FastQCFastQC Report
Thu 26 May 2016
SRR1545944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545944_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences870220
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77430.8897749994254326No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35730.41058582887086026No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG408.309144E-944.0000041
TTGGCTA207.855426E-444.00000442
GATCGGT207.855426E-444.0000049
TTTACGG408.309144E-944.0000042
ACGTTAG207.855426E-444.0000041
ACCGCTT207.855426E-444.00000419
ATAATGC207.855426E-444.00000432
TAGGTGA207.855426E-444.00000416
TAGGTCG207.855426E-444.0000041
GACGTGA207.855426E-444.00000416
GAACTCA207.855426E-444.00000421
CGCATGA207.855426E-444.00000417
CGCATCG207.855426E-444.00000421
TCTTAAT207.855426E-444.00000422
CCGTGTA207.855426E-444.00000436
CACGCGA207.855426E-444.00000437
ATTAACG207.855426E-444.00000413
CGACATA207.855426E-444.00000413
TGACGTG207.855426E-444.00000415
CGCGACA207.855426E-444.00000439
Produced by FastQC (version 0.11.5)