FastQCFastQC Report
Thu 26 May 2016
SRR1545942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545942_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2280082
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131000.5745407402014489No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47100.206571518041895No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG207.8581134E-444.029
TAAGTCG408.3182385E-944.01
CGACATA351.4469151E-744.023
ATCGTTC207.8581134E-444.025
ACCTAGT351.4469151E-744.029
GCGTAAG1900.044.01
AATCGTC207.8581134E-444.021
TACCGTA207.8581134E-444.028
CGTAAGG3300.042.6666682
CGTTTTT94000.042.5723421
CGACGGT5850.041.3675228
GGTACGC650.040.6153838
CGTTTAG1750.040.22857330
GGCGATA6950.040.201448
ACGACGG6050.040.027
CCGCTCG2150.039.90697519
CGGTCTA6050.039.63636431
TAATGCG1000.039.61
AATACCG501.3496901E-939.638
ACCGCTC2400.039.41666418
Produced by FastQC (version 0.11.5)