##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545944_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	870220
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.498018891774493	31.0	31.0	33.0	30.0	34.0
2	31.88251246811151	31.0	31.0	34.0	30.0	34.0
3	31.896923766403898	31.0	31.0	34.0	30.0	34.0
4	35.66934913010503	37.0	35.0	37.0	33.0	37.0
5	35.59975063776976	37.0	35.0	37.0	33.0	37.0
6	35.64340166854359	37.0	35.0	37.0	33.0	37.0
7	35.98164027487302	37.0	35.0	37.0	35.0	37.0
8	35.98242628300889	37.0	35.0	37.0	35.0	37.0
9	37.79713405805428	39.0	38.0	39.0	35.0	39.0
10	37.32500861851026	39.0	37.0	39.0	34.0	39.0
11	36.94098733653559	39.0	37.0	39.0	33.0	39.0
12	35.58501528349153	35.0	35.0	39.0	32.0	39.0
13	34.964291788283425	35.0	35.0	39.0	30.0	39.0
14	35.81597641975593	36.0	35.0	40.0	30.0	41.0
15	36.21233940842546	36.0	35.0	41.0	31.0	41.0
16	36.5143515432879	36.0	35.0	41.0	32.0	41.0
17	36.47675760152605	36.0	35.0	40.0	32.0	41.0
18	36.373622762060165	36.0	35.0	40.0	32.0	41.0
19	36.18930155592839	36.0	35.0	40.0	32.0	41.0
20	35.900474592631745	35.0	35.0	40.0	32.0	41.0
21	35.708698949690884	35.0	35.0	40.0	31.0	41.0
22	35.58219645606858	35.0	35.0	40.0	31.0	41.0
23	35.57263335708212	35.0	34.0	40.0	31.0	41.0
24	35.54989198133805	35.0	34.0	40.0	31.0	41.0
25	35.5185194548505	35.0	35.0	40.0	31.0	41.0
26	35.27027188527039	35.0	34.0	40.0	31.0	41.0
27	35.23057962354347	35.0	34.0	40.0	30.0	41.0
28	35.299307071774955	35.0	34.0	40.0	30.0	41.0
29	35.32094183080141	35.0	35.0	40.0	31.0	41.0
30	35.21115809795224	35.0	35.0	40.0	30.0	41.0
31	34.862807106248994	35.0	34.0	40.0	29.0	41.0
32	34.48571395739008	35.0	34.0	40.0	27.0	41.0
33	34.14181126611661	35.0	34.0	40.0	25.0	41.0
34	33.85501942037646	35.0	34.0	40.0	23.0	41.0
35	33.56401025028154	35.0	33.0	40.0	23.0	41.0
36	33.307872721840454	35.0	33.0	39.0	21.0	41.0
37	33.19090230056767	35.0	33.0	39.0	20.0	41.0
38	33.15499873595183	35.0	33.0	39.0	21.0	41.0
39	33.136195444830044	35.0	33.0	39.0	20.0	41.0
40	32.92907310794971	35.0	33.0	39.0	18.0	41.0
41	32.95623175748661	35.0	33.0	39.0	18.0	41.0
42	32.89627680356691	35.0	33.0	39.0	18.0	41.0
43	32.776357702649904	35.0	33.0	39.0	18.0	41.0
44	32.643402817678286	35.0	32.0	39.0	18.0	41.0
45	32.529495989519894	35.0	32.0	38.0	18.0	41.0
46	32.51517892027304	35.0	32.0	38.0	18.0	41.0
47	32.47616349888534	35.0	32.0	38.0	18.0	41.0
48	32.43168164372228	35.0	32.0	38.0	18.0	41.0
49	32.412371584197096	35.0	32.0	38.0	18.0	40.0
50	32.18152076486406	35.0	32.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	5.0
14	5.0
15	16.0
16	87.0
17	197.0
18	458.0
19	1008.0
20	1771.0
21	2848.0
22	4391.0
23	6483.0
24	9786.0
25	14392.0
26	21257.0
27	25573.0
28	25432.0
29	23980.0
30	25451.0
31	29338.0
32	36265.0
33	48542.0
34	97477.0
35	184340.0
36	44066.0
37	50478.0
38	77076.0
39	139495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.052952127048332	23.177817103720898	27.809174691457333	17.960056077773437
2	33.53680678449128	23.574038748822137	30.212704833260556	12.67644963342603
3	26.694858771345174	23.630806003079684	37.06177748155639	12.612557744018755
4	24.051044563443728	26.1497092689205	35.92666222334582	13.872583944289948
5	21.28300889430259	30.776240490910347	34.62250925053435	13.318241364252717
6	17.94212957642895	40.412999011744155	31.63855117096826	10.006320240858633
7	83.21263588517847	4.025418859598722	11.468364321665787	1.2935809335570316
8	85.61731516168325	2.5640642596125116	10.430695686148331	1.3879248925559053
9	83.28514628484751	3.2866401599595503	11.734733745489647	1.6934798097032935
10	60.23097607501552	16.434235021029163	15.22201282434327	8.112776079612052
11	56.03054400036772	14.846935257750912	20.292684608489807	8.829836133391558
12	49.382455011376436	18.422352968214934	22.706327135666843	9.488864884741789
13	18.642067523155063	48.24262830088943	23.158856381145	9.956447794810508
14	11.500884833720209	48.77065569626072	28.98002803888672	10.748431431132358
15	9.54953919698467	19.486221874928177	60.38254694215256	10.58169198593459
16	10.935625474018064	12.653122198984166	58.44820849900025	17.96304382799752
17	11.577302291374595	13.56898255613523	31.994208361104086	42.85950679138609
18	18.98186665440923	19.6341155110202	40.44437038909701	20.939647445473557
19	28.135528946703133	21.201994897841924	29.617912711728067	21.044563443726876
20	31.50076992025005	19.307991082714715	28.167704718347085	21.023534278688146
21	19.363724115740848	27.161752200592954	32.48856610971938	20.985957573946816
22	21.33012341706695	22.478568637815723	27.87272184045414	28.31858610466319
23	16.033187010181333	29.637792742065226	27.508331226586378	26.82068902116706
24	17.594975983084737	20.24890257636	46.92388131736802	15.23224012318724
25	13.499344993220106	22.03178506584542	43.61862517524304	20.850244765691432
26	13.990714991611316	34.3694697892487	31.316678541058586	20.323136678081404
27	14.06345521822068	35.631909172393186	33.143457976143964	17.16117763324217
28	11.487210130771528	26.68566569373262	46.675668221828964	15.15145595366689
29	11.65452414332008	21.707384339592288	44.83935096872055	21.79874054836708
30	15.957344119877732	29.956792535220977	36.34873939923238	17.737123945668912
31	30.059984831421936	24.157339523338926	27.982579117924207	17.80009652731493
32	30.504814874399578	24.521615223736525	29.96437682425134	15.009193077612556
33	28.50980211900439	26.492151409988278	27.311714279147804	17.68633219185953
34	17.435361173036704	26.595458619659397	31.059157454436807	24.910022752867093
35	18.445220748776173	24.410493898094735	33.73089563558641	23.41338971754269
36	33.628737560616855	21.098342947760337	28.47567281836777	16.79724667325504
37	19.381190963204705	31.28875456781044	31.56718990600078	17.762864562984074
38	19.097469605387143	32.18151731745995	26.13626439291214	22.584748684240765
39	18.71699110569741	30.980441727379283	29.835099170324746	20.46746799659856
40	23.264346946749097	24.989083220335086	26.431132357334924	25.315437475580886
41	15.292684608489807	22.134287881225436	29.82119464043575	32.751832869849004
42	22.177725172944772	23.762267012939255	25.69327296545701	28.366734848658957
43	23.102778607708395	24.98287789294661	26.192571993289054	25.72177150605594
44	18.215968375813013	30.999402449955184	29.252602790099058	21.53202638413275
45	16.37792742065225	39.764427386178205	22.144170439658936	21.713474753510607
46	22.28516926754154	32.41927328721473	26.79540805773253	18.500149387511204
47	22.081197858012917	26.318746983521407	27.719772011675207	23.880283146790468
48	24.070464939900255	21.829192617958675	32.15324860380134	21.94709383833973
49	20.90770150076992	20.593642986830915	34.99344993220105	23.505205580198112
50	19.219737537634163	33.212406058238145	26.807588885569167	20.760267518558525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401.0
1	627.5
2	854.0
3	6784.0
4	12714.0
5	9841.5
6	6969.0
7	7714.5
8	8460.0
9	9352.5
10	10245.0
11	10201.0
12	10157.0
13	9505.5
14	8854.0
15	7939.0
16	7024.0
17	6369.5
18	5715.0
19	5057.0
20	4399.0
21	3883.0
22	3367.0
23	3369.5
24	3372.0
25	3439.0
26	3506.0
27	3903.0
28	4300.0
29	4872.0
30	5444.0
31	7222.5
32	9001.0
33	11277.5
34	13554.0
35	14854.0
36	16154.0
37	16965.0
38	17776.0
39	28137.0
40	38498.0
41	56851.0
42	75204.0
43	89126.0
44	103048.0
45	106846.0
46	110644.0
47	96423.0
48	82202.0
49	77720.5
50	73239.0
51	68436.0
52	63633.0
53	54185.5
54	44738.0
55	39720.5
56	34703.0
57	30222.0
58	25741.0
59	23011.5
60	20282.0
61	17701.5
62	15121.0
63	12771.5
64	10422.0
65	9026.0
66	7630.0
67	6328.5
68	5027.0
69	4007.0
70	2987.0
71	2757.0
72	2527.0
73	1845.5
74	1164.0
75	857.5
76	551.0
77	428.5
78	306.0
79	218.5
80	131.0
81	113.0
82	95.0
83	55.0
84	15.0
85	9.5
86	4.0
87	5.5
88	7.0
89	18.0
90	29.0
91	17.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	870220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.594222498786223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.92262747160237	16.052101903637535
2	6.790620773952533	2.661138686994117
3	2.2240911661579994	1.3073801150185427
4	1.0639870882030722	0.8339199896834673
5	0.7071941120673982	0.692845939084008
6	0.47534964404749613	0.5588464014111284
7	0.3665325670757828	0.5027344470633922
8	0.318600271546189	0.49941796870797894
9	0.26898597202792845	0.47435138864707654
>10	3.0269782667790803	14.569219021495158
>50	1.451541459199131	20.823289498106725
>100	1.3793490180975028	39.37331993878983
>500	0.002958706602525746	0.3393771937712404
>1k	5.917413205051491E-4	0.41427902744948475
>5k	5.917413205051491E-4	0.8977784801403192
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7743	0.8897749994254326	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3573	0.41058582887086026	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05515846567534646	0.0
2	0.0	0.0	0.0	0.22120843005217072	0.0
3	0.0	0.0	0.0	0.30532509020707405	0.0
4	0.0	0.0	0.0	0.4446002160373239	0.0
5	0.0	0.0	0.0	0.7295856220266139	0.0
6	0.0	0.0	0.0	0.9773390636850452	0.0
7	0.0	0.0	0.0	1.1343108639194686	0.0
8	0.0	0.0	0.0	1.5384615384615385	0.0
9	0.0	0.0	0.0	1.6863551745535612	0.0
10	0.0	0.0	0.0	2.0197191514789363	0.0
11	0.0	0.0	0.0	2.2679322470179955	0.0
12	0.0	0.0	0.0	2.5151111213256416	0.0
13	0.0	0.0	0.0	2.6126726574889108	0.0
14	0.0	0.0	0.0	2.6463423042449037	0.0
15	0.0	0.0	0.0	2.7071315299579415	0.0
16	0.0	0.0	0.0	2.8511181080646275	0.0
17	0.0	0.0	0.0	3.0045275907241846	0.0
18	0.0	0.0	0.0	3.292615660407713	0.0
19	0.0	0.0	0.0	3.382822734480936	0.0
20	0.0	0.0	0.0	3.4727999816138446	0.0
21	0.0	0.0	0.0	3.601847808600124	0.0
22	0.0	0.0	0.0	3.7151524901748982	0.0
23	0.0	0.0	0.0	3.866148789961159	0.0
24	0.0	0.0	0.0	3.9714095286249456	0.0
25	0.0	0.0	0.0	4.050584909563099	0.0
26	0.0	0.0	0.0	4.137114752591299	0.0
27	0.0	0.0	0.0	4.215945393118981	0.0
28	0.0	0.0	0.0	4.291788283422583	0.0
29	0.0	0.0	0.0	4.388430511824596	0.0
30	0.0	0.0	0.0	4.527360897244375	0.0
31	0.0	0.0	0.0	4.657902599342695	0.0
32	0.0	0.0	0.0	4.754544827744708	0.0
33	0.0	0.0	0.0	4.847394911631541	0.0
34	0.0	0.0	0.0	4.945531015145596	0.0
35	0.0	0.0	0.0	5.1106616717611635	0.0
36	0.0	0.0	0.0	5.218105766357932	0.0
37	1.149134701569718E-4	0.0	0.0	5.332099928753649	0.0
38	1.149134701569718E-4	0.0	0.0	5.43827997517869	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	40	8.309144E-9	44.000004	1
TTGGCTA	20	7.855426E-4	44.000004	42
GATCGGT	20	7.855426E-4	44.000004	9
TTTACGG	40	8.309144E-9	44.000004	2
ACGTTAG	20	7.855426E-4	44.000004	1
ACCGCTT	20	7.855426E-4	44.000004	19
ATAATGC	20	7.855426E-4	44.000004	32
TAGGTGA	20	7.855426E-4	44.000004	16
TAGGTCG	20	7.855426E-4	44.000004	1
GACGTGA	20	7.855426E-4	44.000004	16
GAACTCA	20	7.855426E-4	44.000004	21
CGCATGA	20	7.855426E-4	44.000004	17
CGCATCG	20	7.855426E-4	44.000004	21
TCTTAAT	20	7.855426E-4	44.000004	22
CCGTGTA	20	7.855426E-4	44.000004	36
CACGCGA	20	7.855426E-4	44.000004	37
ATTAACG	20	7.855426E-4	44.000004	13
CGACATA	20	7.855426E-4	44.000004	13
TGACGTG	20	7.855426E-4	44.000004	15
CGCGACA	20	7.855426E-4	44.000004	39
>>END_MODULE