##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545946_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1354712
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46073999492143	31.0	31.0	33.0	30.0	34.0
2	31.847607461955015	31.0	31.0	34.0	30.0	34.0
3	31.899078918618866	31.0	31.0	34.0	30.0	34.0
4	35.66402748333225	37.0	35.0	37.0	33.0	37.0
5	35.593106874376254	37.0	35.0	37.0	33.0	37.0
6	35.640519165697214	37.0	35.0	37.0	33.0	37.0
7	35.94771656263472	37.0	35.0	37.0	35.0	37.0
8	35.94558991136123	37.0	35.0	37.0	35.0	37.0
9	37.76166963900814	39.0	38.0	39.0	35.0	39.0
10	37.258140475614006	39.0	37.0	39.0	34.0	39.0
11	36.85816690189502	39.0	35.0	39.0	33.0	39.0
12	35.67358449618812	37.0	35.0	39.0	32.0	39.0
13	35.15177764720472	37.0	35.0	39.0	30.0	39.0
14	35.91504393553759	38.0	35.0	41.0	30.0	41.0
15	36.30505302972145	38.0	35.0	41.0	31.0	41.0
16	36.58474199682294	38.0	35.0	41.0	32.0	41.0
17	36.56184266471398	37.0	35.0	41.0	32.0	41.0
18	36.47586719538913	37.0	35.0	40.0	32.0	41.0
19	36.30532836499566	37.0	35.0	40.0	31.0	41.0
20	36.035302706405496	36.0	35.0	40.0	31.0	41.0
21	35.83094340346878	35.0	35.0	40.0	31.0	41.0
22	35.723752354744036	35.0	35.0	40.0	31.0	41.0
23	35.71833939612257	35.0	34.0	40.0	31.0	41.0
24	35.71568052840751	35.0	35.0	40.0	31.0	41.0
25	35.657792947873794	35.0	35.0	40.0	31.0	41.0
26	35.40005920077478	35.0	34.0	40.0	30.0	41.0
27	35.36237222376417	35.0	34.0	40.0	30.0	41.0
28	35.36303214262515	35.0	34.0	40.0	30.0	41.0
29	35.35024639923467	35.0	34.0	40.0	30.0	41.0
30	35.244958338008374	36.0	34.0	40.0	30.0	41.0
31	34.93622925020226	35.0	34.0	40.0	29.0	41.0
32	34.62459179515646	35.0	34.0	40.0	27.0	41.0
33	34.37356500865128	35.0	34.0	40.0	25.0	41.0
34	34.11763607320228	35.0	34.0	40.0	23.0	41.0
35	33.87140883080684	35.0	33.0	40.0	23.0	41.0
36	33.63935581880134	35.0	33.0	40.0	22.0	41.0
37	33.509272819610366	35.0	33.0	40.0	22.0	41.0
38	33.4297444770549	35.0	33.0	40.0	22.0	41.0
39	33.36043454254484	35.0	33.0	39.0	21.0	41.0
40	33.18431592840397	35.0	33.0	39.0	20.0	41.0
41	33.15535257678385	35.0	33.0	39.0	20.0	41.0
42	33.11381976390554	35.0	33.0	39.0	20.0	41.0
43	32.99925740674033	35.0	33.0	39.0	19.0	41.0
44	32.86681449636528	35.0	32.0	39.0	18.0	41.0
45	32.743352092548086	35.0	32.0	39.0	18.0	41.0
46	32.74546693319318	35.0	32.0	39.0	18.0	41.0
47	32.69139861461329	35.0	32.0	38.0	18.0	41.0
48	32.609232811106715	35.0	32.0	38.0	18.0	41.0
49	32.60438454815488	35.0	32.0	38.0	18.0	40.0
50	32.38771192696307	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	5.0
14	16.0
15	57.0
16	142.0
17	354.0
18	797.0
19	1732.0
20	2947.0
21	4630.0
22	6880.0
23	9948.0
24	14635.0
25	21751.0
26	30488.0
27	36981.0
28	37837.0
29	37183.0
30	40618.0
31	46424.0
32	56985.0
33	75156.0
34	143760.0
35	254907.0
36	78925.0
37	94465.0
38	138037.0
39	219049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.828536249771165	21.874317198046523	29.206724381270703	17.090422170911605
2	33.35173822923249	23.63875126226091	30.018336000566908	12.991174507939697
3	27.74515911869091	23.377884007818636	35.90135763173279	12.975599241757656
4	24.025623158280133	26.70715251654964	34.98581248265314	14.28141184251708
5	21.240676985218997	31.41228541564554	33.72237051122305	13.624667087912412
6	19.60158321473494	39.78727581950998	30.79008674906548	9.8210542166896
7	85.19714891430799	3.195660775131541	10.273475100242708	1.333715210317765
8	87.05909447912175	1.991862477043091	9.598792953779107	1.3502500900560415
9	84.58705614182203	2.8305647251962043	10.816321107364518	1.766058025617253
10	58.731302298938814	17.27555377083838	14.99971949757587	8.99342443264694
11	53.53329711407295	16.348936157648268	19.301814703051274	10.815952025227501
12	45.774526246168925	19.005367930600748	23.746227980559706	11.47387784267062
13	21.70188202363307	41.39809789829868	25.07307826312899	11.826941814939264
14	13.911739174082758	43.42561370977743	30.844637088916315	11.818010027223497
15	12.71797991012112	19.739546117551185	54.49165579104637	13.050818181281334
16	14.728813209006786	14.060553091727247	52.3100112791501	18.900622420115862
17	16.458332103059544	14.059372028888797	31.216155168035716	38.266140700015946
18	23.15975646484271	19.57028504951606	37.22178588511802	20.048172600523213
19	29.584590673146767	22.044537879637886	27.828940763793337	20.54193068342201
20	32.303840225819215	21.213438723507284	25.93842824157459	20.544292809098906
21	22.087499040386444	25.44341527940994	31.229146859258645	21.239938820944968
22	22.411479340258296	21.530996994195075	29.053481477982036	27.004042187564593
23	18.989349765854293	27.10871388162207	27.65037882590543	26.251557526618203
24	20.260690095016507	21.67973709541216	41.15841595852107	16.90115685105026
25	17.618357259698	21.057464612404704	40.11443022576016	21.20974790213713
26	15.81029768688843	32.129264375011076	30.26835224018094	21.792085697919557
27	16.775226025900707	30.95041602938484	33.48527214640455	18.789085798309898
28	13.960162750459137	25.154940681118937	43.803627634508295	17.08126893391363
29	14.701132048730653	22.862571528118153	42.21074294757853	20.22555347557267
30	19.4563124856058	26.45588139766976	35.347808242637555	18.739997874086892
31	31.422324449772347	23.40460555453853	26.667808360743834	18.50526163494529
32	31.23726666627298	24.42821795333621	28.034076615546333	16.300438764844483
33	28.5808348933205	25.321544357767557	26.839874453020272	19.25774629589167
34	18.460233614229445	26.526818984404066	30.623630705271676	24.389316696094816
35	19.449964272849137	24.610913611158683	32.1738494971625	23.765272618829687
36	31.994770844282773	21.43156626648321	27.188214173935126	19.385448715298896
37	20.141845646897643	30.141683250757357	29.899196286738437	19.817274815606563
38	20.781760256054422	32.122030365125575	23.612841696242448	23.48336768257755
39	20.169010092181956	29.65095164138208	27.158613786546514	23.02142447988945
40	23.843370399022078	24.087628957298673	25.557166394037996	26.511834249641254
41	18.102150124897396	21.367050708932968	28.50140841743485	32.029390748734784
42	23.299121879779612	23.78106933429393	24.45434896863688	28.465459817289577
43	23.67211628744707	24.52631998535482	25.909566018460012	25.891997708738096
44	20.70004547091928	29.283419649342445	28.28734077796609	21.729194101772183
45	18.198923461222755	36.09925947360029	23.094576559445844	22.607240505731106
46	23.701273776271268	30.07266489113553	26.47728816161664	19.74877317097656
47	21.836227921506563	27.107828084493235	27.404201040516362	23.65174295348384
48	23.820487306527145	23.229512988738566	30.774659115738253	22.175340588996036
49	21.48146617140765	21.813713911148643	32.605897046752375	24.098922870691336
50	19.633545727800445	30.99780617577758	28.02233980358925	21.34630829283272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	337.0
1	925.0
2	1513.0
3	11689.0
4	21865.0
5	15968.5
6	10072.0
7	10493.0
8	10914.0
9	11568.5
10	12223.0
11	12130.0
12	12037.0
13	11259.5
14	10482.0
15	9389.0
16	8296.0
17	7383.0
18	6470.0
19	6213.0
20	5956.0
21	5521.5
22	5087.0
23	4678.0
24	4269.0
25	4454.0
26	4639.0
27	5954.0
28	7269.0
29	7957.5
30	8646.0
31	9960.5
32	11275.0
33	12928.0
34	14581.0
35	16917.5
36	19254.0
37	22588.5
38	25923.0
39	38142.5
40	50362.0
41	79044.0
42	107726.0
43	127871.5
44	148017.0
45	147917.0
46	147817.0
47	133887.0
48	119957.0
49	117112.0
50	114267.0
51	105838.5
52	97410.0
53	87076.0
54	76742.0
55	69590.5
56	62439.0
57	58953.0
58	55467.0
59	50397.5
60	45328.0
61	40239.0
62	35150.0
63	33018.0
64	30886.0
65	26017.0
66	21148.0
67	17881.5
68	14615.0
69	12619.0
70	10623.0
71	8600.5
72	6578.0
73	5355.0
74	4132.0
75	3269.5
76	2407.0
77	1929.0
78	1451.0
79	987.5
80	524.0
81	433.0
82	342.0
83	226.5
84	111.0
85	80.5
86	50.0
87	42.0
88	34.0
89	25.0
90	16.0
91	10.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1354712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.367341636607925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.40671139182368	17.31382358667231
2	8.134977379741478	3.639156365175104
3	3.39885785927908	2.280702447382952
4	1.9922731958657558	1.7824742082154423
5	1.3253018547140571	1.482173967800972
6	0.9432319372714425	1.2658554590105935
7	0.7310011059252194	1.144538603107732
8	0.5384460872314667	0.9634886068800805
9	0.4067422815650439	0.818796921283684
>10	3.0067279172992043	14.79125181257662
>50	1.0877647115116886	17.708364690291855
>100	1.0232871572361057	35.01126990370627
>500	0.004008960459298883	0.623802826583501
>1k	3.3408003827490695E-4	0.18815710557465926
>5k	0.0	0.0
>10k+	3.3408003827490695E-4	0.9861434957381963
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13197	0.9741553924376546	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2518	0.18586976420080428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03993468722503381	0.0
2	0.0	0.0	0.0	0.1500687969103396	0.0
3	0.0	0.0	0.0	0.21015536881639788	0.0
4	0.0	0.0	0.0	0.3172630049781799	0.0
5	0.0	0.0	0.0	0.5449128670890935	0.0
6	0.0	0.0	0.0	0.7517464966723555	0.0
7	0.0	0.0	0.0	0.8996746171880075	0.0
8	0.0	0.0	0.0	1.293337624528313	0.0
9	0.0	0.0	0.0	1.4429635228742346	0.0
10	0.0	0.0	0.0	1.7044951251631344	0.0
11	0.0	0.0	0.0	1.9159053732453835	0.0
12	0.0	0.0	0.0	2.120229244296943	0.0
13	0.0	0.0	0.0	2.2027560101335193	0.0
14	0.0	0.0	0.0	2.2307324361192635	0.0
15	0.0	0.0	0.0	2.2760557225447178	0.0
16	0.0	0.0	0.0	2.3852302186737844	0.0
17	0.0	0.0	0.0	2.51197302452477	0.0
18	0.0	0.0	0.0	2.7245643354454674	0.0
19	0.0	0.0	0.0	2.7963877193086057	0.0
20	0.0	0.0	0.0	2.8830482050797515	0.0
21	0.0	0.0	0.0	3.000859223214971	0.0
22	0.0	0.0	0.0	3.115200869262249	0.0
23	0.0	0.0	0.0	3.2591429026981382	0.0
24	0.0	0.0	0.0	3.35525189117687	0.0
25	0.0	0.0	0.0	3.4304708307005476	0.0
26	0.0	0.0	0.0	3.520674504987038	0.0
27	0.0	0.0	0.0	3.6070397250485713	0.0
28	0.0	0.0	0.0	3.6993102593023464	0.0
29	0.0	0.0	0.0	3.8213288137995383	0.0
30	0.0	0.0	0.0	3.9747193499430136	0.0
31	0.0	0.0	0.0	4.101535972221402	0.0
32	0.0	0.0	0.0	4.2042884391664055	0.0
33	0.0	0.0	0.0	4.317744288084848	0.0
34	0.0	0.0	0.0	4.443306031097385	0.0
35	0.0	0.0	0.0	4.608211929915731	0.0
36	0.0	0.0	0.0	4.73259261008982	0.0
37	0.0	0.0	0.0	4.8490011161043824	0.0
38	0.0	0.0	0.0	4.997150685902244	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	25	4.443264E-5	44.0	10
AAGTACG	35	1.446333E-7	44.0	1
GCGAATC	30	2.5280533E-6	44.0	34
TAGACGT	25	4.443264E-5	44.0	24
CTATACG	60	0.0	44.0	1
TGTATCG	20	7.856981E-4	44.0	11
TAAGTCG	20	7.856981E-4	44.0	1
CCTCGCG	25	4.443264E-5	44.0	25
CCGTCCA	20	7.856981E-4	44.0	33
CTCGGTA	25	4.443264E-5	44.0	21
AAGTTCG	20	7.856981E-4	44.0	22
CGTAGGT	20	7.856981E-4	44.0	36
GAACGTA	25	4.443264E-5	44.0	9
ATAACGG	80	0.0	44.0	2
ATACGAG	35	1.446333E-7	44.0	1
CCACGCG	20	7.856981E-4	44.0	28
TATCGTG	20	7.856981E-4	44.0	1
CCGTTGT	20	7.856981E-4	44.0	13
TACACCG	20	7.856981E-4	44.0	35
TACGTTG	45	4.802132E-10	44.0	1
>>END_MODULE