FastQCFastQC Report
Thu 26 May 2016
SRR1545939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545939_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences787240
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88801.1279914638483817No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10490.13325034297037752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG254.4415574E-544.01
AAGCGTA207.854968E-444.028
TTGCGGT207.854968E-444.017
CGTTTTT56700.043.185191
CGAGGGT1150.042.0869524
TATAGCG900.041.5555571
TCACGAC2150.040.93023325
CACGACG2100.040.85714326
CATACGA3300.040.66666818
ACATACG3350.040.05970417
TTAATCG1650.040.020
CGTTGAT1650.040.025
ACGTTTG501.3460522E-939.6000021
ACGTAAG501.3460522E-939.6000021
CGGGTAT950.039.3684236
TCGAAGG1350.039.111112
TATGGGC2650.039.0188674
CGACGGT2200.039.00000428
ATACGAA3450.038.89855219
CCGCTCG850.038.82353219
Produced by FastQC (version 0.11.5)