##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545947_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1709622
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4808922674135	31.0	31.0	33.0	30.0	34.0
2	31.87544849095297	31.0	31.0	34.0	30.0	34.0
3	31.91726533701602	31.0	31.0	34.0	30.0	34.0
4	35.689300909791754	37.0	35.0	37.0	33.0	37.0
5	35.621327404537375	37.0	35.0	37.0	33.0	37.0
6	35.67698532190157	37.0	35.0	37.0	33.0	37.0
7	35.97928489455564	37.0	35.0	37.0	35.0	37.0
8	35.97097194584534	37.0	35.0	37.0	35.0	37.0
9	37.780060153648	39.0	38.0	39.0	35.0	39.0
10	37.2692513315809	39.0	37.0	39.0	34.0	39.0
11	36.90619739334192	39.0	37.0	39.0	33.0	39.0
12	36.163129042560286	38.0	35.0	39.0	32.0	39.0
13	35.83110477052822	38.0	35.0	39.0	31.0	39.0
14	36.806349590728246	39.0	35.0	41.0	31.0	41.0
15	37.038827296326325	39.0	35.0	41.0	32.0	41.0
16	37.22669397094796	39.0	35.0	41.0	32.0	41.0
17	37.14529586072243	39.0	35.0	41.0	32.0	41.0
18	37.02084554363479	38.0	35.0	40.0	32.0	41.0
19	36.863823114115284	38.0	35.0	40.0	32.0	41.0
20	36.616425151290755	38.0	35.0	40.0	32.0	41.0
21	36.48113852067884	38.0	35.0	40.0	31.0	41.0
22	36.392355737116155	37.0	35.0	40.0	31.0	41.0
23	36.34304717651036	37.0	35.0	40.0	31.0	41.0
24	36.377949043706735	37.0	35.0	40.0	31.0	41.0
25	36.33687797653516	37.0	35.0	40.0	31.0	41.0
26	36.10328306491142	37.0	35.0	40.0	31.0	41.0
27	36.07932221274644	37.0	35.0	40.0	31.0	41.0
28	36.0804084177672	37.0	35.0	40.0	31.0	41.0
29	36.02966328229281	37.0	35.0	40.0	31.0	41.0
30	35.90756202248216	37.0	35.0	40.0	30.0	41.0
31	35.653627527020596	37.0	35.0	40.0	30.0	41.0
32	35.36636110204478	37.0	34.0	40.0	29.0	41.0
33	35.06866605600536	37.0	34.0	40.0	27.0	41.0
34	34.77187881297737	37.0	34.0	40.0	24.0	41.0
35	34.49827388744413	36.0	34.0	40.0	23.0	41.0
36	34.285881908398466	36.0	34.0	40.0	23.0	41.0
37	34.20063089969596	36.0	33.0	40.0	23.0	41.0
38	34.12488901055321	36.0	33.0	40.0	23.0	41.0
39	34.028393995865756	36.0	33.0	40.0	22.0	41.0
40	33.88007992410018	36.0	33.0	40.0	21.0	41.0
41	33.845177471979184	36.0	33.0	40.0	21.0	41.0
42	33.799379044022594	36.0	33.0	40.0	20.0	41.0
43	33.70713058208189	35.0	33.0	40.0	20.0	41.0
44	33.580441758470585	35.0	33.0	40.0	19.0	41.0
45	33.474085499601664	35.0	33.0	40.0	20.0	41.0
46	33.452094088634794	35.0	33.0	40.0	20.0	41.0
47	33.39184860746995	35.0	33.0	40.0	20.0	41.0
48	33.29611165509101	35.0	33.0	40.0	18.0	41.0
49	33.25955386629325	35.0	33.0	40.0	18.0	41.0
50	33.073484665031216	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	14.0
15	50.0
16	135.0
17	343.0
18	855.0
19	1700.0
20	2962.0
21	4825.0
22	7529.0
23	11030.0
24	16163.0
25	25038.0
26	36143.0
27	43797.0
28	44037.0
29	42910.0
30	45810.0
31	52244.0
32	63912.0
33	82700.0
34	147864.0
35	250509.0
36	107676.0
37	136635.0
38	209739.0
39	374995.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.23063343826881	21.814939208784164	29.273839480306172	16.680587872640853
2	34.719721669468456	24.674869649548263	28.40703968479582	12.198368996187462
3	27.342067427770584	23.831993271027162	36.3979288989028	12.428010402299455
4	24.01238402407082	27.073411549453624	35.444911214291814	13.469293212183747
5	21.91244614306554	31.237080477438873	34.11906257640578	12.731410803089807
6	19.503258615062276	40.331663958465676	30.421286108859153	9.7437913176129
7	84.88993473411082	3.333660891120961	10.695814630368584	1.0805897443996393
8	86.51105332055859	2.2003109459283983	10.127794331144544	1.1608414023684768
9	83.15048589688246	3.7136864172314112	11.172762166139648	1.9630655197464701
10	47.98657247040573	27.722092953881035	15.69446345449462	8.596871121218609
11	41.1137081764273	19.404581831539367	27.507952050219288	11.973757941814037
12	37.715939546870594	20.11409539652625	28.799056165631935	13.37090889097122
13	21.174505241509525	35.09389794937127	29.585955257945905	14.145641551173298
14	15.758629685392444	36.8827144245921	31.897109419509107	15.461546470506345
15	14.183369189212586	22.883128551223603	47.91439277220345	15.019109487360364
16	16.90110445466893	18.757070276353485	46.30883318066801	18.03299208830958
17	17.749069677390676	18.36084233824787	32.10698037343928	31.78310761092218
18	21.758143028107966	21.87466001256418	37.30894899574292	19.058247963584932
19	26.96713074586078	23.040590259133307	29.5802814891245	20.411997505881416
20	29.033259983785893	22.293407548569217	29.7439433980143	18.9293890696306
21	21.987725941757887	26.290022004864234	32.37066439247974	19.35158766089814
22	22.026389459190394	21.547336194784577	30.895016559216014	25.531257786809014
23	19.088898013712974	26.80393677666759	30.79347364505136	23.313691564568074
24	19.388437912006278	22.448471065533784	40.81580606707214	17.3472849553878
25	17.63846043160418	24.03823769230859	37.78542859181737	20.537873284269857
26	16.94017741933597	31.444845702734288	31.110034849808905	20.504942028120837
27	17.57827168812755	30.592961485053422	33.18423604750056	18.64453077931847
28	15.431364360074918	26.49293235580731	40.446718631369976	17.6289846527478
29	16.290852597825715	22.89038161652108	39.907710593335835	20.911055192317367
30	19.312865651003555	27.469756472483393	35.061902572615466	18.155475303897585
31	27.84165154636522	24.39825879638891	28.933179381173147	18.826910276072724
32	27.419511447559753	25.0326095476076	29.943285708770713	17.60459329606194
33	26.128933764305796	26.314179391701792	28.210505012219073	19.34638183177334
34	19.640949870790152	26.297859994782474	30.979128719681892	23.082061414745482
35	19.57034946906392	25.419186229470608	32.72208710463483	22.288377196830645
36	28.791861592796536	24.056662817862662	29.09420912926951	18.057266460071293
37	19.65990142850291	30.25388068239646	30.426140983211493	19.660076905889138
38	20.331628862988428	30.95526379515472	27.225140996079833	21.48796634577702
39	20.68626866055771	29.40638339937132	28.982839481476024	20.92450845859494
40	23.425880106830633	25.54827909327325	28.06977214846323	22.95606865143289
41	19.255133590934136	23.305385634953225	29.27582822401677	28.16365255009587
42	22.133840112024764	24.483599298558396	26.76357697783487	26.618983611581974
43	21.9470151881527	26.03411748327993	27.037087730504172	24.9817795980632
44	20.05770866308459	28.469509634293427	29.53032892651124	21.942452776110745
45	18.537489573718634	34.304542173650084	25.63958582657453	21.518382426056753
46	22.61529156737571	30.15391706470787	27.32399325698897	19.906798110927447
47	21.575412576581257	26.607109641780465	28.422774157094377	23.394703624543904
48	22.371085538206692	24.088892164466767	31.987948213113775	21.552074084212766
49	21.71830966143393	23.432840709817725	33.023030822017965	21.825818806730375
50	19.853043538279223	30.139995858733688	29.612276865880293	20.3946837371068
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	523.0
1	1381.0
2	2239.0
3	16712.5
4	31186.0
5	23017.0
6	14848.0
7	15029.0
8	15210.0
9	15609.5
10	16009.0
11	15773.5
12	15538.0
13	14436.0
14	13334.0
15	12284.5
16	11235.0
17	10550.5
18	9866.0
19	9291.5
20	8717.0
21	8296.5
22	7876.0
23	8214.5
24	8553.0
25	8786.0
26	9019.0
27	11316.5
28	13614.0
29	16657.0
30	19700.0
31	24968.0
32	30236.0
33	34563.5
34	38891.0
35	41986.5
36	45082.0
37	49609.5
38	54137.0
39	67948.0
40	81759.0
41	104424.0
42	127089.0
43	147174.5
44	167260.0
45	167198.0
46	167136.0
47	156779.0
48	146422.0
49	137384.0
50	128346.0
51	119205.5
52	110065.0
53	100988.5
54	91912.0
55	83912.0
56	75912.0
57	67901.0
58	59890.0
59	55831.0
60	51772.0
61	46634.0
62	41496.0
63	36147.0
64	30798.0
65	26779.5
66	22761.0
67	19590.5
68	16420.0
69	13449.0
70	10478.0
71	7835.5
72	5193.0
73	4722.5
74	4252.0
75	3322.0
76	2392.0
77	1758.5
78	1125.0
79	931.0
80	737.0
81	540.5
82	344.0
83	249.5
84	155.0
85	104.0
86	53.0
87	42.0
88	31.0
89	18.0
90	5.0
91	5.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1709622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.060945139129306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.73108454398768	15.994809129202173
2	7.1744893600573025	2.8785407490675285
3	2.506217508956032	1.508312758616768
4	1.3311748246461381	1.0681850051126498
5	0.874078149739143	0.8767416904614297
6	0.6497762932277379	0.7821075942689066
7	0.5130980583257049	0.7205262399346013
8	0.40996348653919756	0.6579404010007214
9	0.35198281217429184	0.635499709645043
>10	3.9391134742507923	18.764715334345937
>50	1.3966040792465093	20.157574830964766
>100	1.1188757288846585	34.38337357138082
>500	0.0026562599735938646	0.3158147940221171
>1k	5.902799941319699E-4	0.24050237969963248
>5k	0.0	0.0
>10k+	2.9513999706598493E-4	1.0153558122769566
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17149	1.003087232148393	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2704	0.15816361745461863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025678190851544963	0.0
2	0.0	0.0	0.0	0.11458673320769153	0.0
3	0.0	0.0	0.0	0.16892623047667846	0.0
4	0.0	0.0	0.0	0.2579517577569779	0.0
5	0.0	0.0	0.0	0.4719756764945701	0.0
6	0.0	0.0	0.0	0.6319525602735575	0.0
7	0.0	0.0	0.0	0.7453109517776444	0.0
8	0.0	0.0	0.0	1.0113931617632437	0.0
9	0.0	0.0	0.0	1.1152757744109516	0.0
10	0.0	0.0	0.0	1.392588537115222	0.0
11	0.0	0.0	0.0	1.5892986870781962	0.0
12	0.0	0.0	0.0	1.7924430078695759	0.0
13	0.0	0.0	0.0	1.8643302437614864	0.0
14	0.0	0.0	0.0	1.8901254195371842	0.0
15	0.0	0.0	0.0	1.9391421027572178	0.0
16	0.0	0.0	0.0	2.0445455194189126	0.0
17	0.0	0.0	0.0	2.1720005942834146	0.0
18	0.0	0.0	0.0	2.372805216591738	0.0
19	0.0	0.0	0.0	2.4634685328101766	0.0
20	0.0	0.0	0.0	2.5616188841743965	0.0
21	0.0	0.0	0.0	2.6894834062734336	0.0
22	0.0	0.0	0.0	2.8226707424214244	0.0
23	0.0	0.0	0.0	2.9728793850336506	0.0
24	0.0	0.0	0.0	3.088577475020794	0.0
25	0.0	0.0	0.0	3.1809955651015254	0.0
26	0.0	0.0	0.0	3.273179685333951	0.0
27	0.0	0.0	0.0	3.372382901015546	0.0
28	0.0	0.0	0.0	3.473808830256045	0.0
29	0.0	0.0	0.0	3.588161593615431	0.0
30	0.0	0.0	0.0	3.7361475226687535	0.0
31	0.0	0.0	0.0	3.8698027985133554	0.0
32	0.0	0.0	0.0	3.9870801849765622	0.0
33	0.0	0.0	0.0	4.106112345302061	0.0
34	0.0	0.0	0.0	4.236901490504919	0.0
35	5.849246207641221E-5	0.0	0.0	4.399393550153191	0.0
36	5.849246207641221E-5	0.0	0.0	4.531995961680418	0.0
37	5.849246207641221E-5	0.0	0.0	4.668575860628841	0.0
38	5.849246207641221E-5	0.0	0.0	4.82200158865527	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	75	0.0	44.000004	1
TTTAGCG	50	2.7284841E-11	44.0	1
CGCGAGT	30	2.5284444E-6	44.0	15
GCGTTAA	25	4.4437533E-5	44.0	9
ATCGTTC	20	7.85756E-4	44.0	33
TACGCTA	40	8.3164196E-9	44.0	25
TCTACGA	25	4.4437533E-5	44.0	15
TTACGTG	25	4.4437533E-5	44.0	26
ACGTAAG	90	0.0	44.0	1
ACGTAAC	25	4.4437533E-5	44.0	21
TTCGATC	20	7.85756E-4	44.0	18
TCGGTAC	45	4.802132E-10	44.0	36
TATAGCG	85	0.0	43.999996	1
CGTTTTT	13140	0.0	43.112633	1
TTGCGAG	75	0.0	41.06667	1
GCGTAAG	145	0.0	40.96552	1
TGGATCG	65	0.0	40.615387	34
GCGTTAG	60	3.6379788E-12	40.333332	1
TACGATG	50	1.3478711E-9	39.600002	1
ATTAACG	90	0.0	39.11111	1
>>END_MODULE