FastQCFastQC Report
Thu 26 May 2016
SRR1545956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545956_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1024092
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181291.7702511102518133No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14170.13836647488702186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG551.8189894E-1244.0000041
GTTTCGT207.85608E-444.043
ACGTTAG254.4424993E-544.01
GACGTAA207.85608E-444.012
TCGATCT207.85608E-444.022
ACGCTTA408.310963E-944.042
GCGATAT700.044.09
TATACGA207.85608E-444.044
TATCGAT302.5274458E-644.013
GTGCGAT207.85608E-444.011
AATACGG207.85608E-444.02
GCGTAAG1000.044.01
TGGTCGA302.5274458E-644.020
ATCGATT302.5274458E-644.014
ATCGACG254.4424993E-544.01
TGTTACG650.043.9999961
CGTTTTT128250.043.4682241
TGGGCGA5350.041.532716
GTTAGCG800.041.251
CGTAAGG1550.041.1612932
Produced by FastQC (version 0.11.5)