FastQCFastQC Report
Thu 26 May 2016
SRR1545951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545951_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences961662
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79400.8256539199843603No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17340.18031283340716384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG302.5272839E-644.0000041
ATTCACG302.5272839E-644.00000441
CCGCAAT302.5272839E-644.00000433
GTAGTAA302.5272839E-644.0000041
AACGTCA207.85584E-444.010
TACCCGC254.442296E-544.022
CTCGTAG207.85584E-444.01
CGTGCAT207.85584E-444.044
TTTCGCG254.442296E-544.01
GACGTAA207.85584E-444.020
ATTCGTT207.85584E-444.018
ATAGACG254.442296E-544.01
TGACGTT207.85584E-444.013
CCCGTAT207.85584E-444.039
GGTATCG351.445751E-744.01
GCGATAC254.442296E-544.09
ACGTATG254.442296E-544.01
TCATACG207.85584E-444.020
GTGACGT207.85584E-444.020
CAATCGT254.442296E-544.042
Produced by FastQC (version 0.11.5)