##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545945_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1197282
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55073324413129	31.0	31.0	33.0	30.0	34.0
2	31.954702400938125	31.0	31.0	34.0	30.0	34.0
3	31.9658459744655	33.0	31.0	34.0	30.0	34.0
4	35.74573408770866	37.0	35.0	37.0	35.0	37.0
5	35.69513197392093	37.0	35.0	37.0	33.0	37.0
6	35.75759762528794	37.0	35.0	37.0	33.0	37.0
7	36.01062072260336	37.0	35.0	37.0	35.0	37.0
8	36.01377035652419	37.0	35.0	37.0	35.0	37.0
9	37.832919061674694	39.0	38.0	39.0	35.0	39.0
10	37.335871582467625	39.0	37.0	39.0	34.0	39.0
11	37.017928107162724	39.0	37.0	39.0	33.0	39.0
12	36.238296408030855	38.0	35.0	39.0	32.0	39.0
13	35.88018528634023	38.0	35.0	39.0	32.0	39.0
14	36.870827424115625	39.0	35.0	41.0	31.0	41.0
15	37.103780897065185	39.0	35.0	41.0	32.0	41.0
16	37.284392482305755	39.0	35.0	41.0	33.0	41.0
17	37.216806900964016	39.0	35.0	41.0	33.0	41.0
18	37.09386176356113	38.0	35.0	40.0	32.0	41.0
19	36.91263378218331	37.0	35.0	40.0	32.0	41.0
20	36.650881747157314	37.0	35.0	40.0	32.0	41.0
21	36.51006446267463	37.0	35.0	40.0	32.0	41.0
22	36.4006867220922	37.0	35.0	40.0	31.0	41.0
23	36.370393942279264	37.0	35.0	40.0	32.0	41.0
24	36.388876638920486	37.0	35.0	40.0	32.0	41.0
25	36.33630506430398	37.0	35.0	40.0	32.0	41.0
26	36.12609560654883	37.0	35.0	40.0	31.0	41.0
27	36.10627738494357	37.0	35.0	40.0	31.0	41.0
28	36.09127340091975	37.0	35.0	40.0	31.0	41.0
29	36.021580546604724	36.0	35.0	40.0	31.0	41.0
30	35.9131374229296	36.0	35.0	40.0	31.0	41.0
31	35.6371197428843	36.0	35.0	40.0	30.0	41.0
32	35.32755190506497	36.0	34.0	40.0	29.0	41.0
33	34.99232177548815	36.0	34.0	40.0	27.0	41.0
34	34.66166784433408	36.0	34.0	40.0	24.0	41.0
35	34.375652519623614	36.0	34.0	40.0	23.0	41.0
36	34.15466615216799	36.0	34.0	40.0	21.0	41.0
37	34.04750092292375	36.0	33.0	40.0	21.0	41.0
38	33.943542958133506	36.0	33.0	40.0	21.0	41.0
39	33.84997936993958	36.0	33.0	40.0	20.0	41.0
40	33.6624613081964	36.0	33.0	40.0	18.0	41.0
41	33.63445955088275	36.0	33.0	40.0	18.0	41.0
42	33.595992422837725	36.0	33.0	40.0	18.0	41.0
43	33.48639752372457	35.0	33.0	40.0	18.0	41.0
44	33.34594606784366	35.0	33.0	40.0	18.0	41.0
45	33.25632474220777	35.0	33.0	40.0	18.0	41.0
46	33.214004720692365	35.0	33.0	40.0	18.0	41.0
47	33.15193747170675	35.0	33.0	40.0	18.0	41.0
48	33.069611837478554	35.0	33.0	40.0	18.0	41.0
49	33.02985261617564	35.0	33.0	40.0	18.0	41.0
50	32.86363613584769	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	11.0
15	25.0
16	83.0
17	244.0
18	533.0
19	1145.0
20	2032.0
21	3249.0
22	5000.0
23	7581.0
24	11691.0
25	18059.0
26	26810.0
27	32403.0
28	31841.0
29	30245.0
30	31658.0
31	35823.0
32	43272.0
33	56889.0
34	104658.0
35	181451.0
36	73588.0
37	94461.0
38	146571.0
39	257949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.38548980106608	22.44558925967316	28.558935990017385	18.60998494924337
2	35.30479870239426	24.055151585006705	28.77166782762958	11.868381884969455
3	27.05586486725767	23.496385980913438	36.79108180027763	12.656667351551262
4	23.888440651408775	26.048416329653328	36.184541319421825	13.878601699516071
5	20.956884008946933	30.843777823436753	34.62809931160746	13.571238856008861
6	19.152964798602166	39.442587460598254	31.186136599397635	10.218311141401943
7	84.2138276529673	3.2044246885863146	11.453776136282013	1.1279715221643691
8	85.95351805172048	1.9065683773747537	11.090954344924588	1.0489592259801785
9	82.42201920683682	3.6029105924919946	12.107924448876705	1.8671457517944812
10	47.91168663689924	24.506590761407924	17.630182363052313	9.95154023864052
11	42.10194423703021	20.056594854011	25.600819188795953	12.240641720162836
12	38.775576681182876	19.419401611316296	28.706186178360653	13.098835529140167
13	21.20118735602807	35.65751426982114	29.038939865461938	14.102358508688848
14	15.475886215611695	37.833526270335646	31.51889028649892	15.171697227553743
15	14.70689444926091	20.883801811102146	49.1012977727887	15.308005966848246
16	16.57938564181204	17.546409283694235	47.578264769703374	18.29594030479035
17	16.834296347894647	17.770333137890656	32.466787273173736	32.92858324104096
18	22.03666304178965	21.602680070359366	36.67824288680528	19.6824140010457
19	26.59615696218602	23.05839392891566	29.956434657833324	20.389014451064995
20	28.561692232907536	21.687455419859315	29.56446350984981	20.18638883738334
21	21.637676002813038	24.836170593059947	33.11726059524824	20.408892808878775
22	22.422704091433765	22.3611480002205	31.174192880207002	24.041955028138734
23	18.598458842611848	27.175135014140363	30.10710926916132	24.119296874086473
24	19.961629758068693	22.623241642319854	40.374865737562246	17.040262862049207
25	17.28757301955596	23.369431762943066	38.14356183422118	21.199433383279796
26	17.229107261280134	30.6419039123615	31.37581622374679	20.753172602611585
27	17.30962296267713	29.70762109511377	33.76564585452717	19.21711008768193
28	15.9206435910671	25.36269650758969	41.343643352192714	17.373016549150492
29	16.07382387774977	22.687470453911445	40.903897327446664	20.33480834089212
30	18.87132688873632	26.726201513093823	36.09567336684257	18.30679823132729
31	27.39446513018654	23.90748378410433	30.106775179114027	18.591275906595104
32	27.091445457294107	24.899814747068778	30.228885091398684	17.779854704238435
33	25.741304053681592	26.070299227750855	28.94656396738613	19.241832751181427
34	19.524472931189145	25.599065216047684	31.979349894176977	22.897111958586198
35	19.71599005079839	26.114148546457727	32.62798572099138	21.541875681752504
36	28.381534174906164	23.711372926344836	29.262696674634714	18.644396224114285
37	20.306577731896077	29.654584300106407	30.772783688387534	19.266054279609985
38	20.50335676975015	30.03010151326087	26.608434771423944	22.858106945565037
39	20.57009125669642	28.54106217248735	29.388398054927745	21.500448515888486
40	23.447441788985383	26.23617493623056	27.37600665507374	22.94037661971031
41	18.533979463484794	23.77794036826746	30.363857470504023	27.32422269774372
42	22.71937605342768	25.00872810248546	26.370729702776792	25.901166141310068
43	22.905547732280283	25.544357970803873	26.919973740522284	24.630120556393564
44	20.26799033143403	29.417547411553834	29.159462850021967	21.154999406990164
45	19.06392980099926	34.09380580347821	24.87910116413677	21.963163231385753
46	22.640447279755314	29.73267784866055	27.935022826702482	19.691852044881657
47	21.314276837035887	27.259910363640312	29.128392475623954	22.297420323699846
48	23.93279110518658	23.84091634218171	31.057344886167172	21.16894766646454
49	21.333069402195974	23.51476093351441	32.06345706358235	23.088712600707268
50	19.584692662213246	30.136091580763765	29.265035304965746	21.014180452057243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	1017.5
2	1655.0
3	12893.0
4	24131.0
5	17685.0
6	11239.0
7	11408.0
8	11577.0
9	12003.0
10	12429.0
11	12207.5
12	11986.0
13	11328.5
14	10671.0
15	9737.5
16	8804.0
17	8026.5
18	7249.0
19	6802.0
20	6355.0
21	6064.5
22	5774.0
23	5816.5
24	5859.0
25	6673.5
26	7488.0
27	8138.0
28	8788.0
29	9898.0
30	11008.0
31	13951.0
32	16894.0
33	20531.0
34	24168.0
35	26414.5
36	28661.0
37	32553.0
38	36445.0
39	46717.5
40	56990.0
41	73782.5
42	90575.0
43	100740.0
44	110905.0
45	108386.5
46	105868.0
47	104582.0
48	103296.0
49	99195.0
50	95094.0
51	87540.5
52	79987.0
53	71461.5
54	62936.0
55	58188.5
56	53441.0
57	50368.0
58	47295.0
59	42314.5
60	37334.0
61	33545.0
62	29756.0
63	26660.5
64	23565.0
65	19774.5
66	15984.0
67	13372.5
68	10761.0
69	9168.5
70	7576.0
71	7080.5
72	6585.0
73	5308.5
74	4032.0
75	2949.5
76	1867.0
77	1466.5
78	1066.0
79	735.0
80	404.0
81	295.5
82	187.0
83	142.0
84	97.0
85	75.0
86	53.0
87	50.0
88	47.0
89	27.5
90	8.0
91	7.5
92	7.0
93	5.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1197282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.939123717997113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93169340816036	17.31692186952647
2	6.771912395516198	2.9713964770533616
3	2.562524769877273	1.686586438703087
4	1.5246765256465082	1.3380026770433897
5	1.0328308444866758	1.132970183847831
6	0.7890159889616061	1.038619163838391
7	0.6133798661089566	0.9419911738085034
8	0.5003538842262603	0.8781860615056262
9	0.3980566907629382	0.7859713486882158
>10	4.557692688425538	23.576145641797275
>50	1.5519202001911891	24.441023785243722
>100	0.7651721715020937	22.504662510983675
>500	0.0	0.0
>1k	3.85283067221598E-4	0.2219698157821632
>5k	0.0	0.0
>10k+	3.85283067221598E-4	1.1655528521783123
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13789	1.1516919155219907	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2626	0.21933011604617791	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01603632226994142	0.0
2	0.0	0.0	0.0	0.05879984832311853	0.0
3	0.0	0.0	0.0	0.0875315923900969	0.0
4	0.0	0.0	0.0	0.13071273100238706	0.0
5	0.0	0.0	0.0	0.22551078192105117	0.0
6	0.0	0.0	0.0	0.32164519302887706	0.0
7	0.0	0.0	0.0	0.39322398566085515	0.0
8	0.0	0.0	0.0	0.6057887782494016	0.0
9	0.0	0.0	0.0	0.7155373587843131	0.0
10	0.0	0.0	0.0	0.9270998812309882	0.0
11	0.0	0.0	0.0	1.1276374321170786	0.0
12	0.0	0.0	0.0	1.3112199131031788	0.0
13	0.0	0.0	0.0	1.405349783927262	0.0
14	0.0	0.0	0.0	1.4499508052405365	0.0
15	0.0	0.0	0.0	1.4903757009626806	0.0
16	0.0	0.0	0.0	1.5825845540148435	0.0
17	0.0	0.0	0.0	1.6894933691477865	0.0
18	0.0	0.0	0.0	1.8517776096191207	0.0
19	0.0	0.0	0.0	1.9215189069910013	0.0
20	0.0	0.0	0.0	2.00846584179834	0.0
21	0.0	0.0	0.0	2.110112738686458	0.0
22	0.0	0.0	0.0	2.2121772481336897	0.0
23	0.0	0.0	0.0	2.330111034827217	0.0
24	0.0	0.0	0.0	2.414802861815345	0.0
25	0.0	0.0	0.0	2.4843771141635806	0.0
26	0.0	0.0	0.0	2.559296807268463	0.0
27	0.0	0.0	0.0	2.6514221378088036	0.0
28	0.0	0.0	0.0	2.729849776410236	0.0
29	0.0	0.0	0.0	2.825065439888013	0.0
30	0.0	0.0	0.0	2.9434168391406534	0.0
31	0.0	0.0	0.0	3.05299837465192	0.0
32	0.0	0.0	0.0	3.148882218224278	0.0
33	0.0	0.0	0.0	3.2524501328843165	0.0
34	0.0	0.0	0.0	3.3606117856945983	0.0
35	0.0	0.0	0.0	3.492911444421615	0.0
36	0.0	0.0	0.0	3.5966464041052983	0.0
37	0.0	0.0	0.0	3.7074807772939042	0.0
38	0.0	0.0	0.0	3.8369406706189517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	20	7.856613E-4	44.000004	13
ATTACGC	20	7.856613E-4	44.000004	11
TCGATAG	20	7.856613E-4	44.000004	1
ACCGGTA	35	1.4461511E-7	44.0	25
TAACGCG	45	4.802132E-10	44.0	1
GCGTAAG	45	4.802132E-10	44.0	1
CGTTTTT	11070	0.0	43.364044	1
CGTAAGG	205	0.0	42.92683	2
GTTACGG	225	0.0	41.066666	2
TAATACG	70	0.0	40.857143	1
GCGTATG	65	0.0	40.615387	1
CGGGTCA	60	3.6379788E-12	40.333332	6
TTACGGG	470	0.0	40.25532	3
TACGGGA	350	0.0	40.22857	4
AATACGG	170	0.0	40.11765	2
TTTAGCG	55	7.8216544E-11	40.000004	1
TGCGAAG	55	7.8216544E-11	40.000004	1
CGACGGT	160	0.0	39.875004	28
CGGTCTA	160	0.0	39.875004	31
TTGCTCG	45	2.3510438E-8	39.11111	10
>>END_MODULE