FastQCFastQC Report
Thu 26 May 2016
SRR1545950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545950_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1355707
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113690.8386030314809911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC207.8569836E-444.09
ATGTCGC207.8569836E-444.033
CCGGATA351.4463512E-743.99999620
TGTAACG351.4463512E-743.9999961
CGTTTTT89450.043.06541
TCACGAC2050.041.8536625
ACGGGAT7150.041.230775
CGGTCTA2200.041.031
GCGTAAG700.040.857141
TACGGGA7400.040.729734
TGCGACG603.6379788E-1240.3333361
ATTACGG1650.040.02
CGAGGGA9000.039.8444444
CGACCCG1800.039.1111132
CACGACG2250.039.1111126
TTACGGG4700.038.8510633
CTAGACG404.125268E-738.51
TCGATAG404.125268E-738.51
ACGGGAC3600.038.55
GAACGTA404.125268E-738.59
Produced by FastQC (version 0.11.5)