FastQCFastQC Report
Thu 26 May 2016
SRR1545948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545948_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2180880
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176570.8096273064084223No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28640.1313231356149811No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC254.4441593E-544.014
CGGATAT207.8580395E-444.06
TTAACGC207.8580395E-444.040
TCGAGTA207.8580395E-444.043
CGTTTTT116700.043.1328161
TCACGAC4950.043.1111125
ACGGTCT5300.040.6792530
CACGACG5350.039.8878526
CGTAGAC501.3496901E-939.629
CGGTCTA5400.039.51851731
CTATCGT452.3526809E-839.11110719
TCCGATT404.126814E-738.539
TACGTTG404.126814E-738.51
CGTAAGG3950.038.4303782
TTAGGGA29850.037.9564484
CGTTAGG3900.037.7948722
AATGGGC4950.037.777784
TCTCACG5650.037.76991323
CGTTACA357.2932144E-637.71428741
TAGGGAC26250.037.7142875
Produced by FastQC (version 0.11.5)