##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545941_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1277678
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.450705107233592	31.0	31.0	33.0	30.0	34.0
2	31.847233027413793	31.0	31.0	34.0	30.0	34.0
3	31.89030021648647	31.0	31.0	34.0	30.0	34.0
4	35.66129337751765	37.0	35.0	37.0	33.0	37.0
5	35.578565961063745	37.0	35.0	37.0	33.0	37.0
6	35.63257252609812	37.0	35.0	37.0	33.0	37.0
7	35.943287745425685	37.0	35.0	37.0	35.0	37.0
8	35.93233193339793	37.0	35.0	37.0	35.0	37.0
9	37.75078149580724	39.0	38.0	39.0	35.0	39.0
10	37.24827695240898	39.0	37.0	39.0	34.0	39.0
11	36.911667102352865	39.0	37.0	39.0	33.0	39.0
12	35.90775297062327	37.0	35.0	39.0	32.0	39.0
13	35.44930334560038	37.0	35.0	39.0	31.0	39.0
14	36.36246456462426	38.0	35.0	41.0	31.0	41.0
15	36.65460937732355	38.0	35.0	41.0	32.0	41.0
16	36.884636817727156	38.0	35.0	41.0	32.0	41.0
17	36.836093287980226	38.0	35.0	41.0	32.0	41.0
18	36.70718130859262	38.0	35.0	40.0	32.0	41.0
19	36.544687315583424	37.0	35.0	40.0	32.0	41.0
20	36.28235830937059	36.0	35.0	40.0	32.0	41.0
21	36.11687764835898	36.0	35.0	40.0	31.0	41.0
22	35.98345357750544	35.0	35.0	40.0	31.0	41.0
23	35.94677532210776	35.0	35.0	40.0	31.0	41.0
24	35.971432551863614	35.0	35.0	40.0	31.0	41.0
25	35.918681389207606	35.0	35.0	40.0	31.0	41.0
26	35.666899641380695	35.0	35.0	40.0	31.0	41.0
27	35.61971952244619	35.0	34.0	40.0	30.0	41.0
28	35.618571345832045	36.0	35.0	40.0	30.0	41.0
29	35.59666676580484	36.0	35.0	40.0	30.0	41.0
30	35.48162134747565	36.0	35.0	40.0	30.0	41.0
31	35.178913622994216	35.0	34.0	40.0	29.0	41.0
32	34.868564693138644	35.0	34.0	40.0	27.0	41.0
33	34.53932994072059	35.0	34.0	40.0	25.0	41.0
34	34.26145163335363	35.0	34.0	40.0	23.0	41.0
35	33.97846797080329	35.0	33.0	40.0	23.0	41.0
36	33.733479796944145	35.0	33.0	40.0	21.0	41.0
37	33.630471057653025	35.0	33.0	40.0	21.0	41.0
38	33.57121434352004	35.0	33.0	40.0	21.0	41.0
39	33.51136906168847	35.0	33.0	40.0	21.0	41.0
40	33.33426888464856	35.0	33.0	40.0	20.0	41.0
41	33.30272337787768	35.0	33.0	40.0	19.0	41.0
42	33.22962827879951	35.0	33.0	40.0	18.0	41.0
43	33.12856995267978	35.0	33.0	40.0	18.0	41.0
44	32.9858008042715	35.0	33.0	39.0	18.0	41.0
45	32.869892883809534	35.0	32.0	39.0	18.0	41.0
46	32.84030170355911	35.0	32.0	39.0	18.0	41.0
47	32.783719372173586	35.0	32.0	39.0	18.0	41.0
48	32.70270443726823	35.0	32.0	39.0	18.0	41.0
49	32.66132468431013	35.0	32.0	39.0	18.0	41.0
50	32.461556824176355	35.0	32.0	38.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	9.0
14	16.0
15	32.0
16	110.0
17	294.0
18	727.0
19	1485.0
20	2565.0
21	4146.0
22	6326.0
23	9104.0
24	13633.0
25	20251.0
26	28911.0
27	34603.0
28	35791.0
29	34673.0
30	36597.0
31	41848.0
32	50655.0
33	66796.0
34	125583.0
35	225744.0
36	76032.0
37	93598.0
38	138173.0
39	229971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.942303146802246	22.66838749669322	28.67537830345361	17.713931053050924
2	33.87246238880219	24.779326246519076	29.126196115140125	12.222015249538616
3	27.054703923836836	25.197976328934207	35.52084327976219	12.226476467466764
4	24.342439957485375	25.878742531373323	35.7594793054275	14.019338205713803
5	22.019475955600708	29.971949113939505	34.38941579959896	13.619159130860828
6	18.681389207609428	40.07418144477717	31.1429796865877	10.101449661025704
7	83.73385156510483	4.076066113684355	10.905329824885456	1.2847524963253654
8	85.83117186020264	2.3344692481204183	10.434006064125704	1.4003528275512296
9	82.84348638702396	3.436077008448138	11.67414638116959	2.046290223358311
10	53.22639976582519	20.63023703937925	16.090986930979483	10.052376263816079
11	47.401927559212886	16.673841139942926	22.68591930048103	13.238312000363159
12	42.43800081084593	18.35924231300844	26.658281664081247	12.544475212064384
13	19.997839831319002	41.45653286665341	26.039972512636204	12.505654789391379
14	13.407212145783209	42.54366123545995	31.005777668551858	13.04334895020498
15	11.662797668896232	20.55134392233411	53.990755104181176	13.79510330458848
16	13.379662168402367	15.044322591451053	51.62310065603384	19.95291458411274
17	14.1768113718793	15.227858662354679	32.19073976385287	38.40459020191316
18	19.539273588494126	20.120405923871274	38.603857936037095	21.73646255159751
19	27.549272978011675	21.86341159509673	28.809527909222826	21.77778751766877
20	30.20534125186471	20.443335488284216	28.171573745497692	21.179749514353382
21	20.817842993305042	26.526166999823115	31.29528723199429	21.360702774877552
22	21.32743930786943	22.461840933318097	27.824851018801294	28.385868740011176
23	17.863107919209693	28.907048567792508	27.501843187407154	25.728000325590646
24	18.95360176820764	21.878204054542692	42.077894430365085	17.090299746884583
25	16.07486393285319	23.20733392920595	39.80885637852417	20.908945759416692
26	16.68526811919748	31.69178775873107	30.521852923819615	21.10109119825183
27	16.87655262123947	31.669403402109136	31.676838765322717	19.777205211328678
28	13.987327010404812	25.824894848310763	42.32099167395854	17.866786467325884
29	13.847307381045928	21.807920305429068	42.24162895502623	22.103143358498777
30	18.094543382605007	27.454961265671006	35.03347478785735	19.41702056386664
31	28.430793987217434	24.627410036018464	27.259763414569242	19.682032562194856
32	29.386277293653016	24.113822105413103	28.588580221307712	17.911320379626165
33	27.16192968807477	25.359597645103072	27.18572285035823	20.292749816463928
34	18.419820956453815	26.114326144771997	30.55065517289959	24.915197725874595
35	19.098317416438256	24.181679578109662	33.38712883840842	23.332874167043652
36	30.882507173168825	22.423177044607485	27.657985814892328	19.036329967331362
37	19.779709754726934	30.842904080683866	29.770489904342096	19.606896260247105
38	20.330631035362586	31.779838112576098	25.534837415999963	22.354693436061353
39	20.279209628717094	29.855879180826467	28.18276592380866	21.68214526664778
40	22.843470733627722	24.88592587490745	27.17578294374639	25.09482044771844
41	17.6772238388702	22.76935190243551	28.455448086294044	31.097976172400244
42	21.955531831963924	24.794431773889823	24.500382725538046	28.74965366860821
43	21.912876327212334	25.84657480210194	26.51959257340269	25.72095629728304
44	19.916050835969624	29.40365256347843	28.6843790062911	21.99591759426084
45	16.999353514735326	35.74476511296273	24.19960271680345	23.05627865549849
46	22.13171080663516	30.457282664333267	28.10050732657211	19.31049920245946
47	22.033642279197103	26.88713431709711	27.903744135846438	23.17547926785935
48	22.442822056887575	22.668465763674416	31.029492563854117	23.859219615583896
49	19.571284783803115	22.4972176088185	34.46369116475356	23.467806442624823
50	19.085794699446964	31.327768029190455	28.261580773872602	21.32485649748998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	587.0
1	979.0
2	1371.0
3	11695.0
4	22019.0
5	16282.5
6	10546.0
7	11247.5
8	11949.0
9	12650.0
10	13351.0
11	13155.5
12	12960.0
13	11997.5
14	11035.0
15	9938.0
16	8841.0
17	8239.0
18	7637.0
19	6755.0
20	5873.0
21	5464.5
22	5056.0
23	5175.0
24	5294.0
25	5304.0
26	5314.0
27	6772.0
28	8230.0
29	9926.5
30	11623.0
31	13073.0
32	14523.0
33	18158.0
34	21793.0
35	24112.5
36	26432.0
37	30549.5
38	34667.0
39	44623.5
40	54580.0
41	75826.5
42	97073.0
43	111222.0
44	125371.0
45	129107.0
46	132843.0
47	123088.5
48	113334.0
49	108759.0
50	104184.0
51	93461.5
52	82739.0
53	76676.5
54	70614.0
55	62703.5
56	54793.0
57	50797.5
58	46802.0
59	44222.0
60	41642.0
61	37513.0
62	33384.0
63	30325.0
64	27266.0
65	23590.5
66	19915.0
67	16343.5
68	12772.0
69	10727.5
70	8683.0
71	7105.0
72	5527.0
73	4609.0
74	3691.0
75	2683.5
76	1676.0
77	1228.5
78	781.0
79	646.5
80	512.0
81	358.0
82	204.0
83	153.0
84	102.0
85	70.5
86	39.0
87	34.0
88	29.0
89	21.0
90	13.0
91	9.0
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1277678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.821438452044344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18818043811966	16.092665215876593
2	7.1523585840714885	2.8354007092224807
3	2.335787441007624	1.3889600099697232
4	1.2317427184099954	0.9765964992687004
5	0.767690020738516	0.760836024815857
6	0.5379723999612794	0.639803208883865
7	0.4219076311055665	0.5853971299684771
8	0.32158786677544016	0.5099467286570899
9	0.28604203503237	0.5102788132882471
>10	3.0914088858754627	14.404131382915741
>50	1.3586193247821727	19.578083492237162
>100	1.299514192086845	39.68542203557349
>500	0.0063897440299291695	0.7082328777920693
>1k	3.993590018705731E-4	0.3252630931985708
>5k	0.0	0.0
>10k+	3.993590018705731E-4	0.9989827783319453
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12620	0.9877293026881577	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4109	0.3215990257326181	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06018730853939725	0.0
2	0.0	0.0	0.0	0.23002665773379521	0.0
3	0.0	0.0	0.0	0.3227730304505517	0.0
4	0.0	0.0	0.0	0.47625458057507447	0.0
5	0.0	0.0	0.0	0.7896355732821572	0.0
6	0.0	0.0	0.0	1.0783624669126337	0.0
7	0.0	0.0	0.0	1.2485148840318139	0.0
8	0.0	0.0	0.0	1.6518246381326125	0.0
9	0.0	0.0	0.0	1.7944270778709503	0.0
10	0.0	0.0	0.0	2.032123899761912	0.0
11	0.0	0.0	0.0	2.2899353358201364	0.0
12	0.0	0.0	0.0	2.503447660521665	0.0
13	0.0	0.0	0.0	2.5921241502162515	0.0
14	0.0	0.0	0.0	2.624839748356002	0.0
15	0.0	0.0	0.0	2.679861436136491	0.0
16	0.0	0.0	0.0	2.810489027751906	0.0
17	0.0	0.0	0.0	2.962092170327735	0.0
18	0.0	0.0	0.0	3.2348525997943143	0.0
19	0.0	0.0	0.0	3.321337614015425	0.0
20	0.0	0.0	0.0	3.4297373829712963	0.0
21	0.0	0.0	0.0	3.5642000566652943	0.0
22	0.0	0.0	0.0	3.6924013718636464	0.0
23	0.0	0.0	0.0	3.8591100418102213	0.0
24	0.0	0.0	0.0	3.9708752909575025	0.0
25	7.826698119557509E-5	0.0	0.0	4.065891406128931	0.0
26	7.826698119557509E-5	0.0	0.0	4.162238059980684	0.0
27	7.826698119557509E-5	0.0	0.0	4.250758015712879	0.0
28	7.826698119557509E-5	0.0	0.0	4.347730805414197	0.0
29	7.826698119557509E-5	0.0	0.0	4.4495561479496395	0.0
30	7.826698119557509E-5	0.0	0.0	4.587932170703417	0.0
31	7.826698119557509E-5	0.0	0.0	4.718011893450463	0.0
32	7.826698119557509E-5	0.0	0.0	4.825863793537965	0.0
33	7.826698119557509E-5	0.0	0.0	4.933637426644272	0.0
34	7.826698119557509E-5	0.0	0.0	5.052994572967524	0.0
35	7.826698119557509E-5	0.0	0.0	5.216885631591058	0.0
36	7.826698119557509E-5	0.0	0.0	5.336947180745071	0.0
37	7.826698119557509E-5	0.0	0.0	5.455599924237562	0.0
38	7.826698119557509E-5	0.0	0.0	5.570104517726689	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	40	8.314601E-9	44.000004	1
ACATTCG	20	7.8568107E-4	44.000004	14
ACTACGC	20	7.8568107E-4	44.000004	10
CGTACGT	20	7.8568107E-4	44.000004	35
TCGTAAT	20	7.8568107E-4	44.000004	35
GACGATT	40	8.314601E-9	44.000004	35
ATCATAA	20	7.8568107E-4	44.000004	21
TGTCGTG	20	7.8568107E-4	44.000004	13
ATCGCTA	20	7.8568107E-4	44.000004	10
GTACGCC	20	7.8568107E-4	44.000004	22
AACACGT	20	7.8568107E-4	44.000004	41
ATCTCGT	45	4.802132E-10	44.0	19
CTCACGT	30	2.5279387E-6	44.0	44
ACCGGTT	25	4.4431188E-5	44.0	18
GGTGTTA	30	2.5279387E-6	44.0	8
GTCGATG	25	4.4431188E-5	44.0	1
GATCGAT	30	2.5279387E-6	44.0	9
CTATCCG	30	2.5279387E-6	44.0	44
CGGCATG	25	4.4431188E-5	44.0	19
GCTATAA	30	2.5279387E-6	44.0	36
>>END_MODULE