FastQCFastQC Report
Thu 26 May 2016
SRR1545946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545946_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1354712
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131970.9741553924376546No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25180.18586976420080428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA254.443264E-544.010
AAGTACG351.446333E-744.01
GCGAATC302.5280533E-644.034
TAGACGT254.443264E-544.024
CTATACG600.044.01
TGTATCG207.856981E-444.011
TAAGTCG207.856981E-444.01
CCTCGCG254.443264E-544.025
CCGTCCA207.856981E-444.033
CTCGGTA254.443264E-544.021
AAGTTCG207.856981E-444.022
CGTAGGT207.856981E-444.036
GAACGTA254.443264E-544.09
ATAACGG800.044.02
ATACGAG351.446333E-744.01
CCACGCG207.856981E-444.028
TATCGTG207.856981E-444.01
CCGTTGT207.856981E-444.013
TACACCG207.856981E-444.035
TACGTTG454.802132E-1044.01
Produced by FastQC (version 0.11.5)