##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545949_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1477103
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522976393657043	31.0	31.0	33.0	30.0	34.0
2	31.925877883939034	31.0	31.0	34.0	30.0	34.0
3	31.98928578440366	33.0	31.0	34.0	30.0	34.0
4	35.75210530342163	37.0	35.0	37.0	35.0	37.0
5	35.673858898126944	37.0	35.0	37.0	33.0	37.0
6	35.72058075841698	37.0	35.0	37.0	33.0	37.0
7	35.97936704481678	37.0	35.0	37.0	35.0	37.0
8	35.97049359455637	37.0	35.0	37.0	35.0	37.0
9	37.77457496193563	39.0	38.0	39.0	35.0	39.0
10	37.31007654848714	39.0	37.0	39.0	34.0	39.0
11	36.96391585420922	39.0	37.0	39.0	33.0	39.0
12	35.81485380504948	37.0	35.0	39.0	32.0	39.0
13	35.337017797675585	37.0	35.0	39.0	31.0	39.0
14	36.25446769791951	38.0	35.0	41.0	31.0	41.0
15	36.5335992141374	38.0	35.0	41.0	32.0	41.0
16	36.77235643012031	38.0	35.0	41.0	32.0	41.0
17	36.72973381003221	38.0	35.0	41.0	32.0	41.0
18	36.65051861650812	37.0	35.0	40.0	32.0	41.0
19	36.50699578837766	37.0	35.0	40.0	32.0	41.0
20	36.28357128785196	36.0	35.0	40.0	32.0	41.0
21	36.10697832175549	35.0	35.0	40.0	31.0	41.0
22	35.989538982724966	35.0	35.0	40.0	31.0	41.0
23	35.95908206807515	35.0	35.0	40.0	31.0	41.0
24	35.98035411206937	35.0	35.0	40.0	31.0	41.0
25	35.93539990102247	35.0	35.0	40.0	31.0	41.0
26	35.71338288528288	35.0	35.0	40.0	31.0	41.0
27	35.679604604418245	35.0	35.0	40.0	31.0	41.0
28	35.68483240505232	36.0	35.0	40.0	31.0	41.0
29	35.66920587122225	36.0	35.0	40.0	31.0	41.0
30	35.58528010572046	36.0	35.0	40.0	30.0	41.0
31	35.340143510642115	35.0	34.0	40.0	30.0	41.0
32	35.07720043896736	35.0	34.0	40.0	29.0	41.0
33	34.811744340103566	35.0	34.0	40.0	27.0	41.0
34	34.56877888678041	35.0	34.0	40.0	26.0	41.0
35	34.34842729315424	35.0	34.0	40.0	24.0	41.0
36	34.13424182335287	35.0	33.0	40.0	23.0	41.0
37	34.042016704319195	35.0	33.0	40.0	23.0	41.0
38	33.978959490299594	35.0	33.0	40.0	23.0	41.0
39	33.91637617688137	35.0	33.0	40.0	23.0	41.0
40	33.73229422728137	35.0	33.0	40.0	23.0	41.0
41	33.75177898900754	35.0	33.0	40.0	23.0	41.0
42	33.708389326946055	35.0	33.0	40.0	23.0	41.0
43	33.59467213863894	35.0	33.0	40.0	23.0	41.0
44	33.48412399135334	35.0	33.0	39.0	22.0	41.0
45	33.36037365031416	35.0	33.0	39.0	22.0	41.0
46	33.354644869044336	35.0	33.0	39.0	23.0	41.0
47	33.31699278926385	35.0	33.0	39.0	22.0	41.0
48	33.24264455491594	35.0	33.0	39.0	22.0	41.0
49	33.18645348360947	35.0	33.0	39.0	21.0	41.0
50	32.97177380318096	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	2.0
14	16.0
15	38.0
16	106.0
17	330.0
18	736.0
19	1476.0
20	2528.0
21	4074.0
22	6361.0
23	9139.0
24	13632.0
25	19897.0
26	28832.0
27	35175.0
28	36732.0
29	37187.0
30	40712.0
31	46650.0
32	58114.0
33	78125.0
34	155697.0
35	282243.0
36	83257.0
37	103407.0
38	159075.0
39	273559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.85180518894079	22.560579729375675	28.216177206328872	16.371437875354662
2	33.041094629149086	24.43600750929353	30.372560342779074	12.150337518778311
3	28.823514677040123	24.208873721060755	34.67354680073089	12.294064801168233
4	25.329648643324127	26.155928191872874	33.64877060028989	14.865652564513104
5	22.04280947232522	32.00101820929211	32.21481508060034	13.741357237782333
6	19.59470666568276	39.86871599340059	30.35360431872388	10.182973022192765
7	85.11193870705021	4.386762466801571	9.106744756459095	1.3945540696891146
8	87.78886780407325	2.60611480715969	8.410990973547545	1.1940264152195208
9	84.41706502525552	4.041492028653384	9.605017388767067	1.9364255573240323
10	56.72583428508371	20.533977657617648	13.780826387868686	8.95936166942996
11	52.57900092275217	17.26298030672201	19.00470041696483	11.15331835356099
12	46.670272824576216	18.79293454823394	23.407033903526024	11.12975872366382
13	21.581704187182613	42.407063014562965	23.46891178204905	12.542321016205369
14	14.529724738220692	44.738247772836424	27.729955189313138	13.002072299629749
15	13.0872390077063	20.99494754258843	53.35680720978835	12.56100623991692
16	15.475494938403077	14.769518442518903	51.955821631937646	17.79916498714037
17	15.85705262259978	15.457080515035173	30.371138640974937	38.31472822139011
18	21.414417274895523	20.517188036311616	36.89092771458727	21.17746697420559
19	29.87198590755012	22.177532643288924	27.20114981825912	20.74933163090184
20	32.34845505018946	20.917295544048045	25.956686839035598	20.7775625667269
21	22.13115808443961	26.66009073165514	29.643159617169555	21.565591566735698
22	22.30656900703607	23.459907670622833	26.62894869213589	27.604574630205203
23	19.541088197640924	28.92926221123375	26.322131902785383	25.207517688339948
24	19.680076474017046	22.146729104199235	41.054009097537545	17.11918532424618
25	16.816227439792623	22.26852155875386	39.36069454872138	21.554556452732136
26	16.245718815817177	32.70584380371579	29.39740830531114	21.651029075155897
27	16.897806043315867	33.7212096922151	31.405595953701265	17.975388310767766
28	14.30272635015974	27.017479485181468	41.919351595657176	16.760442569001622
29	15.09603595686963	23.03691753384835	40.41194148275375	21.45510502652828
30	18.026704975888613	29.394768001960596	34.43165439376942	18.146872628381367
31	30.6516200969059	23.99981585576632	26.252468514382542	19.09609553294523
32	31.1102881789557	25.33655405208709	27.523402227197423	16.029755541759783
33	29.296805977646784	25.657249359049437	25.58142526282866	19.46451940047512
34	19.436085364392326	26.923037865335054	29.421780336239244	24.219096434033375
35	20.15729437960657	24.265741793226336	32.72507062811463	22.85189319905247
36	32.472143107149606	22.678039378431972	26.664694337497114	18.185123176921312
37	21.146528034944076	31.694878420800716	28.85851562145632	18.300077922798884
38	21.795974959092224	32.38122189177058	23.319700792700306	22.50310235643689
39	21.33845777850292	30.350016214170576	28.11848598235871	20.193040024967793
40	24.295597531113266	25.565786542983123	25.77694311094081	24.3616728149628
41	17.079783874245734	24.179356483603378	27.66895741190696	31.071902230243932
42	22.67519597482369	24.217200831627856	24.165681066249274	28.94192212729918
43	22.78222981064963	25.840242691267974	25.07502862021132	26.302498877871077
44	19.99535577410648	29.64180561545133	28.396462535110956	21.96637607533124
45	17.035508018059677	37.82363179818875	22.98404376675154	22.156816417000034
46	22.46613810952926	32.171757825960675	25.858927914979525	19.503176149530535
47	22.216460192687983	26.641540908115413	27.315833763793044	23.826165135403556
48	24.28991072389671	22.725767938999514	31.161537143990635	21.82278419311314
49	21.6219857382999	22.335544643806152	32.753369264025594	23.28910035386835
50	19.761181176938912	32.15205710096046	26.86420649067804	21.22255523142259
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	1220.5
2	2020.0
3	12038.0
4	22056.0
5	16153.0
6	10250.0
7	10544.0
8	10838.0
9	11388.5
10	11939.0
11	11832.5
12	11726.0
13	10934.5
14	10143.0
15	9278.0
16	8413.0
17	7648.5
18	6884.0
19	6344.0
20	5804.0
21	5274.5
22	4745.0
23	5396.5
24	6048.0
25	6580.5
26	7113.0
27	8414.5
28	9716.0
29	11669.5
30	13623.0
31	14886.5
32	16150.0
33	18271.5
34	20393.0
35	22750.0
36	25107.0
37	28620.0
38	32133.0
39	45647.5
40	59162.0
41	89148.5
42	119135.0
43	138529.5
44	157924.0
45	158179.5
46	158435.0
47	146175.5
48	133916.0
49	124356.0
50	114796.0
51	109540.5
52	104285.0
53	92032.5
54	79780.0
55	73633.5
56	67487.0
57	62148.0
58	56809.0
59	55964.0
60	55119.0
61	49861.0
62	44603.0
63	37487.0
64	30371.0
65	26143.5
66	21916.0
67	17735.0
68	13554.0
69	11498.5
70	9443.0
71	7796.0
72	6149.0
73	5211.0
74	4273.0
75	3317.0
76	2361.0
77	1644.5
78	928.0
79	658.0
80	388.0
81	410.0
82	432.0
83	333.0
84	234.0
85	135.5
86	37.0
87	34.5
88	32.0
89	19.5
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1477103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.664701435554456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39383158506283	13.564039020608739
2	6.714992581798963	2.2380669303528538
3	2.21312576194747	1.1064324018656562
4	1.1452556917099954	0.7634137667886588
5	0.7336258221053311	0.6112827645399264
6	0.5454729260265618	0.5454086072046559
7	0.427932359632744	0.4991955505524396
8	0.32706685439272726	0.436037718233661
9	0.2869385642940982	0.43035709538770134
>10	3.0955141348286523	12.376613361792835
>50	1.3493183369077062	16.29960355180613
>100	1.7595341061677872	49.15631147199975
>500	0.006570022333415309	0.7163901407122528
>1k	0.0	0.0
>5k	4.1062639583845683E-4	0.4284381193045577
>10k+	4.1062639583845683E-4	0.8284094988501798
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12106	0.8195772400435177	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6261	0.4238702378913319	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03263144140929915	0.0
2	0.0	0.0	0.0	0.11163744166791348	0.0
3	0.0	0.0	0.0	0.15665799879900047	0.0
4	0.0	0.0	0.0	0.2236810838512954	0.0
5	0.0	0.0	0.0	0.3609768580796329	0.0
6	0.0	0.0	0.0	0.48899772053810736	0.0
7	0.0	0.0	0.0	0.571524125264115	0.0
8	0.0	0.0	0.0	0.7941897078267393	0.0
9	0.0	0.0	0.0	0.8913393311096112	0.0
10	0.0	0.0	0.0	1.0695936573143512	0.0
11	0.0	0.0	0.0	1.2042491281921437	0.0
12	0.0	0.0	0.0	1.343508204911912	0.0
13	0.0	0.0	0.0	1.422446505084615	0.0
14	0.0	0.0	0.0	1.4581244503599275	0.0
15	6.770008591140903E-5	0.0	0.0	1.4951563973534683	0.0
16	6.770008591140903E-5	0.0	0.0	1.5709804935742464	0.0
17	6.770008591140903E-5	0.0	0.0	1.6568919025958244	0.0
18	6.770008591140903E-5	0.0	0.0	1.8224186126492194	0.0
19	6.770008591140903E-5	0.0	0.0	1.8786773840416005	0.0
20	6.770008591140903E-5	0.0	0.0	1.9437371666024643	0.0
21	6.770008591140903E-5	0.0	0.0	2.020305963768268	0.0
22	6.770008591140903E-5	0.0	0.0	2.095656159387666	0.0
23	6.770008591140903E-5	0.0	0.0	2.1759484612785975	0.0
24	6.770008591140903E-5	0.0	0.0	2.239315741691676	0.0
25	6.770008591140903E-5	0.0	0.0	2.290226206297056	0.0
26	6.770008591140903E-5	0.0	0.0	2.347026578376728	0.0
27	6.770008591140903E-5	0.0	0.0	2.4037592503704888	0.0
28	6.770008591140903E-5	0.0	0.0	2.462387524769769	0.0
29	6.770008591140903E-5	0.0	0.0	2.5202710982240237	0.0
30	6.770008591140903E-5	0.0	0.0	2.6038130042387024	0.0
31	6.770008591140903E-5	0.0	0.0	2.687151809995647	0.0
32	1.3540017182281806E-4	0.0	0.0	2.759726302092677	0.0
33	1.3540017182281806E-4	0.0	0.0	2.8289157898941375	0.0
34	1.3540017182281806E-4	0.0	0.0	2.9024380831939274	0.0
35	1.3540017182281806E-4	0.0	0.0	3.005951514552472	0.0
36	1.3540017182281806E-4	0.0	0.0	3.0867854171306943	0.0
37	1.3540017182281806E-4	0.0	0.0	3.1718844251213354	0.0
38	1.3540017182281806E-4	0.0	0.0	3.2528537278713805	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	20	7.857211E-4	44.000004	13
CGTGAGC	20	7.857211E-4	44.000004	18
CTATACG	20	7.857211E-4	44.000004	1
GACCGTA	20	7.857211E-4	44.000004	9
CGTTGCA	20	7.857211E-4	44.000004	38
ATCGTTA	20	7.857211E-4	44.000004	15
ATCCGTT	20	7.857211E-4	44.000004	38
ATCGTAG	80	0.0	44.000004	1
CGTAGGT	20	7.857211E-4	44.000004	35
ACTACGG	20	7.857211E-4	44.000004	2
ATGCGGT	20	7.857211E-4	44.000004	3
CGTCGAG	20	7.857211E-4	44.000004	42
CGCACGA	20	7.857211E-4	44.000004	40
CGATATC	40	8.314601E-9	44.000004	10
AATGTCG	20	7.857211E-4	44.000004	2
CGCTTAT	20	7.857211E-4	44.000004	44
ACGGTAC	95	0.0	44.000004	13
TCACGTC	40	8.314601E-9	44.000004	38
AACGTAG	25	4.4434593E-5	44.0	1
AACCGGT	35	1.4464604E-7	44.0	22
>>END_MODULE