FastQCFastQC Report
Thu 26 May 2016
SRR1545954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545954_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences904095
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95931.0610610610610611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA207.8555883E-444.0000046
TCGTTGC207.8555883E-444.00000428
ACCGGTA207.8555883E-444.00000440
CGAACGG207.8555883E-444.0000042
AGTCACG207.8555883E-444.0000041
TAGCACG207.8555883E-444.0000041
GGAACGT207.8555883E-444.0000048
TCGTCGG207.8555883E-444.0000042
TCGGTCA207.8555883E-444.00000416
TACGTTG408.309144E-944.0000041
TAGTACG207.8555883E-444.0000041
TCGTTCA351.4456236E-744.016
CGAAAGG750.044.02
TAACGGC551.8189894E-1244.03
ATATCGA351.4456236E-744.018
CCGAACT254.4420834E-544.012
CCGTTTG302.5271147E-644.01
ATACCGG502.7284841E-1144.02
ATCGACG302.5271147E-644.01
ACGAACT351.4456236E-744.01
Produced by FastQC (version 0.11.5)