##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545948_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2180880
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48206274531382	31.0	31.0	33.0	30.0	34.0
2	31.89012371152929	31.0	31.0	34.0	30.0	34.0
3	31.983717581893547	33.0	31.0	34.0	30.0	34.0
4	35.736111569641615	37.0	35.0	37.0	35.0	37.0
5	35.6522788965922	37.0	35.0	37.0	33.0	37.0
6	35.70739242874436	37.0	35.0	37.0	33.0	37.0
7	35.97881405671105	37.0	35.0	37.0	35.0	37.0
8	35.96536489857305	37.0	35.0	37.0	35.0	37.0
9	37.76801153662742	39.0	38.0	39.0	35.0	39.0
10	37.23708365430468	39.0	37.0	39.0	34.0	39.0
11	36.96808490150765	39.0	37.0	39.0	33.0	39.0
12	36.3694944242691	38.0	35.0	39.0	32.0	39.0
13	36.119327060636074	38.0	35.0	39.0	32.0	39.0
14	37.12636687942482	39.0	35.0	41.0	32.0	41.0
15	37.28887467444334	39.0	35.0	41.0	32.0	41.0
16	37.432539617035324	39.0	35.0	41.0	33.0	41.0
17	37.35714252962107	39.0	35.0	41.0	32.0	41.0
18	37.261801199515794	39.0	35.0	41.0	32.0	41.0
19	37.1725312717802	38.0	35.0	41.0	32.0	41.0
20	36.99210777300906	38.0	35.0	41.0	32.0	41.0
21	36.86310342613991	38.0	35.0	41.0	32.0	41.0
22	36.7810425149481	38.0	35.0	41.0	32.0	41.0
23	36.6867718535637	38.0	35.0	41.0	31.0	41.0
24	36.74173452918088	38.0	35.0	41.0	32.0	41.0
25	36.70239398774807	38.0	35.0	41.0	32.0	41.0
26	36.482494681046184	38.0	35.0	41.0	31.0	41.0
27	36.46342944132644	38.0	35.0	41.0	31.0	41.0
28	36.44328069403177	38.0	35.0	41.0	31.0	41.0
29	36.382312185906606	38.0	35.0	40.0	31.0	41.0
30	36.26706375408092	38.0	35.0	40.0	31.0	41.0
31	36.08632616191629	38.0	35.0	40.0	30.0	41.0
32	35.89707640952276	38.0	35.0	40.0	30.0	41.0
33	35.669770459630975	38.0	35.0	40.0	29.0	41.0
34	35.44916134771285	38.0	35.0	40.0	27.0	41.0
35	35.22067422324933	38.0	34.0	40.0	26.0	41.0
36	35.05628507758336	38.0	34.0	40.0	25.0	41.0
37	34.99166208136165	38.0	34.0	40.0	25.0	41.0
38	34.90691876673636	38.0	34.0	40.0	25.0	41.0
39	34.81527227541176	37.0	34.0	40.0	24.0	41.0
40	34.67480741718939	37.0	34.0	40.0	23.0	41.0
41	34.61935594805767	37.0	34.0	40.0	23.0	41.0
42	34.60836726459044	37.0	34.0	40.0	23.0	41.0
43	34.516150819852534	37.0	34.0	40.0	23.0	41.0
44	34.39022458823961	37.0	34.0	40.0	23.0	41.0
45	34.264625747404715	36.0	33.0	40.0	23.0	41.0
46	34.22482346575694	36.0	33.0	40.0	23.0	41.0
47	34.15340413044276	36.0	33.0	40.0	23.0	41.0
48	34.03638164410697	36.0	33.0	40.0	23.0	41.0
49	33.98572502842889	36.0	33.0	40.0	23.0	41.0
50	33.83911723707861	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	5.0
14	21.0
15	56.0
16	167.0
17	386.0
18	862.0
19	1757.0
20	3111.0
21	5011.0
22	7533.0
23	11426.0
24	16779.0
25	25500.0
26	36441.0
27	45369.0
28	48064.0
29	49418.0
30	54969.0
31	64069.0
32	78956.0
33	101912.0
34	174876.0
35	274488.0
36	147878.0
37	193457.0
38	301849.0
39	536512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.21209786875023	22.87915887164814	29.00851949671692	14.900223762884707
2	33.621015369942405	24.2274678111588	28.954504603646235	13.19701221525256
3	28.42430578482081	24.671875573163128	34.22476248120025	12.679056160815819
4	25.885514104398226	26.90056307545578	32.74274604746707	14.471176772678918
5	22.39426286636587	31.288424856021425	32.174672609221965	14.142639668390741
6	20.13815524008657	40.879232236528374	28.659348519863542	10.323264003521514
7	87.2888925571329	3.2076501228861742	8.173764718829096	1.3296926011518286
8	88.7197828399545	1.9918106452441218	7.887320714573934	1.4010858002274311
9	84.77444884633726	3.9176846043798834	9.228935108763435	2.078931440519423
10	42.73371299658854	31.766488756832107	14.530785737867284	10.96901250871208
11	37.61371556435934	21.68262352811709	25.47998972891677	15.223671178606802
12	34.92626829536701	21.238995267965226	28.749037085946956	15.08569935072081
13	22.886266094420602	31.78042808407615	29.61309196287737	15.720213858625876
14	16.528236308279226	34.21627966692344	32.247166281501045	17.008317743296285
15	15.75790506584498	23.3866145776017	44.0109497083746	16.844530648178715
16	19.50474120538498	20.196526172921022	40.9360441656579	19.362688456036096
17	19.950937236344963	20.21459227467811	31.04058911998826	28.793881368988668
18	22.391557536407323	22.817853343604416	35.00481457026521	19.785774549723047
19	26.453770954843915	24.483694655368478	28.60189464803199	20.46063974175562
20	27.95678808554345	23.410916694178496	28.448424489197023	20.183870731081033
21	23.53141850995928	25.566193463189173	31.334094493965736	19.568293532885807
22	22.85086753970874	22.209245809031216	29.547476248120024	25.392410403140016
23	19.88302886908037	27.21089651883643	29.52175268698874	23.384321925094458
24	20.25943655771982	24.05712372987051	36.98360294926818	18.699836763141484
25	20.217068339385936	24.92892777227541	34.279235904772385	20.574767983566268
26	19.2027530171307	29.525925314551927	29.277676901067455	21.99364476724992
27	18.644767249917464	29.436282601518656	31.298099849601996	20.620850298961887
28	17.470057958255385	25.91486005649096	36.731961410073	19.88312057518066
29	18.672141520853966	24.564258464473056	35.67935879094677	21.084241223726202
30	21.09226550750156	26.865164520743917	32.757785847914604	19.28478412383992
31	27.046421627966694	25.044431605590407	28.56764241957375	19.341504346869154
32	27.29288177249551	25.373197975129308	28.76393932724405	18.56998092513114
33	26.128351857965594	25.556151645207436	27.99461685191299	20.32087964491398
34	20.411531125050438	26.381644106965997	30.12146472983383	23.085360038149737
35	20.827830967315947	25.85305014489564	31.96535343531052	21.353765452477898
36	28.101867136201903	25.400526393015667	27.44548072337772	19.052125747404716
37	21.050814350170572	30.20954843916217	28.561314698653756	20.178322512013498
38	20.83209530097942	30.43111037746231	27.074254429404643	21.662539892153625
39	20.94599427753934	28.650132056784415	28.100216426396685	22.303657239279556
40	23.59520010271083	25.45697149774403	28.049457099886283	22.898371299658855
41	19.06744066615311	24.926314148417152	29.215179193719965	26.791065991709768
42	21.979246909504422	26.08928505924214	26.654378049227834	25.2770899820256
43	21.886577895161587	25.570366090752355	28.01401269212428	24.529043321961776
44	20.67894611349547	27.53604049741389	29.582783096731596	22.202230292359047
45	19.800768497120426	31.401406771578444	26.067596566523605	22.73022816477752
46	22.219058361762226	29.111872271743515	28.77819962583911	19.89086974065515
47	21.634707090715676	26.698488683467225	28.745002017534205	22.921802208282894
48	22.08507574923884	25.51483804702689	30.714573933458055	21.68551227027622
49	21.23959135761711	24.755694948827998	32.14097061736547	21.86374307618943
50	20.60283005025494	28.810480173141116	29.62074942225157	20.965940354352373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	576.0
1	1826.0
2	3076.0
3	18040.5
4	33005.0
5	23613.0
6	14221.0
7	14037.5
8	13854.0
9	13948.5
10	14043.0
11	13556.5
12	13070.0
13	12362.0
14	11654.0
15	10628.0
16	9602.0
17	9227.5
18	8853.0
19	8442.0
20	8031.0
21	8185.5
22	8340.0
23	9220.5
24	10101.0
25	12485.0
26	14869.0
27	17921.0
28	20973.0
29	26269.5
30	31566.0
31	36192.0
32	40818.0
33	48365.5
34	55913.0
35	61498.0
36	67083.0
37	73299.0
38	79515.0
39	92425.5
40	105336.0
41	129172.5
42	153009.0
43	167080.5
44	181152.0
45	182590.5
46	184029.0
47	177387.5
48	170746.0
49	172268.5
50	173791.0
51	163712.5
52	153634.0
53	137697.5
54	121761.0
55	113570.5
56	105380.0
57	99244.0
58	93108.0
59	86396.5
60	79685.0
61	71697.5
62	63710.0
63	56227.0
64	48744.0
65	40817.5
66	32891.0
67	26018.0
68	19145.0
69	16422.5
70	13700.0
71	11856.5
72	10013.0
73	7749.0
74	5485.0
75	4376.0
76	3267.0
77	2466.5
78	1666.0
79	1262.5
80	859.0
81	580.0
82	301.0
83	231.5
84	162.0
85	117.0
86	72.0
87	52.5
88	33.0
89	29.5
90	26.0
91	16.0
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2180880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.9675641759856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50154417863972	14.889830771223176
2	7.326642503866387	2.7793712377317905
3	2.694982635324011	1.533517682660249
4	1.482943322694547	1.1251129057023257
5	0.9748621140888776	0.9245379855858888
6	0.6836370322883553	0.7780157569805829
7	0.5601795201619109	0.7437668699120704
8	0.45085651732691057	0.6841319941247631
9	0.3889534710938575	0.663974993200059
>10	4.1469233951150954	18.718668741221343
>50	1.5831577683311844	21.6811795047467
>100	1.1994415262772133	33.78481716318973
>500	0.0046518450436586435	0.5742651256996236
>1k	9.793357986649775E-4	0.2988352000547532
>5k	0.0	0.0
>10k+	2.4483394966624437E-4	0.8199740679668652
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17657	0.8096273064084223	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2864	0.1313231356149811	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032005429001137155	0.0
2	0.0	0.0	0.0	0.12857195260628737	0.0
3	0.0	0.0	0.0	0.18630094273871098	0.0
4	0.0	0.0	0.0	0.2686988738490884	0.0
5	0.0	0.0	0.0	0.47366200799677194	0.0
6	0.0	0.0	0.0	0.6588624775320054	0.0
7	0.0	0.0	0.0	0.7856003081324969	0.0
8	0.0	0.0	0.0	1.1149627673232823	0.0
9	0.0	0.0	0.0	1.2471112578408716	0.0
10	0.0	0.0	0.0	1.4882065955027328	0.0
11	0.0	0.0	0.0	1.7097685338028685	0.0
12	0.0	0.0	0.0	1.908082975679542	0.0
13	0.0	0.0	0.0	1.9783757015516672	0.0
14	0.0	0.0	0.0	2.007446535343531	0.0
15	0.0	0.0	0.0	2.0533912915887167	0.0
16	0.0	0.0	0.0	2.1649059095411025	0.0
17	0.0	0.0	0.0	2.298384138512894	0.0
18	0.0	0.0	0.0	2.491333773522615	0.0
19	0.0	0.0	0.0	2.5728146436300943	0.0
20	0.0	0.0	0.0	2.67804739371263	0.0
21	0.0	0.0	0.0	2.801071127251385	0.0
22	0.0	0.0	0.0	2.934365944022596	0.0
23	0.0	0.0	0.0	3.0840761527456806	0.0
24	0.0	9.170610028979128E-5	0.0	3.196828803051979	0.0
25	0.0	9.170610028979128E-5	0.0	3.29096511499945	0.0
26	0.0	9.170610028979128E-5	0.0	3.3851014269469206	0.0
27	4.585305014489564E-5	9.170610028979128E-5	0.0	3.4836396317083014	0.0
28	4.585305014489564E-5	9.170610028979128E-5	0.0	3.594328894758079	0.0
29	4.585305014489564E-5	9.170610028979128E-5	0.0	3.713134147683504	0.0
30	4.585305014489564E-5	9.170610028979128E-5	0.0	3.8569751659880414	0.0
31	4.585305014489564E-5	9.170610028979128E-5	0.0	3.9889402443050512	0.0
32	4.585305014489564E-5	9.170610028979128E-5	0.0	4.106828436227578	0.0
33	4.585305014489564E-5	9.170610028979128E-5	0.0	4.232511646674737	0.0
34	4.585305014489564E-5	9.170610028979128E-5	0.0	4.362963574336965	0.0
35	4.585305014489564E-5	9.170610028979128E-5	0.0	4.5282179670591685	0.0
36	4.585305014489564E-5	9.170610028979128E-5	0.0	4.658761600821687	0.0
37	4.585305014489564E-5	9.170610028979128E-5	0.0	4.791185209640145	0.0
38	4.585305014489564E-5	9.170610028979128E-5	0.0	4.945755841678588	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	25	4.4441593E-5	44.0	14
CGGATAT	20	7.8580395E-4	44.0	6
TTAACGC	20	7.8580395E-4	44.0	40
TCGAGTA	20	7.8580395E-4	44.0	43
CGTTTTT	11670	0.0	43.132816	1
TCACGAC	495	0.0	43.11111	25
ACGGTCT	530	0.0	40.67925	30
CACGACG	535	0.0	39.88785	26
CGTAGAC	50	1.3496901E-9	39.6	29
CGGTCTA	540	0.0	39.518517	31
CTATCGT	45	2.3526809E-8	39.111107	19
TCCGATT	40	4.126814E-7	38.5	39
TACGTTG	40	4.126814E-7	38.5	1
CGTAAGG	395	0.0	38.430378	2
TTAGGGA	2985	0.0	37.956448	4
CGTTAGG	390	0.0	37.794872	2
AATGGGC	495	0.0	37.77778	4
TCTCACG	565	0.0	37.769913	23
CGTTACA	35	7.2932144E-6	37.714287	41
TAGGGAC	2625	0.0	37.714287	5
>>END_MODULE