FastQCFastQC Report
Thu 26 May 2016
SRR1545952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545952_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1029502
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86920.8442917060870208No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31770.30859580651616025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAC950.044.0000046
TCACGAC1350.044.025
ATTTATA254.4425164E-544.035
ATCCTAC302.5274585E-644.029
TATCACG254.4425164E-544.01
GTGCACG351.4458965E-744.01
GTGCAAG1000.044.01
AACCGAG302.5274585E-644.01
ATCTCGC207.8561E-444.011
GGTACGC302.5274585E-644.07
AAATCGT454.802132E-1044.012
CGAACGT254.4425164E-544.038
TCACCCG207.8561E-444.038
GTCGAAT207.8561E-444.030
GTACACG207.8561E-444.023
GATCGAC408.310963E-944.09
GGTCGAC207.8561E-444.09
ACTTGTC302.5274585E-644.010
CCGATCA302.5274585E-644.012
TCTTCGA254.4425164E-544.028
Produced by FastQC (version 0.11.5)