FastQCFastQC Report
Thu 26 May 2016
SRR1545945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545945_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1197282
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137891.1516919155219907No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26260.21933011604617791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT207.856613E-444.00000413
ATTACGC207.856613E-444.00000411
TCGATAG207.856613E-444.0000041
ACCGGTA351.4461511E-744.025
TAACGCG454.802132E-1044.01
GCGTAAG454.802132E-1044.01
CGTTTTT110700.043.3640441
CGTAAGG2050.042.926832
GTTACGG2250.041.0666662
TAATACG700.040.8571431
GCGTATG650.040.6153871
CGGGTCA603.6379788E-1240.3333326
TTACGGG4700.040.255323
TACGGGA3500.040.228574
AATACGG1700.040.117652
TTTAGCG557.8216544E-1140.0000041
TGCGAAG557.8216544E-1140.0000041
CGACGGT1600.039.87500428
CGGTCTA1600.039.87500431
TTGCTCG452.3510438E-839.1111110
Produced by FastQC (version 0.11.5)