##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545942_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2280082
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41557979055139	31.0	31.0	33.0	30.0	34.0
2	31.80649117005441	31.0	31.0	34.0	30.0	34.0
3	31.924638675275713	31.0	31.0	34.0	30.0	34.0
4	35.6657001809584	37.0	35.0	37.0	33.0	37.0
5	35.57782132396993	37.0	35.0	37.0	33.0	37.0
6	35.63644333844134	37.0	35.0	37.0	33.0	37.0
7	35.93655359763377	37.0	35.0	37.0	35.0	37.0
8	35.91561970139671	37.0	35.0	37.0	35.0	37.0
9	37.690826908856785	39.0	38.0	39.0	35.0	39.0
10	37.15210067006362	39.0	37.0	39.0	33.0	39.0
11	36.82547645216268	39.0	35.0	39.0	33.0	39.0
12	35.91878055262925	37.0	35.0	39.0	32.0	39.0
13	35.547260580979106	37.0	35.0	39.0	31.0	39.0
14	36.435351447886525	38.0	35.0	41.0	31.0	41.0
15	36.69361847512502	38.0	35.0	41.0	31.0	41.0
16	36.89039780148258	38.0	35.0	41.0	32.0	41.0
17	36.83971716806676	38.0	35.0	41.0	32.0	41.0
18	36.79204388263229	38.0	35.0	40.0	32.0	41.0
19	36.71124547275054	38.0	35.0	40.0	32.0	41.0
20	36.52634335080931	37.0	35.0	40.0	32.0	41.0
21	36.35396753274663	37.0	35.0	40.0	31.0	41.0
22	36.24987039939791	37.0	35.0	40.0	31.0	41.0
23	36.19144969347594	37.0	35.0	40.0	31.0	41.0
24	36.24634991197685	37.0	35.0	40.0	31.0	41.0
25	36.2094218541263	37.0	35.0	40.0	31.0	41.0
26	35.960294410464186	37.0	35.0	40.0	31.0	41.0
27	35.94013724067818	37.0	35.0	40.0	30.0	41.0
28	35.92777408882663	37.0	35.0	40.0	30.0	41.0
29	35.87348656758836	36.0	35.0	40.0	30.0	41.0
30	35.78378979352497	36.0	35.0	40.0	30.0	41.0
31	35.57827832507778	36.0	34.0	40.0	30.0	41.0
32	35.422745322317354	36.0	34.0	40.0	29.0	41.0
33	35.24801695728487	36.0	34.0	40.0	29.0	41.0
34	35.06765151428765	36.0	34.0	40.0	27.0	41.0
35	34.906675724820424	36.0	34.0	40.0	27.0	41.0
36	34.723933612913925	36.0	34.0	40.0	25.0	41.0
37	34.64483821195904	36.0	34.0	40.0	25.0	41.0
38	34.55704268530693	36.0	34.0	40.0	25.0	41.0
39	34.478240256271484	36.0	34.0	40.0	24.0	41.0
40	34.2879804322827	36.0	33.0	40.0	23.0	41.0
41	34.26255020652766	36.0	34.0	40.0	23.0	41.0
42	34.23475383780057	35.0	33.0	40.0	23.0	41.0
43	34.14793590756824	35.0	33.0	40.0	23.0	41.0
44	34.00104294494672	35.0	33.0	40.0	23.0	41.0
45	33.88504536240363	35.0	33.0	40.0	23.0	41.0
46	33.861632607950064	35.0	33.0	40.0	23.0	41.0
47	33.795031494481336	35.0	33.0	40.0	23.0	41.0
48	33.70895783572696	35.0	33.0	39.0	23.0	41.0
49	33.663620869775734	35.0	33.0	39.0	23.0	41.0
50	33.450568005887504	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	7.0
13	12.0
14	36.0
15	67.0
16	187.0
17	459.0
18	1022.0
19	2152.0
20	3649.0
21	5980.0
22	9079.0
23	13152.0
24	18799.0
25	27413.0
26	38353.0
27	46854.0
28	51514.0
29	55183.0
30	62164.0
31	74623.0
32	91820.0
33	120632.0
34	219610.0
35	365205.0
36	147564.0
37	187437.0
38	281284.0
39	455822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.78476563562188	22.41599205642604	28.02706218460564	13.772180123346441
2	32.20314006250653	24.304082046172024	30.392415711364766	13.100362179956685
3	29.931993673911727	24.5437664084011	32.470279577664314	13.053960340022858
4	26.24436314132562	26.626323088380154	32.23962997822008	14.889683792074146
5	21.84127588393751	32.28186530133566	31.023708796438022	14.853150018288815
6	20.45163287987011	41.06637392865695	28.231440799058984	10.250552392413956
7	87.23809055990091	3.4410165950171967	7.483064205585589	1.8378286394962986
8	88.28866681110593	2.3338634312274733	7.2673263505435335	2.110143407123077
9	84.5486697408251	3.8021878160522298	9.024938576770484	2.624203866352175
10	52.22991980112997	22.72856853393869	14.072125476189015	10.969386188742334
11	46.088342436807096	20.6304422384809	19.843365282476682	13.437850042235322
12	40.514683243848246	20.44294898165943	24.860684835019093	14.18168293947323
13	22.710279718010142	37.475801308900294	25.465706935101455	14.348212037988105
14	16.81237780044753	39.904442033225116	28.73471217263239	14.548467993694963
15	15.479004702462456	21.91491358644119	47.71516989301262	14.890911818083735
16	17.479502930157775	17.606252757576264	45.61616643611941	19.29807787614656
17	18.01141362459771	17.897865076782328	28.749974781608735	35.340746517011226
18	22.47765650533621	22.59497684732391	34.044214199313885	20.883152448025992
19	28.870584478979268	23.576081912843485	26.534089563445523	21.019244044731725
20	30.830250841855687	23.391483288758913	24.458462458806306	21.319803410579095
21	23.677657207065362	26.933768171495586	28.832691104969033	20.555883516470022
22	23.34288854523653	22.87856313939586	26.52720384617746	27.25134446919014
23	19.8063052118301	29.138206432926534	26.256950407923924	24.79853794731944
24	20.79999754394798	23.393983198849867	37.22181921527384	18.584200041928316
25	19.544165516854218	22.813521618959317	35.22987331157388	22.412439552612582
26	18.033474234698577	30.936738240115925	27.937504002049053	23.092283523136448
27	19.13545214601931	30.72069337857147	29.876951793838995	20.266902681570222
28	16.819745956505074	26.166865928506077	38.19700344110431	18.81638467388454
29	17.459152784855984	24.086677584402665	36.76784431437115	21.6863253163702
30	20.587505186216987	27.416908690126057	32.18897390532446	19.806612218332496
31	29.789761947158038	23.786556799273008	25.413910552339786	21.009770701229165
32	29.00246570079497	24.037688118234342	27.373357624857352	19.58648855611333
33	27.18678538754308	24.108562762216444	27.558877268449116	21.145774581791358
34	19.71323838353182	25.936567193636016	29.268421048014943	25.081773374817224
35	19.529736211241524	24.65314843939823	32.034067195828925	23.78304815353132
36	30.052077074421007	22.919877443004243	26.878375426848685	20.149670055726066
37	20.85087290720246	30.122600853828942	28.217274641876916	20.80925159709168
38	21.28620812760243	30.980596311887027	24.523021540453367	23.210174020057174
39	21.12766119814989	28.437836884813795	26.637857761255958	23.79664415578036
40	24.19899810620846	23.48998851795681	26.864472418097247	25.446540957737483
41	18.769149530587057	22.94351694368887	27.367436785168252	30.919896740555824
42	22.99733079775201	24.55477478441565	24.690603232690755	27.757291185141586
43	22.748129233948603	25.173085880244656	25.973013251277806	26.105771634528935
44	21.196562228902295	27.87676057264607	28.77839481211641	22.14828238633523
45	18.7962538189416	33.35551089829226	24.325704075555176	23.52253120721097
46	22.949350067234427	28.673793310942326	27.93276733029777	20.44408929152548
47	22.18534245698181	25.569080410265947	28.146882436684294	24.09869469606795
48	22.763216410637863	23.49946186145937	30.915160068804543	22.822161659098224
49	21.853512285961646	22.542961174203384	31.875300976017527	23.728225563817443
50	20.233921411598356	29.965194234242453	27.91061900405336	21.89026535010583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	632.0
1	1652.0
2	2672.0
3	13349.5
4	24027.0
5	17318.5
6	10610.0
7	10896.5
8	11183.0
9	11471.0
10	11759.0
11	11676.5
12	11594.0
13	10973.0
14	10352.0
15	9422.0
16	8492.0
17	7868.5
18	7245.0
19	6629.0
20	6013.0
21	5983.5
22	5954.0
23	6661.5
24	7369.0
25	8423.0
26	9477.0
27	10943.5
28	12410.0
29	17295.0
30	22180.0
31	24035.0
32	25890.0
33	31829.5
34	37769.0
35	42486.5
36	47204.0
37	51496.0
38	55788.0
39	71618.0
40	87448.0
41	127123.0
42	166798.0
43	192409.0
44	218020.0
45	224525.0
46	231030.0
47	222190.5
48	213351.0
49	212370.5
50	211390.0
51	193539.0
52	175688.0
53	155865.5
54	136043.0
55	122757.0
56	109471.0
57	102679.0
58	95887.0
59	88641.5
60	81396.0
61	76181.5
62	70967.0
63	62559.5
64	54152.0
65	45773.5
66	37395.0
67	30567.0
68	23739.0
69	19476.5
70	15214.0
71	12820.5
72	10427.0
73	8394.0
74	6361.0
75	4844.5
76	3328.0
77	2429.0
78	1530.0
79	1145.5
80	761.0
81	684.5
82	608.0
83	398.5
84	189.0
85	186.5
86	184.0
87	113.5
88	43.0
89	30.0
90	17.0
91	17.0
92	17.0
93	12.0
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2280082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.559900301964152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.67053430463335	14.601172733962459
2	8.0509580778547	2.9884995852055236
3	2.8453172079649804	1.5842641112187912
4	1.4537640586052532	1.0792686396116908
5	0.9850512610529214	0.9141226598733143
6	0.7076798741597428	0.7880680746066817
7	0.5063773943757094	0.6578819768347085
8	0.4368805728089103	0.6486767900158692
9	0.38001188664723573	0.6347684456760598
>10	3.479467179440596	14.413635017139741
>50	1.086347575993676	14.792413810795546
>100	1.3854075349530968	44.44503152340963
>500	0.009331760566380833	1.0734633087080896
>1k	0.002632035031543312	0.7969705666918492
>5k	0.0	0.0
>10k+	2.3927591195848285E-4	0.5817627562500403
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13100	0.5745407402014489	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4710	0.206571518041895	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08688284017855498	0.0
2	0.0	0.0	0.0	0.33060214501057417	0.0
3	0.0	0.0	0.0	0.45270301682132486	0.0
4	0.0	0.0	0.0	0.63423157588192	0.0
5	0.0	0.0	0.0	1.0442168307981905	0.0
6	0.0	0.0	0.0	1.3938972370291947	0.0
7	0.0	0.0	0.0	1.627485327282089	0.0
8	0.0	0.0	0.0	2.2678570332119636	0.0
9	0.0	0.0	0.0	2.495261135345132	0.0
10	0.0	0.0	0.0	2.9088866102184046	0.0
11	0.0	0.0	0.0	3.2450587303439087	0.0
12	0.0	0.0	0.0	3.5649156477705626	0.0
13	0.0	0.0	0.0	3.6883322617344465	0.0
14	0.0	0.0	0.0	3.731313172070127	0.0
15	0.0	0.0	0.0	3.8081963718848706	0.0
16	0.0	0.0	0.0	3.972664141026507	0.0
17	4.385807177110297E-5	0.0	0.0	4.161560856144647	0.0
18	4.385807177110297E-5	0.0	0.0	4.479926599131084	0.0
19	4.385807177110297E-5	0.0	0.0	4.588124462190395	0.0
20	4.385807177110297E-5	0.0	0.0	4.716891760910353	0.0
21	4.385807177110297E-5	0.0	0.0	4.8925433383536205	0.0
22	4.385807177110297E-5	0.0	0.0	5.066440592926044	0.0
23	4.385807177110297E-5	0.0	0.0	5.269722755585106	0.0
24	4.385807177110297E-5	0.0	0.0	5.417743747812579	0.0
25	4.385807177110297E-5	0.0	0.0	5.535020231728508	0.0
26	4.385807177110297E-5	0.0	0.0	5.658129839189994	0.0
27	4.385807177110297E-5	0.0	0.0	5.7834323502400355	0.0
28	4.385807177110297E-5	0.0	0.0	5.917594191787839	0.0
29	4.385807177110297E-5	0.0	0.0	6.066273055091878	0.0
30	4.385807177110297E-5	0.0	0.0	6.254380324918139	0.0
31	4.385807177110297E-5	0.0	0.0	6.415339448318087	0.0
32	4.385807177110297E-5	0.0	0.0	6.552790645248724	0.0
33	4.385807177110297E-5	0.0	0.0	6.705811457658101	0.0
34	8.771614354220594E-5	0.0	0.0	6.857867392488516	0.0
35	8.771614354220594E-5	0.0	0.0	7.054176121735972	0.0
36	8.771614354220594E-5	0.0	0.0	7.206582921140556	0.0
37	8.771614354220594E-5	0.0	0.0	7.353025022784268	0.0
38	8.771614354220594E-5	0.0	0.0	7.52367678004563	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	20	7.8581134E-4	44.0	29
TAAGTCG	40	8.3182385E-9	44.0	1
CGACATA	35	1.4469151E-7	44.0	23
ATCGTTC	20	7.8581134E-4	44.0	25
ACCTAGT	35	1.4469151E-7	44.0	29
GCGTAAG	190	0.0	44.0	1
AATCGTC	20	7.8581134E-4	44.0	21
TACCGTA	20	7.8581134E-4	44.0	28
CGTAAGG	330	0.0	42.666668	2
CGTTTTT	9400	0.0	42.572342	1
CGACGGT	585	0.0	41.36752	28
GGTACGC	65	0.0	40.615383	8
CGTTTAG	175	0.0	40.228573	30
GGCGATA	695	0.0	40.20144	8
ACGACGG	605	0.0	40.0	27
CCGCTCG	215	0.0	39.906975	19
CGGTCTA	605	0.0	39.636364	31
TAATGCG	100	0.0	39.6	1
AATACCG	50	1.3496901E-9	39.6	38
ACCGCTC	240	0.0	39.416664	18
>>END_MODULE