FastQCFastQC Report
Thu 26 May 2016
SRR1545949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545949_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1477103
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121060.8195772400435177No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA62610.4238702378913319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT207.857211E-444.00000413
CGTGAGC207.857211E-444.00000418
CTATACG207.857211E-444.0000041
GACCGTA207.857211E-444.0000049
CGTTGCA207.857211E-444.00000438
ATCGTTA207.857211E-444.00000415
ATCCGTT207.857211E-444.00000438
ATCGTAG800.044.0000041
CGTAGGT207.857211E-444.00000435
ACTACGG207.857211E-444.0000042
ATGCGGT207.857211E-444.0000043
CGTCGAG207.857211E-444.00000442
CGCACGA207.857211E-444.00000440
CGATATC408.314601E-944.00000410
AATGTCG207.857211E-444.0000042
CGCTTAT207.857211E-444.00000444
ACGGTAC950.044.00000413
TCACGTC408.314601E-944.00000438
AACGTAG254.4434593E-544.01
AACCGGT351.4464604E-744.022
Produced by FastQC (version 0.11.5)