##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545951_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	961662
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.470556182941614	31.0	31.0	33.0	30.0	34.0
2	31.869553959707257	31.0	31.0	34.0	30.0	34.0
3	31.962467062231845	31.0	31.0	34.0	30.0	34.0
4	35.73940116173874	37.0	35.0	37.0	35.0	37.0
5	35.66786563262352	37.0	35.0	37.0	33.0	37.0
6	35.729386208459935	37.0	35.0	37.0	33.0	37.0
7	35.93911374266634	37.0	35.0	37.0	35.0	37.0
8	35.91962664636848	37.0	35.0	37.0	35.0	37.0
9	37.7568969138845	39.0	38.0	39.0	35.0	39.0
10	37.27363252369336	39.0	37.0	39.0	34.0	39.0
11	36.947135272060244	39.0	37.0	39.0	33.0	39.0
12	35.93436779242603	37.0	35.0	39.0	32.0	39.0
13	35.52525003587539	37.0	35.0	39.0	31.0	39.0
14	36.38065245377274	38.0	35.0	41.0	31.0	41.0
15	36.67154156034033	38.0	35.0	41.0	32.0	41.0
16	36.90421062701864	38.0	35.0	41.0	32.0	41.0
17	36.85093307211889	38.0	35.0	41.0	32.0	41.0
18	36.78767487953148	38.0	35.0	40.0	32.0	41.0
19	36.64580278725789	37.0	35.0	40.0	32.0	41.0
20	36.42609565523021	36.0	35.0	40.0	32.0	41.0
21	36.23659144273144	36.0	35.0	40.0	31.0	41.0
22	36.153957419550736	36.0	35.0	40.0	31.0	41.0
23	36.12899022733559	36.0	35.0	40.0	31.0	41.0
24	36.14346828719446	36.0	35.0	40.0	31.0	41.0
25	36.08887946076688	36.0	35.0	40.0	31.0	41.0
26	35.89236238928022	36.0	35.0	40.0	31.0	41.0
27	35.863692232821926	36.0	35.0	40.0	31.0	41.0
28	35.85927176076418	36.0	35.0	40.0	31.0	41.0
29	35.81598108275049	36.0	35.0	40.0	31.0	41.0
30	35.70615663299579	36.0	35.0	40.0	30.0	41.0
31	35.443636121631094	36.0	34.0	40.0	30.0	41.0
32	35.18214299826758	35.0	34.0	40.0	29.0	41.0
33	34.93630402365904	35.0	34.0	40.0	27.0	41.0
34	34.71612375242029	35.0	34.0	40.0	26.0	41.0
35	34.48035380414324	35.0	34.0	40.0	24.0	41.0
36	34.25071282841581	35.0	33.0	40.0	23.0	41.0
37	34.15429641599647	35.0	33.0	40.0	23.0	41.0
38	34.0881515542883	35.0	33.0	40.0	23.0	41.0
39	34.00651580284965	35.0	33.0	40.0	23.0	41.0
40	33.81283236729745	35.0	33.0	40.0	23.0	41.0
41	33.78852029091302	35.0	33.0	40.0	22.0	41.0
42	33.74550829709399	35.0	33.0	40.0	22.0	41.0
43	33.65365273869613	35.0	33.0	40.0	22.0	41.0
44	33.52642924437068	35.0	33.0	40.0	22.0	41.0
45	33.42512545988092	35.0	33.0	40.0	22.0	41.0
46	33.42294693977718	35.0	33.0	39.0	22.0	41.0
47	33.36709571554247	35.0	33.0	39.0	22.0	41.0
48	33.2741742940867	35.0	33.0	39.0	22.0	41.0
49	33.2514833694167	35.0	33.0	39.0	21.0	41.0
50	33.057451578621176	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	4.0
14	11.0
15	30.0
16	72.0
17	194.0
18	415.0
19	926.0
20	1631.0
21	2604.0
22	4107.0
23	6044.0
24	8844.0
25	13369.0
26	18828.0
27	22604.0
28	23592.0
29	23862.0
30	26392.0
31	30439.0
32	37614.0
33	49736.0
34	95232.0
35	167109.0
36	58239.0
37	74248.0
38	111472.0
39	184037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.04896938841298	22.63830743026136	27.678123914639446	15.634599266686216
2	33.76997323383892	24.187708363229493	29.34367792426029	12.6986404786713
3	28.608700354178495	23.874500604162378	34.55424047118426	12.962558570474867
4	25.006187205067892	26.68837907705618	33.94851829436954	14.35691542350639
5	22.04235999758751	31.61495411069586	32.89606951298897	13.446616378727661
6	19.530874673221984	41.66432696727124	28.901526731845493	9.903271627661278
7	86.55889491318155	3.5579028806378954	8.626003731040637	1.2571984751399141
8	88.78108940563317	1.9851049537155465	8.215672450403572	1.0181331902477169
9	85.74311972397787	3.175855966025485	9.28611092046894	1.7949133895277136
10	55.47292083913059	21.108767945494364	14.300346691457081	9.117964523917967
11	49.18495271727488	18.76033367232978	19.831084102314534	12.223629508080801
12	43.73334913930258	19.728553275475168	23.542471263292093	12.995626321930159
13	21.74329442153272	39.11727821209531	25.206361486676194	13.933065879695777
14	15.522917615544754	41.183388758212345	29.051475466432073	14.242218159810827
15	14.205614862602452	21.768355201723683	49.43680835886205	14.589221576811811
16	17.18400019965435	15.858378515528324	47.811393192202665	19.146228092614663
17	17.52871591057981	16.972075427749044	29.673939492254036	35.82526916941711
18	23.043751338827985	20.950708253003654	35.803327988420044	20.20221241974831
19	29.466694119139575	22.598376560579496	27.20404882380712	20.730880496473812
20	32.30116194671309	21.215978171124576	25.83319295136961	20.649666930792733
21	22.45362715798274	26.848102555783633	30.0753279218686	20.622942364365027
22	23.021394211271737	21.341386058719173	27.88089786224266	27.75632186776643
23	19.41420166337029	28.11517976170422	27.408070611087886	25.062547963837606
24	20.46155509940083	22.20665888846601	39.96320952684	17.36857648529317
25	18.21689949275317	22.04059222471097	37.694325033119746	22.048183249416116
26	17.045178035525996	32.32913435281835	28.462287165344996	22.163400446310657
27	17.27415661635793	31.777797188617207	31.23550686207836	19.712539332946502
28	15.61359396544732	26.174997036380766	40.433125152080464	17.778283846091455
29	16.375712048516007	23.147426018705115	39.40292951161635	21.073932421162528
30	19.65170714866554	27.999858578169878	33.825398112850465	18.523036160314124
31	31.232699222803856	23.46073776441203	26.142137258205068	19.16442575457905
32	30.614810609132938	25.177661174092353	26.72456642770537	17.48296178906934
33	28.53861335895564	25.50605098256976	26.702417273428708	19.252918385045888
34	20.50481354155618	25.999051641845057	28.837262988451247	24.658871828147518
35	20.54401650475947	24.995788541088242	31.971420311918326	22.488774642233967
36	32.279636712275206	22.42555076523768	26.410942722079067	18.883869800408043
37	21.388388019907204	30.751968987024547	27.713999305369246	20.145643687699007
38	21.776154199708422	31.600084021204957	24.26299469044217	22.36076708864445
39	21.3705023178622	30.13012888104136	26.45056163184154	22.048807169254893
40	25.091976182900023	25.365876992124054	25.65059241188692	23.891554413089004
41	18.919745191137842	23.140042967279566	27.996946952255573	29.943264889327022
42	23.30600564439481	24.30812489211386	24.340464737090578	28.04540472640075
43	22.873733182760677	24.733014302322438	25.18400435911994	27.209248155796946
44	20.774762858467945	27.98550842187796	28.920140340369073	22.31958837928503
45	18.405219297424665	35.595666668746404	23.255572124093497	22.743541909735438
46	22.770786409362128	30.72524442059684	26.870667656619478	19.633301513421557
47	22.478895911453296	26.727374066979877	27.24782719916145	23.545902822405377
48	23.835297640959087	23.681085454140852	30.569264460902062	21.914352443997995
49	21.87275778807939	22.145514744265657	32.687160353637765	23.29456711401719
50	20.189838009612526	30.837446004937284	27.38945700256431	21.583258982885877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	750.5
2	1261.0
3	7700.5
4	14140.0
5	10389.5
6	6639.0
7	6810.0
8	6981.0
9	7185.0
10	7389.0
11	7276.5
12	7164.0
13	6636.0
14	6108.0
15	5462.5
16	4817.0
17	4417.0
18	4017.0
19	3770.5
20	3524.0
21	3443.5
22	3363.0
23	3259.5
24	3156.0
25	3301.5
26	3447.0
27	4093.5
28	4740.0
29	6243.5
30	7747.0
31	9508.0
32	11269.0
33	12563.0
34	13857.0
35	16089.0
36	18321.0
37	20313.0
38	22305.0
39	29556.5
40	36808.0
41	54497.5
42	72187.0
43	85169.0
44	98151.0
45	95442.0
46	92733.0
47	87097.0
48	81461.0
49	78622.0
50	75783.0
51	73403.0
52	71023.0
53	64793.0
54	58563.0
55	52076.0
56	45589.0
57	42268.0
58	38947.0
59	37191.5
60	35436.0
61	32430.5
62	29425.0
63	27513.0
64	25601.0
65	21783.5
66	17966.0
67	14895.0
68	11824.0
69	9446.0
70	7068.0
71	6467.0
72	5866.0
73	4468.5
74	3071.0
75	2370.5
76	1670.0
77	1312.0
78	954.0
79	716.5
80	479.0
81	393.5
82	308.0
83	232.0
84	156.0
85	104.0
86	52.0
87	38.5
88	25.0
89	22.5
90	20.0
91	13.5
92	7.0
93	5.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	961662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.13025566187798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82754468674936	15.271213387175045
2	6.327798047814413	2.421047888628442
3	2.2712818016646494	1.3035060463804677
4	1.2226835673225696	0.9356099694583102
5	0.7968192398642182	0.7621677887452885
6	0.5882337676381298	0.675183741832029
7	0.4620483590140698	0.6187372265263238
8	0.38794304677253655	0.5937159733605211
9	0.31776731419647675	0.5471072965410219
>10	4.709133075052776	22.998100781291832
>50	2.2147289626920914	30.57775783661786
>100	0.8690708587779086	21.860544658095833
>500	0.0038478785651140796	0.41800475758329697
>1k	5.4969693787344E-4	0.1823447168929401
>5k	5.4969693787344E-4	0.834957930870787
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7940	0.8256539199843603	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1734	0.18031283340716384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029948152261397454	0.0
2	0.0	0.0	0.0	0.09618764181178002	0.0
3	0.0	0.0	0.0	0.13934209732733538	0.0
4	0.0	0.0	0.0	0.20246198768382237	0.0
5	0.0	0.0	0.0	0.34159611173156473	0.0
6	0.0	0.0	0.0	0.45847709486285204	0.0
7	0.0	0.0	0.0	0.5309557828010257	0.0
8	0.0	0.0	0.0	0.7140762554826956	0.0
9	0.0	0.0	0.0	0.7854110903831075	0.0
10	0.0	0.0	0.0	0.9284967067431177	0.0
11	0.0	0.0	0.0	1.0800052409266458	0.0
12	0.0	0.0	0.0	1.2156038192213063	0.0
13	0.0	0.0	0.0	1.2709247115930544	0.0
14	0.0	0.0	0.0	1.2938017723482886	0.0
15	0.0	0.0	0.0	1.3299891229974772	0.0
16	0.0	0.0	0.0	1.4177538469857394	0.0
17	0.0	0.0	0.0	1.504270731296443	0.0
18	0.0	0.0	0.0	1.6548433857218026	0.0
19	0.0	0.0	0.0	1.7177553027986965	0.0
20	0.0	0.0	0.0	1.7873223648225676	0.0
21	0.0	0.0	0.0	1.8797664876016729	0.0
22	0.0	0.0	0.0	1.9674272249501383	0.0
23	0.0	0.0	0.0	2.073597584182384	0.0
24	0.0	0.0	0.0	2.1455563389215753	0.0
25	0.0	0.0	0.0	2.204412777046405	0.0
26	0.0	0.0	0.0	2.2717961196345495	0.0
27	0.0	0.0	0.0	2.3528017120360376	0.0
28	0.0	0.0	0.0	2.429231892286479	0.0
29	0.0	0.0	0.0	2.512213230844101	0.0
30	0.0	0.0	0.0	2.6241028552651557	0.0
31	0.0	0.0	0.0	2.719146644039174	0.0
32	0.0	0.0	0.0	2.7972406105263596	0.0
33	0.0	0.0	0.0	2.8920764260207847	0.0
34	0.0	0.0	0.0	2.990967720467274	0.0
35	1.0398663979651895E-4	0.0	0.0	3.118767300777196	0.0
36	1.0398663979651895E-4	0.0	0.0	3.2125632498736563	0.0
37	1.0398663979651895E-4	0.0	0.0	3.3138462370354658	0.0
38	1.0398663979651895E-4	0.0	0.0	3.4364464853555616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	30	2.5272839E-6	44.000004	1
ATTCACG	30	2.5272839E-6	44.000004	41
CCGCAAT	30	2.5272839E-6	44.000004	33
GTAGTAA	30	2.5272839E-6	44.000004	1
AACGTCA	20	7.85584E-4	44.0	10
TACCCGC	25	4.442296E-5	44.0	22
CTCGTAG	20	7.85584E-4	44.0	1
CGTGCAT	20	7.85584E-4	44.0	44
TTTCGCG	25	4.442296E-5	44.0	1
GACGTAA	20	7.85584E-4	44.0	20
ATTCGTT	20	7.85584E-4	44.0	18
ATAGACG	25	4.442296E-5	44.0	1
TGACGTT	20	7.85584E-4	44.0	13
CCCGTAT	20	7.85584E-4	44.0	39
GGTATCG	35	1.445751E-7	44.0	1
GCGATAC	25	4.442296E-5	44.0	9
ACGTATG	25	4.442296E-5	44.0	1
TCATACG	20	7.85584E-4	44.0	20
GTGACGT	20	7.85584E-4	44.0	20
CAATCGT	25	4.442296E-5	44.0	42
>>END_MODULE