##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545952_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1029502
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.555947438664518	31.0	31.0	33.0	30.0	34.0
2	31.949843710842718	31.0	31.0	34.0	30.0	34.0
3	31.995356978422578	33.0	31.0	34.0	30.0	34.0
4	35.751739190404685	37.0	35.0	37.0	35.0	37.0
5	35.68032116499045	37.0	35.0	37.0	33.0	37.0
6	35.74332638499003	37.0	35.0	37.0	33.0	37.0
7	36.005437580500086	37.0	35.0	37.0	35.0	37.0
8	36.00535695899571	37.0	35.0	37.0	35.0	37.0
9	37.852497615351886	39.0	38.0	39.0	35.0	39.0
10	37.36354373279508	39.0	37.0	39.0	35.0	39.0
11	36.93946004961622	39.0	35.0	39.0	33.0	39.0
12	35.58935582446659	35.0	35.0	39.0	32.0	39.0
13	35.00429139525713	35.0	35.0	39.0	30.0	39.0
14	35.853963372582086	36.0	35.0	40.0	31.0	41.0
15	36.24132833156225	36.0	35.0	40.0	31.0	41.0
16	36.54291880928837	36.0	35.0	41.0	32.0	41.0
17	36.49849053231562	36.0	35.0	40.0	32.0	41.0
18	36.44338233437138	36.0	35.0	40.0	32.0	41.0
19	36.24070472908261	36.0	35.0	40.0	32.0	41.0
20	35.968607151807376	35.0	35.0	40.0	32.0	41.0
21	35.76571002290428	35.0	35.0	40.0	31.0	41.0
22	35.6437889387296	35.0	35.0	40.0	31.0	41.0
23	35.662424162361994	35.0	35.0	40.0	31.0	41.0
24	35.677320685146796	35.0	35.0	40.0	31.0	41.0
25	35.635893859361126	35.0	35.0	40.0	31.0	41.0
26	35.397913748589126	35.0	35.0	40.0	31.0	41.0
27	35.34767100986691	35.0	34.0	40.0	31.0	41.0
28	35.39546402046815	35.0	35.0	40.0	31.0	41.0
29	35.42201180765069	35.0	35.0	40.0	31.0	41.0
30	35.31912808328687	36.0	35.0	40.0	30.0	41.0
31	34.988404102177554	35.0	34.0	40.0	30.0	41.0
32	34.668697098208646	35.0	34.0	40.0	29.0	41.0
33	34.3435136600026	35.0	34.0	40.0	27.0	41.0
34	34.11174723312825	35.0	34.0	40.0	24.0	41.0
35	33.87005659046801	35.0	33.0	40.0	23.0	41.0
36	33.60635142039549	35.0	33.0	40.0	22.0	41.0
37	33.489513376370326	35.0	33.0	39.0	22.0	41.0
38	33.48633416933624	35.0	33.0	39.0	23.0	41.0
39	33.45165720901951	35.0	33.0	39.0	23.0	41.0
40	33.22369941971944	35.0	33.0	39.0	21.0	41.0
41	33.2698236623144	35.0	33.0	39.0	21.0	41.0
42	33.21148963285161	35.0	33.0	39.0	21.0	41.0
43	33.121829777892614	35.0	33.0	39.0	21.0	41.0
44	32.99210589197496	35.0	33.0	39.0	20.0	41.0
45	32.86773993639643	35.0	33.0	38.0	20.0	41.0
46	32.870260572587526	35.0	33.0	38.0	20.0	41.0
47	32.841155238163694	35.0	33.0	38.0	20.0	41.0
48	32.806760938783995	35.0	33.0	38.0	20.0	41.0
49	32.822763821731286	35.0	33.0	38.0	20.0	41.0
50	32.59035242282191	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	1.0
14	11.0
15	42.0
16	79.0
17	197.0
18	494.0
19	1072.0
20	1873.0
21	3051.0
22	4758.0
23	6907.0
24	10169.0
25	15411.0
26	22557.0
27	27229.0
28	27831.0
29	26852.0
30	28990.0
31	33504.0
32	41790.0
33	56685.0
34	116943.0
35	226357.0
36	52063.0
37	62515.0
38	94903.0
39	167215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.523882420820943	22.5608109551997	28.872600538901334	17.042706085078027
2	33.47705978230251	23.660177445017105	30.53320926039969	12.329553512280695
3	26.56789399146383	23.537691038968354	37.001288001383195	12.893126968184617
4	23.881060940143875	25.86920666497005	35.61168409580554	14.638048299080525
5	22.273681838403423	30.979347296071303	34.22994807198043	12.517022793544841
6	19.760524991695014	40.323088250435646	29.830927963228827	10.085458794640516
7	84.89959223002967	4.052056236899006	9.823778875611703	1.224572657459626
8	87.87753690619347	1.7682335731256473	9.36005952392516	0.9941699967557129
9	85.8454864585013	2.7405483427909805	10.060300999900923	1.3536641988068017
10	61.89720855326167	16.792390884136214	13.936641210993276	7.373759351608836
11	57.230097658868075	14.31808777447737	18.76586932322618	9.685945243428376
12	50.398639342128526	18.17247562413672	21.40996326379162	10.018921769943137
13	19.64765488556603	47.947745609042045	22.31982065114978	10.084778854242147
14	12.205318688064715	48.17785686671808	27.999362798712387	11.617461646504815
15	10.273219478932532	19.428616942949116	58.88788948443033	11.410274093688017
16	11.647573292718228	12.585793908122566	57.218830075123705	18.547802724035506
17	12.461559083906588	14.263886811293228	30.488527462792693	42.7860266420075
18	20.131092508805228	20.396172129825878	39.427023939730084	20.045711421638813
19	29.796153868569462	20.156056034859574	28.23918749065082	21.808602605920143
20	33.41586514644945	18.99568917787435	26.739044703167163	20.849400972509038
21	20.279805187362435	28.44074125159543	31.151663619886122	20.127789941156017
22	21.8149163381907	21.87513963061752	27.26968961692158	29.0402544142702
23	17.471942745133084	30.18546831380609	26.001309370938568	26.341279570122257
24	17.979761088370882	20.183545053822137	46.16717597440316	15.669517883403822
25	14.510899444585828	21.452799508888766	43.14299535115036	20.893305695375044
26	14.181808291776024	35.71853187269184	29.995570673976353	20.104089161555784
27	15.296230604700137	35.99147937546503	31.182843743868393	17.529446275966436
28	11.734217126338754	27.379354289743972	45.81739520661446	15.069033377302812
29	12.704200671781113	21.401706844668585	43.66926921948671	22.224823264063595
30	16.39200312384046	30.732820334491823	34.89832948357555	17.976847058092165
31	30.893383402849146	24.60374044926576	26.43889958445928	18.06397656342581
32	32.5271830457833	24.846284902797663	27.37440043827015	15.252131613148881
33	30.050063040188363	27.123502431272595	25.526905241563398	17.299529286975645
34	18.388599536474917	27.208494981068515	29.133600517531775	25.26930496492479
35	18.854941515412307	24.734094737067046	33.90746205446906	22.503501693051593
36	34.67764025713403	21.370915258056808	26.714469714483315	17.236974770325848
37	19.59073416078842	32.029175271150514	30.649770471548383	17.730320096512685
38	19.685537279189354	33.42509290899872	24.51204562982879	22.377324181983134
39	19.709626596160085	32.165551888194486	28.8870735559523	19.237747959693134
40	24.205781047535606	25.71359744808655	25.80772062608912	24.272900878288723
41	16.116530128159052	22.729436173994806	28.209852919178395	32.94418077866774
42	22.837255294307347	23.452407086144564	23.547793010601243	30.162544608946853
43	21.775868332455886	25.918745179708246	25.12078655505283	27.184599932783033
44	18.86174091939598	30.81373324189754	28.752639625760807	21.571886212945675
45	15.464175883096875	41.796130556327235	21.337986715907302	21.401706844668585
46	22.0866982288524	34.04063323820644	25.727390524739146	18.145278008202023
47	21.763920808313145	26.961773750803786	26.652303735204015	24.622001705679057
48	22.758479342439355	22.085046945027788	33.16049895969119	21.995974752841665
49	21.03997855273715	21.401706844668585	34.611686038492394	22.946628564101868
50	18.68777331175656	34.30367303803198	26.257258363752573	20.75129528645889
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	286.0
1	722.0
2	1158.0
3	8272.0
4	15386.0
5	11712.0
6	8038.0
7	8534.5
8	9031.0
9	9674.5
10	10318.0
11	10274.0
12	10230.0
13	9467.5
14	8705.0
15	7733.5
16	6762.0
17	6037.0
18	5312.0
19	4837.5
20	4363.0
21	4127.5
22	3892.0
23	3582.0
24	3272.0
25	4322.5
26	5373.0
27	5123.0
28	4873.0
29	6505.0
30	8137.0
31	10893.0
32	13649.0
33	15318.5
34	16988.0
35	17970.5
36	18953.0
37	19029.5
38	19106.0
39	31480.5
40	43855.0
41	66691.0
42	89527.0
43	108649.0
44	127771.0
45	123442.0
46	119113.0
47	105140.5
48	91168.0
49	84847.5
50	78527.0
51	71314.5
52	64102.0
53	59811.0
54	55520.0
55	48986.0
56	42452.0
57	38015.5
58	33579.0
59	32502.5
60	31426.0
61	27709.5
62	23993.0
63	22232.0
64	20471.0
65	16762.5
66	13054.0
67	10472.0
68	7890.0
69	6175.5
70	4461.0
71	4216.0
72	3971.0
73	3003.5
74	2036.0
75	1578.0
76	1120.0
77	950.0
78	780.0
79	657.5
80	535.0
81	350.5
82	166.0
83	136.0
84	106.0
85	65.5
86	25.0
87	17.5
88	10.0
89	6.5
90	3.0
91	3.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1029502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.924202244773255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.14562107908199	13.902491046648926
2	6.5498967798757555	2.2170355557001273
3	1.960496067337654	0.9953949583111518
4	0.9347800251091646	0.632816247972869
5	0.5950243211920793	0.5035155976206834
6	0.4043968268673759	0.4106456211028814
7	0.3275166623228543	0.3880070762179566
8	0.24507743492304798	0.3318192059414336
9	0.22900877535576822	0.34882117469539786
>10	3.1137955114725395	13.560905659637468
>50	1.7677416632887542	21.937626870926145
>100	1.723171883304067	43.39161498208735
>500	0.0023153132459577657	0.21659414580520545
>1k	5.788283114894414E-4	0.31122550937274435
>5k	5.788283114894414E-4	0.8514863479596769
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8692	0.8442917060870208	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3177	0.30859580651616025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030985855297027107	0.0
2	0.0	0.0	0.0	0.11549273337982831	0.0
3	0.0	0.0	0.0	0.16114587441306574	0.0
4	9.713434262390942E-5	0.0	0.0	0.23671639297446725	0.0
5	9.713434262390942E-5	0.0	0.0	0.38601187758741606	0.0
6	9.713434262390942E-5	0.0	0.0	0.5226798976592566	0.0
7	9.713434262390942E-5	0.0	0.0	0.6009701778141276	0.0
8	9.713434262390942E-5	0.0	0.0	0.7827085328634621	0.0
9	9.713434262390942E-5	0.0	0.0	0.8419604818640469	0.0
10	9.713434262390942E-5	0.0	0.0	0.9609500515783359	0.0
11	9.713434262390942E-5	0.0	0.0	1.0930527575468527	0.0
12	9.713434262390942E-5	0.0	0.0	1.2081569535561854	0.0
13	9.713434262390942E-5	0.0	0.0	1.2576954682943793	0.0
14	9.713434262390942E-5	0.0	0.0	1.2738197691699482	0.0
15	9.713434262390942E-5	0.0	0.0	1.3046113557817274	0.0
16	9.713434262390942E-5	0.0	0.0	1.3847471884464528	0.0
17	9.713434262390942E-5	0.0	0.0	1.4689626635013822	0.0
18	9.713434262390942E-5	0.0	0.0	1.6244747460422613	0.0
19	9.713434262390942E-5	0.0	0.0	1.6731390516968399	0.0
20	9.713434262390942E-5	0.0	0.0	1.7263686714547422	0.0
21	9.713434262390942E-5	0.0	0.0	1.8100013404539281	0.0
22	9.713434262390942E-5	0.0	0.0	1.8866403367841929	0.0
23	9.713434262390942E-5	0.0	0.0	1.9909626207622715	0.0
24	9.713434262390942E-5	0.0	0.0	2.06099648179411	0.0
25	9.713434262390942E-5	0.0	0.0	2.113449026811021	0.0
26	9.713434262390942E-5	0.0	0.0	2.180180320193647	0.0
27	9.713434262390942E-5	0.0	0.0	2.2310787157285756	0.0
28	9.713434262390942E-5	0.0	0.0	2.2872223657651953	0.0
29	9.713434262390942E-5	0.0	0.0	2.3482227329330105	0.0
30	9.713434262390942E-5	0.0	0.0	2.4454542098995438	0.0
31	9.713434262390942E-5	0.0	0.0	2.5421028808103334	0.0
32	9.713434262390942E-5	0.0	0.0	2.614467966065146	0.0
33	9.713434262390942E-5	0.0	0.0	2.687707260403574	0.0
34	9.713434262390942E-5	0.0	0.0	2.7631806446223512	0.0
35	9.713434262390942E-5	0.0	0.0	2.8899409617465532	0.0
36	9.713434262390942E-5	0.0	0.0	2.9769733327375762	0.0
37	9.713434262390942E-5	0.0	0.0	3.065559853210581	0.0
38	9.713434262390942E-5	0.0	0.0	3.147541238385161	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGAAC	95	0.0	44.000004	6
TCACGAC	135	0.0	44.0	25
ATTTATA	25	4.4425164E-5	44.0	35
ATCCTAC	30	2.5274585E-6	44.0	29
TATCACG	25	4.4425164E-5	44.0	1
GTGCACG	35	1.4458965E-7	44.0	1
GTGCAAG	100	0.0	44.0	1
AACCGAG	30	2.5274585E-6	44.0	1
ATCTCGC	20	7.8561E-4	44.0	11
GGTACGC	30	2.5274585E-6	44.0	7
AAATCGT	45	4.802132E-10	44.0	12
CGAACGT	25	4.4425164E-5	44.0	38
TCACCCG	20	7.8561E-4	44.0	38
GTCGAAT	20	7.8561E-4	44.0	30
GTACACG	20	7.8561E-4	44.0	23
GATCGAC	40	8.310963E-9	44.0	9
GGTCGAC	20	7.8561E-4	44.0	9
ACTTGTC	30	2.5274585E-6	44.0	10
CCGATCA	30	2.5274585E-6	44.0	12
TCTTCGA	25	4.4425164E-5	44.0	28
>>END_MODULE