FastQCFastQC Report
Thu 26 May 2016
SRR1545943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545943_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1498616
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148240.9891793494797867No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20240.13505794679891314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.857248E-444.0000041
CGCTATT207.857248E-444.00000442
CGTACTA207.857248E-444.00000441
TCGAACA207.857248E-444.00000441
CTATGCG351.4464786E-744.01
CGGCATA254.4434895E-544.020
ACTACGG600.044.02
AGCGATC254.4434895E-544.015
CGTCGAC254.4434895E-544.019
TGCGTTG351.4464786E-744.01
CGTTTTT111350.043.288731
CGGTCTA1800.041.55555731
TCACGAC1750.041.48571825
ATTAACG700.040.8571431
ACGGTCT1850.040.43243430
GCCGATT557.8216544E-1140.09
GCGATAC557.8216544E-1140.09
ATACCGG557.8216544E-1140.02
CGCATGG1000.039.6000022
TCGTAAG501.3478711E-939.6000021
Produced by FastQC (version 0.11.5)