FastQCFastQC Report
Thu 26 May 2016
SRR1545941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545941_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277678
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126200.9877293026881577No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41090.3215990257326181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG408.314601E-944.0000041
ACATTCG207.8568107E-444.00000414
ACTACGC207.8568107E-444.00000410
CGTACGT207.8568107E-444.00000435
TCGTAAT207.8568107E-444.00000435
GACGATT408.314601E-944.00000435
ATCATAA207.8568107E-444.00000421
TGTCGTG207.8568107E-444.00000413
ATCGCTA207.8568107E-444.00000410
GTACGCC207.8568107E-444.00000422
AACACGT207.8568107E-444.00000441
ATCTCGT454.802132E-1044.019
CTCACGT302.5279387E-644.044
ACCGGTT254.4431188E-544.018
GGTGTTA302.5279387E-644.08
GTCGATG254.4431188E-544.01
GATCGAT302.5279387E-644.09
CTATCCG302.5279387E-644.044
CGGCATG254.4431188E-544.019
GCTATAA302.5279387E-644.036
Produced by FastQC (version 0.11.5)