FastQCFastQC Report
Thu 26 May 2016
SRR1545940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545940_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1707215
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177181.0378306188734283No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37840.22164753707060916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA302.5284426E-644.035
GTACCGA351.4466423E-744.044
CGAACTA207.8575575E-444.039
CGTATAA207.8575575E-444.031
GCGAATC207.8575575E-444.012
CTATCGG207.8575575E-444.02
CTATCGA207.8575575E-444.031
CGGTTGC207.8575575E-444.020
CGTTGCA302.5284426E-644.026
TAGCGAA302.5284426E-644.043
GTTAGCG1400.044.01
AAGCGAT502.7284841E-1144.028
CGTTACG207.8575575E-444.024
AAGCCCG207.8575575E-444.038
CTACGCG302.5284426E-644.01
CATACGT207.8575575E-444.040
ACTAACG408.3164196E-944.01
CGTACCA207.8575575E-444.020
CGGTCCC254.4437515E-544.012
ATACGCG351.4466423E-744.01
Produced by FastQC (version 0.11.5)