##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545955_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1009962
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.94006210134639	31.0	28.0	33.0	23.0	33.0
2	28.720780583823945	31.0	28.0	33.0	16.0	33.0
3	28.162290264386186	31.0	26.0	33.0	16.0	34.0
4	32.09522536491472	35.0	32.0	35.0	25.0	37.0
5	33.675717502242655	35.0	33.0	35.0	28.0	37.0
6	33.523932583602154	35.0	33.0	37.0	28.0	37.0
7	34.435694610292266	35.0	35.0	37.0	32.0	37.0
8	34.93274796477491	35.0	35.0	37.0	32.0	37.0
9	36.85614409255002	39.0	37.0	39.0	32.0	39.0
10	36.58501805018407	39.0	35.0	39.0	32.0	39.0
11	36.50445165263643	39.0	35.0	39.0	32.0	39.0
12	35.22515797624069	37.0	34.0	39.0	30.0	39.0
13	35.111980450749634	37.0	34.0	39.0	27.0	39.0
14	35.79431701390745	38.0	34.0	40.0	27.0	41.0
15	36.42763688138761	38.0	35.0	40.0	31.0	41.0
16	36.07498995011694	37.0	34.0	40.0	31.0	41.0
17	35.70419976197124	37.0	34.0	40.0	30.0	41.0
18	35.35651539364847	37.0	34.0	39.0	29.0	40.0
19	34.74521615664748	36.0	33.0	39.0	27.0	40.0
20	35.24256853228141	35.0	34.0	39.0	30.0	40.0
21	35.335048249340076	35.0	34.0	39.0	30.0	40.0
22	35.680122618474755	35.0	34.0	39.0	31.0	41.0
23	36.06086565633162	35.0	35.0	40.0	32.0	41.0
24	35.65799505327923	35.0	34.0	40.0	31.0	41.0
25	35.300470710779216	35.0	34.0	39.0	30.0	41.0
26	35.44963473873275	35.0	34.0	39.0	31.0	41.0
27	35.750014356975804	35.0	35.0	40.0	31.0	41.0
28	35.712617900475465	36.0	35.0	40.0	31.0	41.0
29	35.86273939019488	36.0	35.0	40.0	31.0	41.0
30	35.53686673359988	36.0	35.0	40.0	31.0	41.0
31	34.79503981337912	35.0	34.0	39.0	29.0	41.0
32	34.70587408239122	35.0	34.0	40.0	28.0	41.0
33	34.4056677379941	35.0	34.0	40.0	25.0	41.0
34	33.98415286911785	35.0	34.0	40.0	22.0	41.0
35	33.67687794194237	35.0	33.0	40.0	20.0	41.0
36	33.26375645816377	35.0	33.0	40.0	18.0	41.0
37	33.17231242363574	35.0	33.0	40.0	18.0	41.0
38	33.04514031220977	35.0	33.0	40.0	17.0	41.0
39	32.82842126733481	35.0	33.0	40.0	15.0	41.0
40	32.64302023244439	35.0	32.0	39.0	15.0	40.0
41	32.34538230151234	35.0	32.0	39.0	14.0	40.0
42	32.53223883670871	35.0	32.0	39.0	13.0	40.0
43	32.318303064867784	35.0	32.0	39.0	12.0	40.0
44	32.1467084900224	35.0	32.0	39.0	10.0	40.0
45	32.11934112372545	35.0	32.0	39.0	10.0	40.0
46	31.990304585717087	35.0	31.0	39.0	10.0	40.0
47	31.996820672460945	35.0	32.0	38.0	10.0	40.0
48	32.00185947590107	35.0	32.0	38.0	10.0	40.0
49	32.0641529087233	35.0	32.0	38.0	10.0	40.0
50	32.11260423659504	35.0	32.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	4.0
12	8.0
13	15.0
14	32.0
15	107.0
16	242.0
17	529.0
18	1014.0
19	1915.0
20	2970.0
21	4397.0
22	6523.0
23	9059.0
24	13533.0
25	20469.0
26	29138.0
27	33399.0
28	31108.0
29	29919.0
30	31844.0
31	38906.0
32	52333.0
33	84548.0
34	139253.0
35	94149.0
36	85855.0
37	122656.0
38	142523.0
39	33512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.386574940443303	22.23539103451417	27.086959707394932	23.291074317647595
2	37.8753854105402	19.97154348381424	29.51903140910252	12.63403969654304
3	27.38736704945335	19.103292995182	40.55023852382565	12.959101431539008
4	23.947930714224892	25.644034131977243	36.87010006317069	13.537935090627172
5	20.18323461674796	29.501704024507852	37.504183325709285	12.810878033034905
6	18.209397977349642	36.970202839314744	34.73605937649139	10.084339806844218
7	77.28855145045061	4.42303769844806	16.15595438244211	2.132456468659217
8	79.08446060346826	2.820898212011937	15.922183210853477	2.172457973666336
9	75.70997720706323	4.444127600840428	17.102326622189747	2.7435685699065906
10	47.776252967933445	21.220699392650417	21.488333224418344	9.514714414997792
11	42.42832898663514	17.831561979559627	28.994358203575977	10.745750830229255
12	38.6275919292013	19.30023109780368	30.041229273972686	12.030947699022338
13	19.730940372013997	36.57900000198027	31.041167885524406	12.648891740481325
14	13.73893275192532	36.864258259221636	35.689857638208174	13.706951350644875
15	12.873652672080732	20.090458848946792	53.66360318507033	13.372285293902147
16	15.075022624613599	16.497551393022707	51.14439949225812	17.28302649010557
17	15.827130129648442	16.923012945041496	36.1322505203166	31.117606404993452
18	20.223236121755075	20.198482715191265	41.2372940764108	18.340987086642862
19	26.35604111837871	21.693192417140448	33.20590279634283	18.74486366813801
20	27.969567171834186	21.313970228582857	33.06797681496928	17.64848578461368
21	19.97599909699573	25.347785362221547	36.03204873054629	18.644166810236428
22	20.431560791396112	21.724678750289613	33.92088019153196	23.922880266782315
23	17.41520968115632	27.25280753137247	33.32689744762674	22.00508533984447
24	17.689774466762113	20.967125495810734	45.69399640778564	15.64910362964151
25	15.777920357399585	22.586196312336504	42.46575613735962	19.17012719290429
26	14.814418760309794	30.985423213942703	35.414203702713564	18.785954323033934
27	15.873072452230877	30.115588507290376	36.2869098045273	17.72442923595145
28	13.535756790849558	25.72987894594054	44.49682265273347	16.237541610476434
29	14.418562282541322	22.095385766989253	43.948188149653156	19.537863800816268
30	17.778688703139327	26.992995776078704	37.885781841297	17.34253367948497
31	26.31524750436155	23.420683154415713	32.94143740061507	17.322631940607668
32	27.462914446286096	23.578015806535298	33.0486691578495	15.910400589329104
33	26.00711709945523	24.91875931965757	31.387418536539	17.6867050443482
34	17.81175925430858	25.214513021281988	34.47307918515746	22.500648539251973
35	18.788132622811553	25.162926922003003	35.89105332675883	20.157887128426612
36	28.933959891560278	23.713565460878726	29.642204360164047	17.710270287396952
37	18.96972361336367	31.173648117453922	31.8584263566352	17.998201912547206
38	20.224028230765118	30.419659353520235	28.69404987514382	20.662262540570833
39	20.183927712131744	29.600717650763098	30.007960695550924	20.207393941554237
40	23.933771765670393	24.814002903079523	28.45077339543468	22.801451935815408
41	17.44540883716417	23.55732195864795	30.479760624657164	28.517508579530716
42	21.151785908776766	23.770498295975493	28.25858794687325	26.819127848374492
43	21.383378780587783	24.681720698402515	29.09980771553781	24.835092805471888
44	19.022299848905206	28.258686960499503	30.752939219495385	21.966073971099902
45	16.74033280460057	34.720217196290555	26.173658018816546	22.365791980292325
46	20.615825149857123	30.10836051257374	29.0221810325537	20.253633305015438
47	20.504929888451247	25.448482220123132	30.79749535131025	23.24909254011537
48	21.83557401169549	22.673427317067375	33.78493448268351	21.70606418855363
49	20.865636528899106	23.171564870757514	33.874541814444505	22.088256785898874
50	18.639216128923664	30.80145589636046	29.87270808208626	20.686619892629622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	462.0
1	807.5
2	1153.0
3	14714.0
4	28275.0
5	20134.0
6	11993.0
7	12402.0
8	12811.0
9	13652.5
10	14494.0
11	14299.5
12	14105.0
13	13382.5
14	12660.0
15	11677.0
16	10694.0
17	9959.5
18	9225.0
19	8521.5
20	7818.0
21	7291.5
22	6765.0
23	6938.5
24	7112.0
25	6810.0
26	6508.0
27	7481.5
28	8455.0
29	9722.0
30	10989.0
31	13295.5
32	15602.0
33	17654.5
34	19707.0
35	21742.5
36	23778.0
37	26476.0
38	29174.0
39	37253.5
40	45333.0
41	59349.0
42	73365.0
43	88296.0
44	103227.0
45	102138.5
46	101050.0
47	93246.0
48	85442.0
49	78068.5
50	70695.0
51	66416.5
52	62138.0
53	56123.5
54	50109.0
55	44836.0
56	39563.0
57	35902.0
58	32241.0
59	29653.5
60	27066.0
61	23935.0
62	20804.0
63	18516.0
64	16228.0
65	13335.5
66	10443.0
67	8635.5
68	6828.0
69	5748.0
70	4668.0
71	4113.5
72	3559.0
73	2970.5
74	2382.0
75	1839.5
76	1297.0
77	999.5
78	702.0
79	559.5
80	417.0
81	356.5
82	296.0
83	225.0
84	154.0
85	126.5
86	99.0
87	69.0
88	39.0
89	26.0
90	13.0
91	11.0
92	9.0
93	8.0
94	7.0
95	5.0
96	3.0
97	4.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1009962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.64754760064066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.975134053728	20.511985487624123
2	7.324759240811061	3.903737410654905
3	3.2190661162624763	2.573406526881413
4	1.8940391929899234	2.01885998210712
5	1.382146035791596	1.8415401139896668
6	1.0251775693180338	1.6391080846506836
7	0.772577494437873	1.4411106890751841
8	0.5923090893125866	1.2626867721399415
9	0.4767480193301293	1.1433748984749694
>10	4.894999109728474	29.700045606897802
>50	1.1236144181055725	20.947137332222496
>100	0.31868158135483665	11.082367148885753
>500	0.0	0.0
>1k	3.7403941473572303E-4	0.13993995284595076
>5k	0.0	0.0
>10k+	3.7403941473572303E-4	1.7946999935499925
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18006	1.782839354351946	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1404	0.1390151312623643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03604095995690927	0.0
2	0.0	0.0	0.0	0.11148934316340615	0.0
3	0.0	0.0	0.0	0.15327309344311965	0.0
4	0.0	0.0	0.0	0.2302066810434452	0.0
5	9.901362625524525E-5	0.0	0.0	0.3846679380016278	0.0
6	9.901362625524525E-5	0.0	0.0	0.5752691685429748	0.0
7	9.901362625524525E-5	0.0	0.0	0.7176507630980176	0.0
8	9.901362625524525E-5	0.0	0.0	1.1242007125020546	0.0
9	1.980272525104905E-4	0.0	0.0	1.3253964010527128	0.0
10	2.9704087876573573E-4	0.0	0.0	1.6416459233119662	0.0
11	2.9704087876573573E-4	0.0	0.0	1.864822636891289	0.0
12	2.9704087876573573E-4	0.0	0.0	2.0842368326729126	0.0
13	2.9704087876573573E-4	0.0	0.0	2.1722599464138255	0.0
14	2.9704087876573573E-4	0.0	0.0	2.2071127428556716	0.0
15	2.9704087876573573E-4	0.0	0.0	2.252262956428064	0.0
16	2.9704087876573573E-4	0.0	0.0	2.339989029290211	0.0
17	2.9704087876573573E-4	0.0	0.0	2.450983304322341	0.0
18	2.9704087876573573E-4	0.0	0.0	2.631881199490674	0.0
19	2.9704087876573573E-4	0.0	0.0	2.7064384600608737	0.0
20	2.9704087876573573E-4	0.0	0.0	2.7910951105091084	0.0
21	2.9704087876573573E-4	0.0	0.0	2.890207750390609	0.0
22	2.9704087876573573E-4	0.0	0.0	2.9908055946659378	0.0
23	2.9704087876573573E-4	0.0	0.0	3.1004136789304946	0.0
24	2.9704087876573573E-4	0.0	0.0	3.182594988722348	0.0
25	2.9704087876573573E-4	0.0	0.0	3.25002326820217	0.0
26	2.9704087876573573E-4	0.0	0.0	3.323194338004796	0.0
27	2.9704087876573573E-4	0.0	0.0	3.4073559203217547	0.0
28	2.9704087876573573E-4	0.0	0.0	3.4817151536394437	0.0
29	3.96054505020981E-4	0.0	0.0	3.564787586067595	0.0
30	3.96054505020981E-4	0.0	0.0	3.6902378505329905	0.0
31	3.96054505020981E-4	0.0	0.0	3.792122871949638	0.0
32	4.950681312762262E-4	0.0	0.0	3.8777696586604247	0.0
33	5.940817575314715E-4	0.0	0.0	3.9692582493202715	0.0
34	5.940817575314715E-4	0.0	0.0	4.069162998211814	0.0
35	5.940817575314715E-4	0.0	0.0	4.197187616959846	0.0
36	5.940817575314715E-4	0.0	0.0	4.292240698164881	0.0
37	5.940817575314715E-4	0.0	0.0	4.402739905065735	0.0
38	6.930953837867167E-4	0.0	0.0	4.534923096116487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.8560266E-4	44.000004	20
CTATGCG	20	7.8560266E-4	44.000004	1
ACGTTAG	20	7.8560266E-4	44.000004	1
ACGTTAC	20	7.8560266E-4	44.000004	18
ATCGTAA	20	7.8560266E-4	44.000004	23
CTAACGG	20	7.8560266E-4	44.000004	2
TCCACGC	20	7.8560266E-4	44.000004	37
GCGATAT	30	2.5274094E-6	44.000004	9
CAATCGT	20	7.8560266E-4	44.000004	24
CCACGAA	20	7.8560266E-4	44.000004	28
CGCTTAT	20	7.8560266E-4	44.000004	38
CGAACCG	25	4.4424545E-5	44.0	26
GAGTACG	45	4.802132E-10	44.0	1
TACGGGA	205	0.0	44.0	4
TGTTACG	25	4.4424545E-5	44.0	1
CACTTCG	25	4.4424545E-5	44.0	28
ACGAACT	25	4.4424545E-5	44.0	1
ATATACG	35	1.4458601E-7	43.999996	1
TATTACG	35	1.4458601E-7	43.999996	1
CGTTTTT	12900	0.0	43.283722	1
>>END_MODULE