##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545940_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1707215
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.604048113447927	31.0	31.0	33.0	30.0	34.0
2	32.01114095178404	31.0	31.0	34.0	30.0	34.0
3	32.007381612743565	33.0	31.0	34.0	30.0	34.0
4	35.76910172415308	37.0	35.0	37.0	35.0	37.0
5	35.71972832947227	37.0	35.0	37.0	33.0	37.0
6	35.78191616170195	37.0	35.0	37.0	33.0	37.0
7	36.07116561182979	37.0	35.0	37.0	35.0	37.0
8	36.0789045316495	37.0	35.0	37.0	35.0	37.0
9	37.89900979079964	39.0	38.0	39.0	35.0	39.0
10	37.41993363460372	39.0	37.0	39.0	35.0	39.0
11	37.0711099656458	39.0	37.0	39.0	33.0	39.0
12	36.247080771900436	38.0	35.0	39.0	33.0	39.0
13	35.916189232170524	38.0	35.0	39.0	32.0	39.0
14	36.927139815430394	39.0	35.0	41.0	32.0	41.0
15	37.14418277721318	39.0	35.0	41.0	32.0	41.0
16	37.337540380092726	39.0	35.0	41.0	33.0	41.0
17	37.280665294060796	39.0	35.0	41.0	33.0	41.0
18	37.13400069704167	38.0	35.0	40.0	33.0	41.0
19	36.92688149998682	37.0	35.0	40.0	32.0	41.0
20	36.66354501336973	37.0	35.0	40.0	32.0	41.0
21	36.522381188075315	37.0	35.0	40.0	32.0	41.0
22	36.405075517729166	37.0	35.0	40.0	32.0	41.0
23	36.38195423540679	37.0	35.0	40.0	32.0	41.0
24	36.39400602736035	37.0	35.0	40.0	32.0	41.0
25	36.355037297587	37.0	35.0	40.0	32.0	41.0
26	36.12674502039872	36.0	35.0	40.0	31.0	41.0
27	36.11196890842688	36.0	35.0	40.0	31.0	41.0
28	36.12354682919258	36.0	35.0	40.0	31.0	41.0
29	36.118140363105994	36.0	35.0	40.0	31.0	41.0
30	35.98368922484865	36.0	35.0	40.0	31.0	41.0
31	35.70778490114016	36.0	35.0	40.0	30.0	41.0
32	35.36268835501094	36.0	34.0	40.0	29.0	41.0
33	34.987547555521715	36.0	34.0	40.0	27.0	41.0
34	34.649918141534606	36.0	34.0	40.0	24.0	41.0
35	34.34413533151946	36.0	34.0	40.0	23.0	41.0
36	34.142021362277156	36.0	34.0	40.0	21.0	41.0
37	34.03152737060066	36.0	33.0	40.0	21.0	41.0
38	33.99416007942761	36.0	33.0	40.0	21.0	41.0
39	33.92569067164944	36.0	33.0	40.0	21.0	41.0
40	33.75300123300229	36.0	33.0	40.0	18.0	41.0
41	33.73421156679153	36.0	33.0	40.0	18.0	41.0
42	33.69762859393808	36.0	33.0	40.0	18.0	41.0
43	33.59289544667778	35.0	33.0	40.0	18.0	41.0
44	33.460420040826726	35.0	33.0	40.0	18.0	41.0
45	33.35709093465088	35.0	33.0	40.0	18.0	41.0
46	33.33895730766189	35.0	33.0	40.0	18.0	41.0
47	33.3151530416497	35.0	33.0	40.0	18.0	41.0
48	33.232923211194844	35.0	33.0	40.0	18.0	41.0
49	33.19464625135088	35.0	33.0	40.0	18.0	41.0
50	33.00909844395697	35.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	2.0
14	6.0
15	24.0
16	89.0
17	281.0
18	680.0
19	1428.0
20	2567.0
21	4201.0
22	6637.0
23	10494.0
24	16293.0
25	26284.0
26	38850.0
27	46857.0
28	44736.0
29	41834.0
30	43344.0
31	48878.0
32	60211.0
33	79288.0
34	148985.0
35	273600.0
36	98313.0
37	121409.0
38	200394.0
39	391526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.087130209141787	22.363498446299968	28.981996995106062	18.56737434945218
2	35.503261159256446	23.70509865482672	28.702828876269244	12.088811309647584
3	26.194884651318084	23.310069323430266	38.18183415679923	12.313211868452422
4	24.02034893086108	25.288496176521413	36.77041263109801	13.920742261519493
5	21.0029199602862	30.892711228521307	35.34446452262896	12.759904288563536
6	18.783222968401752	38.4725415369476	32.59255571208078	10.15167978256986
7	83.96798294298023	2.9445617570136156	12.011023801923015	1.0764314980831353
8	86.018749835258	1.8255462844457202	11.248085331958775	0.9076185483374971
9	82.22731173285146	4.035344113073045	12.25364116411817	1.4837029899573282
10	47.57725301148362	27.97966278412502	16.731167427652636	7.7119167767387236
11	41.985924444197124	17.775499863813288	29.158893285262838	11.079682406726745
12	38.13239691544416	20.0820634776522	29.77656592754867	12.008973679354973
13	19.089394130206212	38.633271146282105	29.809777913150953	12.467556810360733
14	13.993375175358699	39.77325644397454	32.79089042680623	13.442477953860527
15	12.521152871782407	22.19076097620979	52.34185500947449	12.946231142533307
16	14.758070893238404	17.9438442141148	50.195200955942866	17.10288393670393
17	15.527511180489862	17.363776677219917	34.193232838277545	32.91547930401267
18	20.406627167638522	21.48405443954042	38.92456427573563	19.18475411708543
19	25.758559993908207	22.499743734678994	32.549385988290865	19.192310283121927
20	28.67992607843769	21.331993919922212	31.193962096162466	18.794117905477634
21	21.19223413571226	25.787437434652343	33.42513977442794	19.59518865520746
22	21.177356103361323	21.743424231863003	32.400781389573076	24.678438275202595
23	17.420535784889427	27.994657966336987	31.205501357474013	23.379304891299572
24	17.775734163535347	22.304279191548808	43.978292130750965	15.941694514164883
25	15.341945800616793	23.843569790565336	41.203890546884836	19.610593861933033
26	16.04871091221668	31.777192679305184	33.50163863368117	18.672457774796964
27	15.968170382757885	32.60819521852842	34.21191824111199	17.211716157601707
28	13.323805144636147	26.492445298336765	44.224482563707554	15.95926699331953
29	14.22562477485261	22.152160097000085	43.06920920915058	20.55300591899673
30	16.765199462282137	27.834221231655064	37.50224781295854	17.898331493104266
31	26.069944324528542	24.624197889545254	31.327688662529322	17.978169123396878
32	27.189486971471077	24.347899942303695	32.2023295249866	16.260283561238627
33	26.11153252519454	26.258028426413777	29.621928110987778	18.0085109374039
34	18.82065234900115	26.60795506131331	32.27103791848127	22.30035467120427
35	19.200100748880487	25.64920059863579	34.358882741775346	20.791815910708376
36	28.6311917362488	24.33764932946348	29.619877988419734	17.411280945867976
37	19.730906769211845	30.52532926432816	31.568080177364887	18.175683789095103
38	19.89708384708429	31.35041573556933	27.712971125488	21.03952929185838
39	20.242851661917214	30.862193689722734	29.4798253295572	19.415129318802844
40	22.72619441605187	26.458413263707264	28.348860571164145	22.46653174907671
41	17.219038023916145	25.37079395389567	30.21189481113978	27.198273211048402
42	22.122111157645637	25.009093757962532	27.088035191818253	25.780759892573577
43	22.137750664093275	26.0588150877306	28.21694982764327	23.586484420532855
44	20.04451694719177	29.618472190087363	30.036345744384864	20.300665118336003
45	17.48608113213626	36.233456243062534	25.26096595917913	21.019496665622082
46	21.387757253772957	31.585066907214383	28.71354808855358	18.31362775045908
47	21.350269298243045	27.230723722553986	29.482871225944006	21.936135753258963
48	23.223964175572497	23.64060765632917	32.231499840383314	20.903928327715022
49	20.93263004366761	23.355757769232348	33.85326394156565	21.858348245534394
50	18.766763412926903	32.404295885404004	29.214539469252554	19.614401232416537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	502.0
1	1167.5
2	1833.0
3	17285.0
4	32737.0
5	24632.5
6	16528.0
7	17432.5
8	18337.0
9	19066.5
10	19796.0
11	19512.0
12	19228.0
13	18061.0
14	16894.0
15	15498.5
16	14103.0
17	12719.0
18	11335.0
19	10540.5
20	9746.0
21	9641.5
22	9537.0
23	9540.5
24	9544.0
25	10507.0
26	11470.0
27	12548.0
28	13626.0
29	18846.5
30	24067.0
31	27800.5
32	31534.0
33	37207.5
34	42881.0
35	45841.0
36	48801.0
37	54209.5
38	59618.0
39	72582.5
40	85547.0
41	111084.0
42	136621.0
43	155802.0
44	174983.0
45	176721.0
46	178459.0
47	167085.0
48	155711.0
49	144759.5
50	133808.0
51	123010.5
52	112213.0
53	100071.0
54	87929.0
55	76807.5
56	65686.0
57	57834.5
58	49983.0
59	43779.0
60	37575.0
61	32309.0
62	27043.0
63	23023.5
64	19004.0
65	15843.5
66	12683.0
67	9947.5
68	7212.0
69	5835.5
70	4459.0
71	3560.5
72	2662.0
73	2117.5
74	1573.0
75	1298.0
76	1023.0
77	756.0
78	489.0
79	348.5
80	208.0
81	162.5
82	117.0
83	94.0
84	71.0
85	46.0
86	21.0
87	14.5
88	8.0
89	7.0
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1707215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.01116744136689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27188179842702	15.26062185703507
2	6.961130512878612	2.646784355230869
3	2.3544851676297323	1.3428453528007085
4	1.2678277537971212	0.9641154285699658
5	0.8498951421951697	0.8078749427938345
6	0.6200575542792378	0.7072810792612216
7	0.49442144870975263	0.6579670263617009
8	0.40172186413866257	0.6109761299198524
9	0.32716150256935933	0.5597749895143748
>10	3.851137380490013	16.978134855109413
>50	1.2706588682600692	17.4994669685737
>100	1.325877352922913	40.32265196470679
>500	0.003119711418640382	0.3662352742170896
>1k	3.1197114186403823E-4	0.2244266036659866
>5k	0.0	0.0
>10k+	3.1197114186403823E-4	1.050843172239416
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17718	1.0378306188734283	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3784	0.22164753707060916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01675243012742976	0.0
2	0.0	0.0	0.0	0.10215467881901225	0.0
3	0.0	0.0	0.0	0.15534071572707597	0.0
4	0.0	0.0	0.0	0.2292037031071072	0.0
5	0.0	0.0	0.0	0.4019411731972833	0.0
6	0.0	0.0	0.0	0.5423452816429096	0.0
7	0.0	0.0	0.0	0.6354794211625366	0.0
8	0.0	0.0	0.0	0.8437718740756144	0.0
9	0.0	0.0	0.0	0.9369060135952414	0.0
10	0.0	0.0	0.0	1.1389895238736774	0.0
11	0.0	0.0	0.0	1.3313495956865422	0.0
12	0.0	0.0	0.0	1.4975266735589834	0.0
13	0.0	0.0	0.0	1.5630720208058153	0.0
14	0.0	0.0	0.0	1.5857990938458248	0.0
15	0.0	0.0	0.0	1.6263915206930586	0.0
16	0.0	0.0	0.0	1.722220107016398	0.0
17	0.0	0.0	0.0	1.8437045129055216	0.0
18	0.0	0.0	0.0	2.0219480264641536	0.0
19	0.0	0.0	0.0	2.0975682617596494	0.0
20	0.0	0.0	0.0	2.186192131629584	0.0
21	0.0	0.0	0.0	2.2909241073912776	0.0
22	0.0	0.0	0.0	2.397999080373591	0.0
23	0.0	0.0	0.0	2.52498952973117	0.0
24	0.0	0.0	0.0	2.6127347756433723	0.0
25	0.0	0.0	0.0	2.68794498642526	0.0
26	0.0	0.0	0.0	2.765732494149829	0.0
27	0.0	0.0	0.0	2.8516033422855354	0.0
28	0.0	0.0	0.0	2.943390258403306	0.0
29	0.0	0.0	0.0	3.029964005705198	0.0
30	0.0	0.0	0.0	3.156778730271231	0.0
31	0.0	0.0	0.0	3.2739285913022087	0.0
32	5.8574930515488676E-5	0.0	0.0	3.3706358015832802	0.0
33	5.8574930515488676E-5	0.0	0.0	3.4673430118643522	0.0
34	5.8574930515488676E-5	0.0	0.0	3.573422211027902	0.0
35	5.8574930515488676E-5	0.0	0.0	3.7157592921805396	0.0
36	5.8574930515488676E-5	0.0	0.0	3.831210480226568	0.0
37	5.8574930515488676E-5	0.0	0.0	3.9469545429251736	0.0
38	5.8574930515488676E-5	0.0	0.0	4.056079638475529	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	30	2.5284426E-6	44.0	35
GTACCGA	35	1.4466423E-7	44.0	44
CGAACTA	20	7.8575575E-4	44.0	39
CGTATAA	20	7.8575575E-4	44.0	31
GCGAATC	20	7.8575575E-4	44.0	12
CTATCGG	20	7.8575575E-4	44.0	2
CTATCGA	20	7.8575575E-4	44.0	31
CGGTTGC	20	7.8575575E-4	44.0	20
CGTTGCA	30	2.5284426E-6	44.0	26
TAGCGAA	30	2.5284426E-6	44.0	43
GTTAGCG	140	0.0	44.0	1
AAGCGAT	50	2.7284841E-11	44.0	28
CGTTACG	20	7.8575575E-4	44.0	24
AAGCCCG	20	7.8575575E-4	44.0	38
CTACGCG	30	2.5284426E-6	44.0	1
CATACGT	20	7.8575575E-4	44.0	40
ACTAACG	40	8.3164196E-9	44.0	1
CGTACCA	20	7.8575575E-4	44.0	20
CGGTCCC	25	4.4437515E-5	44.0	12
ATACGCG	35	1.4466423E-7	44.0	1
>>END_MODULE