FastQCFastQC Report
Thu 26 May 2016
SRR1545955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545955_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1009962
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180061.782839354351946No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14040.1390151312623643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.8560266E-444.00000420
CTATGCG207.8560266E-444.0000041
ACGTTAG207.8560266E-444.0000041
ACGTTAC207.8560266E-444.00000418
ATCGTAA207.8560266E-444.00000423
CTAACGG207.8560266E-444.0000042
TCCACGC207.8560266E-444.00000437
GCGATAT302.5274094E-644.0000049
CAATCGT207.8560266E-444.00000424
CCACGAA207.8560266E-444.00000428
CGCTTAT207.8560266E-444.00000438
CGAACCG254.4424545E-544.026
GAGTACG454.802132E-1044.01
TACGGGA2050.044.04
TGTTACG254.4424545E-544.01
CACTTCG254.4424545E-544.028
ACGAACT254.4424545E-544.01
ATATACG351.4458601E-743.9999961
TATTACG351.4458601E-743.9999961
CGTTTTT129000.043.2837221
Produced by FastQC (version 0.11.5)