321
37	18.93348873061148	31.235145251304097	31.77335125079542	18.058014767289002
38	20.130444061365033	30.51383422319714	28.58377664109953	20.7719450743383
39	20.170008000491137	29.53837848267421	30.010806997165236	20.28080651966942
40	23.917862923619186	24.745977097741445	28.46976233376568	22.866397644873693
41	17.391274689700804	23.525829113017412	30.482553374774774	28.60034282250701
42	21.292707859288804	23.612168152588172	28.14730648507533	26.947817503047688
43	21.39337177337074	24.574078603607337	29.17002456667255	24.86252505634938
44	18.933001489981844	28.28597516826855	30.69957035121279	22.081452990536814
45	16.817987364875993	34.702349571764216	25.99009732144336	22.489565741916433
46	20.55775409355215	30.088083361024843	28.883527076449028	20.470635468973978
47	20.398036615158833	25.40716263215184	30.789514971442827	23.405285781246498
48	21.81795325803192	22.622582433805924	33.750476277715464	21.808988030446695
49	20.76210281361974	23.14461204439347	33.78380353678228	22.30948160520451
50	18.55285635074309	30.7162339807462	29.894161590431374	20.83674807807934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	464.0
1	813.5
2	1163.0
3	14652.5
4	28142.0
5	19949.5
6	11757.0
7	12254.0
8	12751.0
9	13575.5
10	14400.0
11	14442.0
12	14484.0
13	13759.5
14	13035.0
15	12170.5
16	11306.0
17	10523.5
18	9741.0
19	8926.5
20	8112.0
21	7490.0
22	6868.0
23	7164.0
24	7460.0
25	7129.0
26	6798.0
27	7750.5
28	8703.0
29	9909.0
30	11115.0
31	13387.0
32	15659.0
33	17768.5
34	19878.0
35	21961.5
36	24045.0
37	26821.5
38	29598.0
39	37738.0
40	45878.0
41	60144.0
42	74410.0
43	89246.0
44	104082.0
45	103826.5
46	103571.0
47	95443.5
48	87316.0
49	79751.5
50	72187.0
51	67577.0
52	62967.0
53	57067.5
54	51168.0
55	45519.0
56	39870.0
57	36289.0
58	32708.0
59	30135.0
60	27562.0
61	24390.5
62	21219.0
63	18789.0
64	16359.0
65	13454.5
66	10550.0
67	8767.0
68	6984.0
69	5913.5
70	4843.0
71	4172.5
72	3502.0
73	2981.5
74	2461.0
75	1914.0
76	1367.0
77	1017.0
78	667.0
79	532.5
80	398.0
81	349.0
82	300.0
83	227.0
84	154.0
85	126.0
86	98.0
87	74.5
88	51.0
89	33.5
90	16.0
91	12.5
92	9.0
93	8.0
94	7.0
95	4.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1026187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.139654199470865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53999315148421	21.044086007606204
2	7.207693460931748	3.9122861621094995
3	3.0701797127157735	2.4997084720001066
4	1.9315946080552564	2.0969123886472847
5	1.2696402795213446	1.722879907196441
6	0.9676281352864058	1.5756655791211118
7	0.7632735888024427	1.4500486881781187
8	0.5846123158818639	1.269294087502849
9	0.4789526131365541	1.169874746861312
>10	4.77368607708972	29.19588681692223
>50	1.0912245483576581	20.736597823375114
>100	0.32079979940199765	11.438871936619869
>500	0.0	0.0
>1k	3.608546674938079E-4	0.1326035958783162
>5k	0.0	0.0
>10k+	3.608546674938079E-4	1.7552837879815812
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17923	1.7465627609782621	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1354	0.1319447625042999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03907669849647286	9.744812592636625E-5
2	0.0	0.0	0.0	0.1151836848449649	9.744812592636625E-5
3	0.0	0.0	0.0	0.15406548708958503	9.744812592636625E-5
4	0.0	0.0	0.0	0.23338826159364717	9.744812592636625E-5
5	0.0	0.0	0.0	0.39827049066105885	9.744812592636625E-5
6	0.0	0.0	0.0	0.5976493563064041	9.744812592636625E-5
7	0.0	0.0	0.0	0.7396312757811199	9.744812592636625E-5
8	0.0	0.0	0.0	1.166161722960825	9.744812592636625E-5
9	0.0	0.0	0.0	1.3720696130432368	9.744812592636625E-5
10	0.0	0.0	0.0	1.6941356692298772	9.744812592636625E-5
11	0.0	0.0	0.0	1.9237234539123962	9.744812592636625E-5
12	0.0	0.0	0.0	2.145320492268953	9.744812592636625E-5
13	9.744812592636625E-5	0.0	0.0	2.2385783487804853	9.744812592636625E-5
14	9.744812592636625E-5	0.0	0.0	2.272587744728787	9.744812592636625E-5
15	9.744812592636625E-5	0.0	0.0	2.3192653970475168	9.744812592636625E-5
16	9.744812592636625E-5	0.0	0.0	2.410379394788669	9.744812592636625E-5
17	9.744812592636625E-5	0.0	0.0	2.5328716890781116	9.744812592636625E-5
18	9.744812592636625E-5	0.0	0.0	2.716561406449312	9.744812592636625E-5
19	9.744812592636625E-5	0.0	0.0	2.7952020440718894	9.744812592636625E-5
20	9.744812592636625E-5	0.0	0.0	2.8790074323685646	9.744812592636625E-5
21	1.948962518527325E-4	0.0	0.0	2.9815228608431017	9.744812592636625E-5
22	1.948962518527325E-4	0.0	0.0	3.0881311106065463	9.744812592636625E-5
23	1.948962518527325E-4	0.0	0.0	3.204289276710775	9.744812592636625E-5
24	1.948962518527325E-4	0.0	0.0	3.2962803075852647	9.744812592636625E-5
25	3.89792503705465E-4	0.0	0.0	3.365565925118911	9.744812592636625E-5
26	3.89792503705465E-4	0.0	0.0	3.4433295296081514	9.744812592636625E-5
27	3.89792503705465E-4	0.0	0.0	3.5327869092085558	9.744812592636625E-5
28	3.89792503705465E-4	0.0	0.0	3.6187361562756104	9.744812592636625E-5
29	3.89792503705465E-4	0.0	0.0	3.7057573327278557	9.744812592636625E-5
30	3.89792503705465E-4	0.0	0.0	3.8341939626988064	9.744812592636625E-5
31	3.89792503705465E-4	0.0	0.0	3.9422639343511463	9.744812592636625E-5
32	3.89792503705465E-4	0.0	0.0	4.0363988239960165	9.744812592636625E-5
33	3.89792503705465E-4	0.0	0.0	4.133749501796456	9.744812592636625E-5
34	3.89792503705465E-4	0.0	0.0	4.2349006565080245	9.744812592636625E-5
35	3.89792503705465E-4	0.0	0.0	4.367137763390104	9.744812592636625E-5
36	4.872406296318312E-4	0.0	0.0	4.469555743738714	9.744812592636625E-5
37	5.846887555581975E-4	0.0	0.0	4.5839598435762685	9.744812592636625E-5
38	5.846887555581975E-4	0.0	0.0	4.718048464850948	9.744812592636625E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	20	7.8560854E-4	44.000004	1
TCTAGCG	20	7.8560854E-4	44.000004	1
ACGCTTA	20	7.8560854E-4	44.000004	42
GCGTAAG	95	0.0	44.000004	1
AGCTTCG	30	2.5274512E-6	44.0	1
ATGTCGA	25	4.442506E-5	44.0	38
ACGGGTA	30	2.5274512E-6	44.0	5
TACGATG	30	2.5274512E-6	44.0	1
ATATACG	30	2.5274512E-6	44.0	1
CGGCATA	35	1.4458783E-7	43.999996	34
TATTACG	35	1.4458783E-7	43.999996	1
CGCACGG	35	1.4458783E-7	43.999996	2
CGTTTTT	13015	0.0	43.070305	1
TTAACGG	150	0.0	42.533333	2
CGTAAGG	145	0.0	42.482758	2
TACGGGT	95	0.0	41.68421	4
GCGTTAG	85	0.0	41.411762	1
ACGGGAT	240	0.0	41.25	5
ATGGGCG	255	0.0	40.549015	5
TATACGG	60	3.6379788E-12	40.333332	2
>>END_MODULE