##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545954_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	904095
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.934491397474822	28.0	25.0	30.0	16.0	33.0
2	26.109284975583318	30.0	25.0	31.0	10.0	33.0
3	26.354018106504295	30.0	25.0	31.0	16.0	33.0
4	28.093914909384523	32.0	19.0	35.0	10.0	37.0
5	31.2666755152943	33.0	30.0	35.0	19.0	37.0
6	29.774769244382504	33.0	28.0	35.0	17.0	37.0
7	29.786617556783302	32.0	28.0	35.0	17.0	35.0
8	32.55176170645784	33.0	32.0	35.0	28.0	35.0
9	27.143111066867974	31.0	23.0	34.0	10.0	35.0
10	31.903000237806868	34.0	31.0	37.0	18.0	37.0
11	33.90292723662889	35.0	32.0	37.0	27.0	39.0
12	33.15616279262688	35.0	31.0	37.0	25.0	39.0
13	32.986475978741176	35.0	31.0	38.0	22.0	39.0
14	32.69209209209209	35.0	30.0	38.0	21.0	40.0
15	31.830745662789862	34.0	27.0	38.0	18.0	40.0
16	30.46022818398509	33.0	27.0	37.0	17.0	40.0
17	30.581800585115502	32.0	26.0	37.0	17.0	40.0
18	31.531985023697732	34.0	29.0	38.0	18.0	39.0
19	31.186823287375773	34.0	27.0	37.0	18.0	39.0
20	31.962200874908056	34.0	30.0	37.0	22.0	39.0
21	32.686792870218284	34.0	31.0	37.0	23.0	39.0
22	33.03181855889038	34.0	31.0	37.0	25.0	39.0
23	34.38594174284782	35.0	33.0	38.0	29.0	40.0
24	34.034037352269394	35.0	33.0	38.0	27.0	40.0
25	32.78404924261278	35.0	31.0	37.0	23.0	39.0
26	26.515299830216957	33.0	21.0	36.0	0.0	39.0
27	29.671232558525375	34.0	24.0	37.0	14.0	39.0
28	31.251391723214926	34.0	28.0	36.0	20.0	39.0
29	32.59974338979864	34.0	30.0	37.0	23.0	39.0
30	32.35061359702244	34.0	31.0	37.0	23.0	39.0
31	31.208232541934198	34.0	29.0	36.0	20.0	38.0
32	31.4146367361837	34.0	30.0	37.0	20.0	39.0
33	31.215909832484417	34.0	30.0	37.0	18.0	39.0
34	31.190100597835404	34.0	30.0	37.0	16.0	39.0
35	30.366906132651987	34.0	29.0	37.0	12.0	39.0
36	30.010168179228952	34.0	27.0	37.0	10.0	39.0
37	30.025932009357422	34.0	27.0	37.0	10.0	39.0
38	29.82596850994641	33.0	27.0	36.0	10.0	38.0
39	29.740549389168173	33.0	27.0	36.0	10.0	39.0
40	29.658634324932667	33.0	27.0	36.0	10.0	38.0
41	29.096642498852443	33.0	25.0	36.0	10.0	38.0
42	29.27534274606098	33.0	26.0	36.0	10.0	38.0
43	28.788398343094475	33.0	25.0	35.0	9.0	38.0
44	28.550819327614906	32.0	24.0	35.0	9.0	38.0
45	28.097974217311233	32.0	23.0	35.0	9.0	38.0
46	27.7547271027934	31.0	23.0	35.0	8.0	38.0
47	27.90920533793462	32.0	23.0	35.0	8.0	38.0
48	27.985003788318707	32.0	23.0	35.0	8.0	37.0
49	27.789720106847177	32.0	24.0	35.0	8.0	37.0
50	29.54922657464094	34.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	7.0
11	15.0
12	42.0
13	97.0
14	255.0
15	578.0
16	1127.0
17	2154.0
18	3733.0
19	5989.0
20	8905.0
21	12597.0
22	17396.0
23	23071.0
24	30342.0
25	37825.0
26	45192.0
27	49437.0
28	55541.0
29	65331.0
30	78548.0
31	92403.0
32	99269.0
33	96754.0
34	80439.0
35	60263.0
36	30243.0
37	6370.0
38	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.37764283620637	23.32719459791283	26.83833004274993	24.456832523130867
2	37.74138779663642	17.389433632527552	31.137878209701412	13.731300361134616
3	28.329987446009547	15.001631465719864	42.237596712734835	14.430784375535758
4	25.165165165165167	25.649295704544322	35.6727998716949	13.512739258595612
5	19.393869007128675	29.74211780841615	38.238127630392825	12.62588555406235
6	18.22739866938762	35.427471670565595	35.81083846277217	10.534291197274623
7	79.0562938629237	3.293348597216	16.1396755871894	1.510681952670903
8	81.42850032352796	1.6765937207926158	15.584534811054148	1.3103711446252881
9	69.45365254757519	2.9095393736277715	15.630879498282813	12.005928580514217
10	48.8270591033022	21.078426492791134	21.38060712646348	8.71390727744319
11	43.55371946532168	17.277498492968107	29.07526310841228	10.093518933297938
12	39.25605163174224	19.169003257401048	30.286197800009955	11.288747310846759
13	19.133608746868415	37.67867314828641	31.007913991339404	12.17980411350577
14	13.095194642155969	37.2208672761159	36.161354724890636	13.522583356837501
15	11.531420923686117	18.469519242999908	54.00759875897998	15.991461074334001
16	13.223499742836758	14.717811734386322	51.313634076065014	20.745054446711904
17	15.003511799091909	16.020108506296353	36.84103993496259	32.13533975964915
18	19.716733307893527	19.693948091738147	42.479385462810875	18.109933137557448
19	27.241495639285695	20.83409376227056	33.74346722413021	18.18094337431354
20	28.66247462932546	20.697493073183683	33.62047130002931	17.01956099746155
21	19.700142131081357	25.29037324617435	36.45358065247568	18.555903970268613
22	20.193895553011576	21.23825482941505	33.802421205736124	24.765428411837252
23	16.812834934381897	27.36106272017874	33.78881644075014	22.03728590468922
24	16.804981777357465	20.290788025594654	48.0545739109275	14.849656286120375
25	15.136794252816351	21.50293940349189	44.26183089166514	19.098435452026614
26	11.631631631631631	42.6942965064512	29.44402966502414	16.230042196893027
27	14.990349465487588	31.238752564719412	36.741935305471216	17.02896266432178
28	12.875748676853648	25.47342923033531	46.049253673563065	15.601568419247977
29	13.645358065247567	21.722053545257967	45.266924382946485	19.365664006547984
30	17.31344604272781	27.234859168560828	38.233703316576246	17.217991472135118
31	27.195703991284105	23.08197700462894	33.181800585115504	16.54051841897146
32	28.20599605129992	22.615433112670683	33.492166199348524	15.686404636680878
33	26.589241174876534	24.45639009174921	31.45687123587676	17.4974974974975
34	17.418634103716975	24.383610129466483	34.460537885952256	23.73721788086429
35	18.773248386508055	24.393122404172125	36.21942384373323	20.614205365586578
36	29.356207035765046	23.11958367206986	29.43341131186435	18.090797980300742
37	18.328826063632693	31.819333145299993	31.31839021341784	18.533450577649475
38	19.51586946062084	31.329672213650113	28.532842234499693	20.62161609122935
39	19.77214783844618	29.88502314469165	29.84166486928918	20.501164147572986
40	24.072359652470148	24.085079554692815	28.503973586846516	23.33858720599052
41	16.757641619520072	22.848262627268152	30.54413529551651	29.849960457695264
42	20.446745087629065	22.900801353840027	27.538145880687313	29.114307677843588
43	20.734878524933773	23.83455278482903	29.161647835680988	26.268920854556217
44	18.15307019726909	27.855258573490616	31.0621118355925	22.929559393647793
45	16.138127077353598	35.93460864179096	25.481171779514323	22.44609250134112
46	19.68609493471372	30.334754644146912	28.453315193646684	21.52583522749269
47	19.903439350953164	24.817635314872884	30.1306831693572	25.148242164816754
48	20.94757741166581	22.009080904108526	34.75198955861939	22.29135212560627
49	20.34476465415692	23.169578418197204	33.67898285025357	22.80667407739231
50	18.13271835371283	31.67410504427079	29.42301417439539	20.770162427620992
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	260.0
1	471.0
2	682.0
3	8703.5
4	16725.0
5	13421.0
6	10117.0
7	10253.0
8	10389.0
9	11349.0
10	12309.0
11	12807.0
12	13305.0
13	13299.5
14	13294.0
15	12678.5
16	12063.0
17	11386.0
18	10709.0
19	10070.0
20	9431.0
21	8766.5
22	8102.0
23	8133.5
24	8165.0
25	7694.5
26	7224.0
27	7777.0
28	8330.0
29	9634.0
30	10938.0
31	12912.5
32	14887.0
33	16698.5
34	18510.0
35	19951.0
36	21392.0
37	23763.0
38	26134.0
39	32536.5
40	38939.0
41	51189.5
42	63440.0
43	76555.5
44	89671.0
45	89280.5
46	88890.0
47	82420.5
48	75951.0
49	69429.0
50	62907.0
51	58678.0
52	54449.0
53	49782.5
54	45116.0
55	40414.5
56	35713.0
57	32382.0
58	29051.0
59	26863.5
60	24676.0
61	21819.0
62	18962.0
63	16735.0
64	14508.0
65	12035.0
66	9562.0
67	7932.0
68	6302.0
69	5437.5
70	4573.0
71	3894.5
72	3216.0
73	2717.5
74	2219.0
75	1734.0
76	1249.0
77	964.5
78	680.0
79	550.0
80	420.0
81	357.0
82	294.0
83	235.5
84	177.0
85	131.5
86	86.0
87	64.0
88	42.0
89	30.0
90	18.0
91	14.5
92	11.0
93	6.0
94	1.0
95	2.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	904095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.928070419785826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.93958961129104	33.53629293779271
2	7.007333380758143	5.735932681251703
3	2.7060355430411533	3.322584397920951
4	1.5033184967988422	2.4611170120139834
5	1.016510580942513	2.080190831963628
6	0.7047608231772032	1.7306700360061675
7	0.5470905754716088	1.567395311923221
8	0.4315820399086311	1.4131056097036212
9	0.353296109891516	1.301375525820872
>10	3.25016428796581	28.50347777174428
>50	0.45872187601552716	12.786279447027118
>100	0.08100324437635804	4.318884982150198
>500	2.9671518086538714E-4	0.07772147082929952
>1k	0.0	0.0
>5k	2.9671518086538714E-4	1.1649719838522972
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9593	1.0610610610610611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023448863227868752	0.0
2	0.0	0.0	0.0	0.08074372715256693	0.0
3	0.0	0.0	0.0	0.11027602187823182	0.0
4	0.0	0.0	0.0	0.16480568966756812	0.0
5	0.0	0.0	0.0	0.2672285545213722	0.0
6	0.0	0.0	0.0	0.3900032629314397	0.0
7	0.0	0.0	0.0	0.46809240179405925	0.0
8	0.0	0.0	0.0	0.7160751912133128	0.0
9	0.0	0.0	0.0	0.8648427432957819	0.0
10	0.0	0.0	0.0	1.0672551004042716	0.0
11	0.0	0.0	0.0	1.220004534921662	0.0
12	0.0	0.0	0.0	1.3556097533998086	0.0
13	0.0	0.0	0.0	1.4129046173245068	0.0
14	0.0	0.0	0.0	1.4321503824266255	0.0
15	0.0	0.0	0.0	1.4593599123985865	0.0
16	0.0	0.0	0.0	1.5112349918979753	0.0
17	0.0	0.0	0.0	1.5965136407125358	0.0
18	0.0	0.0	0.0	1.7108821528711031	0.0
19	0.0	0.0	0.0	1.7513646242928012	0.0
20	0.0	0.0	0.0	1.8030184881013611	0.0
21	0.0	0.0	0.0	1.8545617440645066	0.0
22	0.0	0.0	0.0	1.9119672158346193	0.0
23	0.0	0.0	0.0	1.9736863935758964	0.0
24	0.0	0.0	0.0	2.0216901984857785	0.0
25	0.0	0.0	0.0	2.0584120031633844	0.0
26	0.0	0.0	0.0	2.09834143535801	0.0
27	0.0	0.0	0.0	2.1566317698914386	0.0
28	0.0	0.0	0.0	2.2071795552458537	0.0
29	0.0	0.0	0.0	2.275203380175756	0.0
30	0.0	0.0	0.0	2.359928989763244	0.0
31	0.0	0.0	0.0	2.431381657900995	0.0
32	0.0	0.0	0.0	2.4978569729950943	0.0
33	0.0	0.0	0.0	2.566876268533727	0.0
34	0.0	0.0	0.0	2.6460714858504915	0.0
35	0.0	0.0	0.0	2.7362168798632887	0.0
36	0.0	0.0	0.0	2.8092180578368424	0.0
37	0.0	0.0	0.0	2.8913996869797973	0.0
38	0.0	0.0	0.0	2.9921634341523844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	20	7.8555883E-4	44.000004	6
TCGTTGC	20	7.8555883E-4	44.000004	28
ACCGGTA	20	7.8555883E-4	44.000004	40
CGAACGG	20	7.8555883E-4	44.000004	2
AGTCACG	20	7.8555883E-4	44.000004	1
TAGCACG	20	7.8555883E-4	44.000004	1
GGAACGT	20	7.8555883E-4	44.000004	8
TCGTCGG	20	7.8555883E-4	44.000004	2
TCGGTCA	20	7.8555883E-4	44.000004	16
TACGTTG	40	8.309144E-9	44.000004	1
TAGTACG	20	7.8555883E-4	44.000004	1
TCGTTCA	35	1.4456236E-7	44.0	16
CGAAAGG	75	0.0	44.0	2
TAACGGC	55	1.8189894E-12	44.0	3
ATATCGA	35	1.4456236E-7	44.0	18
CCGAACT	25	4.4420834E-5	44.0	12
CCGTTTG	30	2.5271147E-6	44.0	1
ATACCGG	50	2.7284841E-11	44.0	2
ATCGACG	30	2.5271147E-6	44.0	1
ACGAACT	35	1.4456236E-7	44.0	1
>>END_MODULE