FastQCFastQC Report
Thu 26 May 2016
SRR1545947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545947_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1709622
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171491.003087232148393No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27040.15816361745461863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG750.044.0000041
TTTAGCG502.7284841E-1144.01
CGCGAGT302.5284444E-644.015
GCGTTAA254.4437533E-544.09
ATCGTTC207.85756E-444.033
TACGCTA408.3164196E-944.025
TCTACGA254.4437533E-544.015
TTACGTG254.4437533E-544.026
ACGTAAG900.044.01
ACGTAAC254.4437533E-544.021
TTCGATC207.85756E-444.018
TCGGTAC454.802132E-1044.036
TATAGCG850.043.9999961
CGTTTTT131400.043.1126331
TTGCGAG750.041.066671
GCGTAAG1450.040.965521
TGGATCG650.040.61538734
GCGTTAG603.6379788E-1240.3333321
TACGATG501.3478711E-939.6000021
ATTAACG900.039.111111
Produced by FastQC (version 0.11.5)