##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545943_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1498616
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473857212254508	31.0	31.0	33.0	30.0	34.0
2	31.87222343815894	31.0	31.0	34.0	30.0	34.0
3	31.920146988955143	31.0	31.0	34.0	30.0	34.0
4	35.695567777202434	37.0	35.0	37.0	33.0	37.0
5	35.61830982720056	37.0	35.0	37.0	33.0	37.0
6	35.67143818029435	37.0	35.0	37.0	33.0	37.0
7	35.97335675049512	37.0	35.0	37.0	35.0	37.0
8	35.968739823944226	37.0	35.0	37.0	35.0	37.0
9	37.76623297762736	39.0	38.0	39.0	35.0	39.0
10	37.22853219236949	39.0	37.0	39.0	34.0	39.0
11	36.896770086533174	39.0	37.0	39.0	33.0	39.0
12	36.23015969401101	38.0	35.0	39.0	32.0	39.0
13	35.931456090152516	38.0	35.0	39.0	31.0	39.0
14	36.928323199538774	39.0	35.0	41.0	31.0	41.0
15	37.1324508746737	39.0	35.0	41.0	32.0	41.0
16	37.29778809248	39.0	35.0	41.0	32.0	41.0
17	37.238158407490644	39.0	35.0	41.0	32.0	41.0
18	37.11682846039279	39.0	35.0	41.0	32.0	41.0
19	36.98698198871492	38.0	35.0	41.0	32.0	41.0
20	36.753049480320506	38.0	35.0	41.0	32.0	41.0
21	36.61122662509943	38.0	35.0	40.0	32.0	41.0
22	36.5252346164728	38.0	35.0	40.0	31.0	41.0
23	36.44099355672167	38.0	35.0	40.0	31.0	41.0
24	36.47431630250845	38.0	35.0	40.0	31.0	41.0
25	36.43998796222648	38.0	35.0	40.0	31.0	41.0
26	36.205077217913065	38.0	35.0	40.0	31.0	41.0
27	36.18139603474139	37.0	35.0	40.0	31.0	41.0
28	36.17353077773092	37.0	35.0	40.0	31.0	41.0
29	36.116278619739816	37.0	35.0	40.0	31.0	41.0
30	36.00806143802015	37.0	35.0	40.0	30.0	41.0
31	35.778267414734664	37.0	35.0	40.0	30.0	41.0
32	35.498459912345794	37.0	34.0	40.0	29.0	41.0
33	35.20518465037074	37.0	34.0	40.0	27.0	41.0
34	34.926051103151174	37.0	34.0	40.0	25.0	41.0
35	34.65792304366162	37.0	34.0	40.0	23.0	41.0
36	34.47585572288031	37.0	34.0	40.0	23.0	41.0
37	34.36360615394471	37.0	34.0	40.0	23.0	41.0
38	34.2935802100071	37.0	33.0	40.0	23.0	41.0
39	34.19488448008029	36.0	33.0	40.0	22.0	41.0
40	34.02851497648497	36.0	33.0	40.0	21.0	41.0
41	33.988327229924145	36.0	33.0	40.0	21.0	41.0
42	33.97243523357551	36.0	33.0	40.0	21.0	41.0
43	33.8635581096158	36.0	33.0	40.0	21.0	41.0
44	33.752102606671755	36.0	33.0	40.0	20.0	41.0
45	33.65031535763664	35.0	33.0	40.0	20.0	41.0
46	33.6132057845372	35.0	33.0	40.0	20.0	41.0
47	33.54985399862273	35.0	33.0	40.0	20.0	41.0
48	33.451323754717684	35.0	33.0	40.0	20.0	41.0
49	33.39403022522114	36.0	33.0	40.0	19.0	41.0
50	33.244099222215695	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	7.0
13	10.0
14	7.0
15	37.0
16	114.0
17	315.0
18	725.0
19	1442.0
20	2631.0
21	4165.0
22	6465.0
23	9570.0
24	14146.0
25	21294.0
26	30859.0
27	37340.0
28	36976.0
29	36842.0
30	39139.0
31	45375.0
32	54831.0
33	71025.0
34	126185.0
35	206378.0
36	96119.0
37	123088.0
38	189151.0
39	344377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.30646409754067	22.640022527451993	27.081587277861708	16.971926097145634
2	33.68754904525242	24.413792459175664	29.213821285773005	12.684837209798907
3	27.740128224975578	24.498604045332492	34.969531888088746	12.791735841603186
4	24.736356745156865	26.674611775131186	34.7462592151692	13.842772264542752
5	22.187938738142392	30.70633170872325	33.98135346212772	13.124376091006635
6	19.360730167034117	40.235857617962175	30.951357786117324	9.452054428886386
7	85.57829357220263	3.074703593181976	10.09217838325495	1.2548244513604554
8	87.05565668590219	2.0282046901941526	9.72830932006598	1.1878293038376742
9	83.36571876985165	3.8595610883641975	10.889714243008216	1.8850058987759373
10	45.499314033748476	29.08116555541913	16.03579569416048	9.383724716671917
11	39.93858333288848	20.233402018929468	27.302190821397875	12.52582382678418
12	36.63980632797194	20.741604253524585	28.848684386126934	13.769905032376538
13	21.648707874465504	34.322067827915895	29.651091407004866	14.37813289061374
14	16.348150560250257	36.377898007228005	31.466032659467135	15.807918773054604
15	14.971013254896517	23.397387989985425	45.916432228135825	15.71516652698223
16	17.608913824488727	19.032427252878655	44.38602016794162	18.972638754690998
17	17.691723563608022	19.57406033300058	32.16354289557832	30.570673207813076
18	21.57951069520144	22.703481078541802	36.122328868769586	19.594679357487173
19	26.564043090424764	23.846802649911652	29.59237056057055	19.99678369909303
20	28.319529485872298	22.976332829757588	29.271407752219382	19.432729932150732
21	22.15924559727108	26.082598877898004	32.251023611118526	19.507131913712385
22	22.569490783496242	22.404271674665157	30.43174502340826	24.59449251843034
23	19.10282554036524	27.895404826853575	30.272264542751447	22.729505090029733
24	19.019815616542196	23.579689526870126	39.66553139696894	17.73496345961874
25	17.92400454819647	25.228944572859223	36.584488621501436	20.262562257442866
26	17.66116203216835	31.061125732008733	30.966972193010083	20.310740042812835
27	17.30303159715364	30.7043965899203	32.84250268247503	19.15006913045103
28	16.146297650632317	26.129909196218374	39.32394956413117	18.399843589018133
29	16.439568241630944	23.465984615138233	38.93212137065132	21.1623257725795
30	19.16628409145505	27.970006993118986	34.83167135543728	18.032037559988684
31	27.28711024038179	25.446278432900755	28.811850400636317	18.454760926081132
32	27.271228920550694	25.17075755230159	29.446502639768962	18.111510887378753
33	25.750759367309573	26.04569816417281	28.42649484591116	19.77704762260646
34	19.98210348748445	26.355050259706285	30.430277002247408	23.232569250561852
35	20.151259562156014	25.72413480171038	32.66647359964127	21.458132036492337
36	27.97354358955196	25.36240104202811	27.748068884891126	18.9159864835288
37	20.21318336385038	30.529635343543642	29.789485765532998	19.46769552707298
38	20.7865790836345	30.360412540637494	27.37946211704666	21.473546258681345
39	20.772966523779274	29.835728432100016	28.046277365249	21.345027678871705
40	23.68685507161274	26.01567045860981	28.08497974130798	22.212494728469466
41	19.043237226881335	24.379627603068432	29.523573750713993	27.05356141933624
42	21.959127621752337	25.11850934462197	26.94319291933357	25.97917011429212
43	21.85903526987567	26.40282767566875	27.361178580770524	24.376958473685054
44	19.871134433370525	29.224631259775684	29.081098827184547	21.823135479669244
45	18.386965039743338	34.07143657881672	25.578400337377953	21.963198044061986
46	21.914686617519095	30.884829736236636	27.45319681626247	19.747286829981796
47	21.537672092116996	26.68635594441805	29.265001841699274	22.510970121765684
48	22.392060407736206	24.82477165598125	31.63565583178079	21.147512104501754
49	21.109877380196128	24.973175249697054	31.9157142323317	22.00123313777512
50	19.903831268316903	30.320775969294335	29.35595242543787	20.419440336950892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	344.0
1	1074.0
2	1804.0
3	14159.5
4	26515.0
5	19291.5
6	12068.0
7	12147.0
8	12226.0
9	12548.0
10	12870.0
11	12737.0
12	12604.0
13	11892.0
14	11180.0
15	10425.5
16	9671.0
17	9232.5
18	8794.0
19	7746.0
20	6698.0
21	6973.5
22	7249.0
23	7213.5
24	7178.0
25	8550.5
26	9923.0
27	11702.5
28	13482.0
29	15811.5
30	18141.0
31	24168.0
32	30195.0
33	32838.0
34	35481.0
35	39498.5
36	43516.0
37	46583.5
38	49651.0
39	61280.0
40	72909.0
41	95301.5
42	117694.0
43	128531.5
44	139369.0
45	141790.5
46	144212.0
47	132269.0
48	120326.0
49	117359.0
50	114392.0
51	106509.0
52	98626.0
53	88934.0
54	79242.0
55	71796.0
56	64350.0
57	58470.5
58	52591.0
59	47035.5
60	41480.0
61	39357.5
62	37235.0
63	32581.0
64	27927.0
65	24022.0
66	20117.0
67	16732.5
68	13348.0
69	11503.5
70	9659.0
71	8282.0
72	6905.0
73	5566.0
74	4227.0
75	3115.5
76	2004.0
77	1603.0
78	1202.0
79	910.5
80	619.0
81	469.5
82	320.0
83	250.5
84	181.0
85	115.5
86	50.0
87	37.5
88	25.0
89	15.0
90	5.0
91	5.5
92	6.0
93	3.0
94	0.0
95	1.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1498616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.168310748024602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36865241932688	16.589319873162413
2	7.015708163741372	2.970213810550608
3	2.647246315538804	1.6811319790166577
4	1.4793162652329834	1.2525850558823592
5	1.0465164072212176	1.1076492255482497
6	0.8086014247697351	1.0270035738492715
7	0.6000418200996693	0.8891310196776051
8	0.48786709974828046	0.8261857896967293
9	0.4244448927079849	0.8086303245829127
>10	4.754902152360087	23.626781640721052
>50	1.5255353092488169	23.132862516413145
>100	0.8379742306496988	24.61209964111091
>500	0.002554799483684543	0.3374641256658498
>1k	3.1934993546056786E-4	0.13682439710490385
>5k	0.0	0.0
>10k+	3.1934993546056786E-4	1.0021170270173394
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14824	0.9891793494797867	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2024	0.13505794679891314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022954512697048478	0.0
2	0.0	0.0	0.0	0.08814799788604953	0.0
3	0.0	0.0	0.0	0.12584945042625997	0.0
4	0.0	0.0	0.0	0.19638119438201648	0.0
5	0.0	0.0	0.0	0.36960769136323113	0.0
6	0.0	0.0	0.0	0.5088695169409642	0.0
7	0.0	0.0	0.0	0.6167023440294245	0.0
8	0.0	0.0	0.0	0.8904882905293952	0.0
9	0.0	0.0	0.0	1.0083303528055219	0.0
10	0.0	0.0	0.0	1.256159016052144	0.0
11	0.0	0.0	0.0	1.4379267270601674	0.0
12	0.0	0.0	0.0	1.6146230922397733	0.0
13	0.0	0.0	0.0	1.6786821974408388	0.0
14	0.0	0.0	0.0	1.7038387418791738	0.0
15	0.0	0.0	0.0	1.7444095085065154	0.0
16	0.0	0.0	0.0	1.8288874534904205	0.0
17	0.0	0.0	0.0	1.9402568770118562	0.0
18	0.0	0.0	0.0	2.1004713682491043	0.0
19	0.0	0.0	0.0	2.1734720568844854	0.0
20	0.0	0.0	0.0	2.257883273633806	0.0
21	0.0	0.0	0.0	2.36111185253594	0.0
22	0.0	0.0	0.0	2.4792208277504044	0.0
23	0.0	0.0	0.0	2.613478035734304	0.0
24	0.0	0.0	0.0	2.71163526880802	0.0
25	0.0	0.0	0.0	2.794711920865652	0.0
26	0.0	0.0	0.0	2.8765207364661793	0.0
27	0.0	0.0	0.0	2.9641349084755535	0.0
28	0.0	0.0	0.0	3.056953882782514	0.0
29	0.0	0.0	0.0	3.160716287561323	0.0
30	0.0	0.0	0.0	3.2979095378669387	0.0
31	0.0	0.0	0.0	3.4128822860559342	0.0
32	0.0	0.0	0.0	3.5227169601819277	0.0
33	0.0	0.0	0.0	3.6378231648400923	0.0
34	0.0	0.0	0.0	3.7679432222797566	0.0
35	0.0	0.0	0.0	3.9217518029968983	0.0
36	0.0	0.0	0.0	4.040928429964714	0.0
37	6.672823458444325E-5	0.0	0.0	4.162173632204648	0.0
38	6.672823458444325E-5	0.0	0.0	4.299700523683185	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.857248E-4	44.000004	1
CGCTATT	20	7.857248E-4	44.000004	42
CGTACTA	20	7.857248E-4	44.000004	41
TCGAACA	20	7.857248E-4	44.000004	41
CTATGCG	35	1.4464786E-7	44.0	1
CGGCATA	25	4.4434895E-5	44.0	20
ACTACGG	60	0.0	44.0	2
AGCGATC	25	4.4434895E-5	44.0	15
CGTCGAC	25	4.4434895E-5	44.0	19
TGCGTTG	35	1.4464786E-7	44.0	1
CGTTTTT	11135	0.0	43.28873	1
CGGTCTA	180	0.0	41.555557	31
TCACGAC	175	0.0	41.485718	25
ATTAACG	70	0.0	40.857143	1
ACGGTCT	185	0.0	40.432434	30
GCCGATT	55	7.8216544E-11	40.0	9
GCGATAC	55	7.8216544E-11	40.0	9
ATACCGG	55	7.8216544E-11	40.0	2
CGCATGG	100	0.0	39.600002	2
TCGTAAG	50	1.3478711E-9	39.600002	1
>>END_MODULE