##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545939_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	787240
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55389207865454	31.0	31.0	33.0	30.0	34.0
2	31.96188709923276	31.0	31.0	34.0	30.0	34.0
3	32.04170646816727	33.0	31.0	34.0	30.0	34.0
4	35.81582490727097	37.0	35.0	37.0	35.0	37.0
5	35.760267516894466	37.0	35.0	37.0	35.0	37.0
6	35.81896372135562	37.0	35.0	37.0	35.0	37.0
7	36.02370941517199	37.0	35.0	37.0	35.0	37.0
8	36.00750597022509	37.0	35.0	37.0	35.0	37.0
9	37.84353945429602	39.0	38.0	39.0	35.0	39.0
10	37.27673263553681	39.0	37.0	39.0	34.0	39.0
11	37.04581195061227	39.0	37.0	39.0	33.0	39.0
12	36.53589248513795	39.0	35.0	39.0	33.0	39.0
13	36.320272089832834	39.0	35.0	39.0	32.0	39.0
14	37.35305497688125	40.0	35.0	41.0	32.0	41.0
15	37.480623443930696	40.0	35.0	41.0	33.0	41.0
16	37.61306209034094	40.0	35.0	41.0	33.0	41.0
17	37.55288857273513	39.0	35.0	41.0	33.0	41.0
18	37.42967964026218	39.0	35.0	41.0	33.0	41.0
19	37.28523703063869	38.0	35.0	41.0	33.0	41.0
20	37.0908719069153	38.0	35.0	41.0	32.0	41.0
21	36.977048930440525	38.0	35.0	41.0	32.0	41.0
22	36.898156851785984	38.0	35.0	41.0	32.0	41.0
23	36.83499822163508	38.0	35.0	41.0	32.0	41.0
24	36.86768710939485	38.0	35.0	41.0	32.0	41.0
25	36.81916442253951	38.0	35.0	41.0	32.0	41.0
26	36.60175041918602	38.0	35.0	41.0	32.0	41.0
27	36.59147528072761	38.0	35.0	41.0	31.0	41.0
28	36.57283039479701	38.0	35.0	41.0	31.0	41.0
29	36.48594329556425	38.0	35.0	41.0	31.0	41.0
30	36.35987373609065	38.0	35.0	40.0	31.0	41.0
31	36.14429271886591	38.0	35.0	40.0	30.0	41.0
32	35.92147502667547	38.0	35.0	40.0	30.0	41.0
33	35.65116609928357	38.0	35.0	41.0	29.0	41.0
34	35.37815786799451	38.0	35.0	40.0	27.0	41.0
35	35.1349677353793	38.0	34.0	40.0	25.0	41.0
36	34.95290000508104	38.0	34.0	40.0	24.0	41.0
37	34.87189167217113	38.0	34.0	40.0	24.0	41.0
38	34.77350617346679	38.0	34.0	40.0	23.0	41.0
39	34.652953356028654	37.0	34.0	40.0	23.0	41.0
40	34.50072658909608	37.0	34.0	40.0	23.0	41.0
41	34.43302169605203	37.0	34.0	40.0	23.0	41.0
42	34.4187312636553	37.0	34.0	40.0	23.0	41.0
43	34.31307352268686	37.0	34.0	40.0	23.0	41.0
44	34.16933717798892	37.0	33.0	40.0	22.0	41.0
45	34.06840099588435	36.0	33.0	40.0	22.0	41.0
46	34.02339820131091	36.0	33.0	40.0	22.0	41.0
47	33.97017173924089	36.0	33.0	40.0	22.0	41.0
48	33.86599766272039	36.0	33.0	40.0	22.0	41.0
49	33.814548295310196	36.0	33.0	40.0	22.0	41.0
50	33.67795843707129	35.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	0.0
13	1.0
14	9.0
15	17.0
16	45.0
17	154.0
18	275.0
19	549.0
20	1032.0
21	1723.0
22	2786.0
23	4174.0
24	6232.0
25	9662.0
26	14292.0
27	17594.0
28	18074.0
29	17602.0
30	18758.0
31	21515.0
32	27218.0
33	34899.0
34	61569.0
35	96292.0
36	54152.0
37	70935.0
38	112240.0
39	195435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.73118743966262	21.580966414308218	29.078552919058993	16.609293226970173
2	34.69818606778111	23.569559473603984	29.473222905340172	12.259031553274733
3	27.4955540876988	23.6697830394797	35.932244296529646	12.902418576291854
4	24.4855444337178	26.089629591992274	35.045729383669524	14.379096590620396
5	21.463721355622173	30.604390020832273	34.120471520756055	13.811417102789491
6	19.45239063055739	40.41651846958996	30.246430567552462	9.884660332300188
7	85.88130684416441	3.0610741324119712	9.879706315735989	1.1779127076876175
8	87.80333824500788	1.7657893399725626	9.396753213759464	1.0341192012600986
9	84.53508459935979	3.1663787409176365	10.462628931456736	1.8359077282658403
10	43.912656877191196	23.22620801788527	18.95724302626899	13.90389207865454
11	37.3450281997866	22.87282150297241	24.658553935267516	15.123596361973476
12	35.17783649204817	20.70575682129973	28.135765459072203	15.9806412275799
13	23.067806513896652	30.00101620852599	28.88433006452924	18.046847213048117
14	18.883821960266246	31.959377064173566	31.063716274579544	18.09308470098064
15	18.163838219602663	22.73106041359687	41.49192114221839	17.613180224582084
16	20.38920786545399	18.587851227071795	40.782099486814694	20.24084142065952
17	21.18858289721051	19.38151008586962	31.148696712565417	28.281210304354452
18	23.349042223464256	22.845891977033688	33.81141710278949	19.993648696712565
19	27.25166404146131	24.184746710024896	28.254788882678728	20.30880036583507
20	27.890859204308725	22.690666124688786	28.86121132056298	20.557263350439513
21	23.60982673644632	24.869671256541842	31.740384126822825	19.780117880189017
22	23.405568822722422	21.27966058635232	31.073370255576442	24.241400335348814
23	21.33974391545145	25.977211523804684	29.311645749707836	23.371398811036023
24	20.958665718205378	23.56587571769727	36.42662974442356	19.048828819673798
25	20.98762766119608	23.383847365479397	34.07270972003455	21.555815253289975
26	19.583989634673035	27.940018291753464	29.712285961079214	22.763706112494283
27	19.149814541944007	28.08546313703572	32.17125654184238	20.59346577917789
28	17.83687312636553	25.89540673746253	36.458513286926475	19.809206849245463
29	19.048701793608046	23.39210405975306	36.03729485290382	21.521899293735075
30	21.627712006503735	26.007062649255623	32.79647883745745	19.568746506783192
31	26.69922768152025	24.632513591789035	29.09443117727758	19.57382754941314
32	26.69236827396982	24.858874040953204	29.17166302525278	19.277094659824197
33	25.805853361109698	25.26421421675728	28.358188100198163	20.57174432193486
34	20.491717900513184	25.64580051826635	30.948503632945478	22.913977948274987
35	21.71675727859357	24.774147655098826	31.38051928255678	22.128575783750826
36	26.970936436156702	25.489050353132463	27.992734109039176	19.547279101671663
37	21.383059803871753	28.997383263045577	29.168614399674812	20.450942533407858
38	22.153853970834817	28.468573751333775	27.309333875311214	22.068238402520198
39	21.679157563131955	28.181621868807476	27.425181647274023	22.714038920786546
40	23.69620446115543	25.10606676490016	28.492581677760278	22.705147096184135
41	20.287841064986537	23.992683298612874	29.416061175753267	26.303414460647325
42	22.670214928103245	25.142142167572786	27.079162644174588	25.108480260149385
43	22.328514811239266	25.930592957674914	27.540267262842335	24.200624968243485
44	21.90691529901936	27.27656115034805	29.102687871551243	21.71383567908135
45	20.486001727554495	30.371551242314922	26.311036024592244	22.831411005538335
46	22.404222346425488	28.45815761394238	27.927569737310098	21.21005030232204
47	22.172907880697117	27.12438392358112	28.884965194857987	21.817743000863775
48	22.2714801077181	25.716045932625377	30.176820283522176	21.835653676134342
49	21.710787053503378	25.300162593364156	30.59994410853107	22.389106244601393
50	20.92614704537371	28.10362786443778	29.357375133377367	21.612849956811136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	274.0
1	790.5
2	1307.0
3	8454.5
4	15602.0
5	11065.0
6	6528.0
7	6448.0
8	6368.0
9	6341.5
10	6315.0
11	6179.5
12	6044.0
13	5558.5
14	5073.0
15	4649.5
16	4226.0
17	3869.5
18	3513.0
19	3287.0
20	3061.0
21	2890.5
22	2720.0
23	2990.0
24	3260.0
25	3753.5
26	4247.0
27	5121.0
28	5995.0
29	6639.5
30	7284.0
31	9788.0
32	12292.0
33	14796.5
34	17301.0
35	19131.5
36	20962.0
37	22440.5
38	23919.0
39	27830.0
40	31741.0
41	39867.0
42	47993.0
43	53288.0
44	58583.0
45	60110.5
46	61638.0
47	60681.5
48	59725.0
49	60865.0
50	62005.0
51	59454.5
52	56904.0
53	53239.5
54	49575.0
55	45735.5
56	41896.0
57	40442.0
58	38988.0
59	36641.0
60	34294.0
61	30301.0
62	26308.0
63	23587.0
64	20866.0
65	17463.0
66	14060.0
67	11941.5
68	9823.0
69	8046.5
70	6270.0
71	5337.0
72	4404.0
73	3574.0
74	2744.0
75	2134.0
76	1524.0
77	1189.5
78	855.0
79	602.0
80	349.0
81	277.5
82	206.0
83	157.0
84	108.0
85	78.5
86	49.0
87	37.0
88	25.0
89	15.5
90	6.0
91	4.5
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	787240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.510553937522516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04946560361559	17.659723128470933
2	7.194224177980386	3.5266883950603365
3	3.3885593874156044	2.4916640290724525
4	2.1602769294871984	2.11798336800726
5	1.5101521069907011	1.8507332336129427
6	1.1766989911892503	1.7304926455063472
7	1.0071606955675638	1.7280246586723027
8	0.8307219348538181	1.628916383305406
9	0.7034437608311328	1.5517616617678653
>10	9.149176571034277	50.74071642783665
>50	0.7872006563152326	12.093584147738955
>100	0.04187237533585813	1.6059265748845797
>500	0.0	0.0
>1k	5.234046916982266E-4	0.1345755693444579
>5k	5.234046916982266E-4	1.1392097767195293
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8880	1.1279914638483817	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1049	0.13325034297037752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020070118388293277	0.0
2	0.0	0.0	0.0	0.06554544992632488	0.0
3	0.0	0.0	0.0	0.0926020019307962	0.0
4	0.0	0.0	0.0	0.1286774046034246	0.0
5	0.0	0.0	0.0	0.21010111274833596	0.0
6	0.0	0.0	0.0	0.2955896549972054	0.0
7	0.0	0.0	0.0	0.3464000812966821	0.0
8	0.0	0.0	0.0	0.4702504954016564	0.0
9	0.0	0.0	0.0	0.5313500330267771	0.0
10	0.0	0.0	0.0	0.6590112291042122	0.0
11	0.0	0.0	0.0	0.8162694985010924	0.0
12	0.0	0.0	0.0	0.9208119506122656	0.0
13	0.0	0.0	0.0	0.9641278390325695	0.0
14	0.0	0.0	0.0	0.9850871398811036	0.0
15	0.0	0.0	0.0	1.014176108937554	0.0
16	0.0	0.0	0.0	1.077562115746151	0.0
17	0.0	0.0	0.0	1.1527615466693766	0.0
18	0.0	0.0	0.0	1.2546364513998272	0.0
19	1.2702606574869162E-4	0.0	0.0	1.3012550175295972	0.0
20	1.2702606574869162E-4	0.0	0.0	1.3570194603932726	0.0
21	1.2702606574869162E-4	0.0	0.0	1.4282810832782886	0.0
22	1.2702606574869162E-4	0.0	0.0	1.5062750876479853	0.0
23	1.2702606574869162E-4	0.0	0.0	1.6000203241705198	0.0
24	1.2702606574869162E-4	0.0	0.0	1.6679792693460698	0.0
25	1.2702606574869162E-4	0.0	0.0	1.7220923733550124	0.0
26	1.2702606574869162E-4	0.0	0.0	1.7830648849143844	0.0
27	1.2702606574869162E-4	0.0	0.0	1.8554697423911386	0.0
28	1.2702606574869162E-4	0.0	0.0	1.9244448960926783	0.0
29	1.2702606574869162E-4	0.0	0.0	2.003201056856867	0.0
30	1.2702606574869162E-4	0.0	0.0	2.0925003810781972	0.0
31	1.2702606574869162E-4	0.0	0.0	2.1671917077384277	0.0
32	1.2702606574869162E-4	0.0	0.0	2.2416289822671613	0.0
33	1.2702606574869162E-4	0.0	0.0	2.317209491387633	0.0
34	1.2702606574869162E-4	0.0	0.0	2.40193587724201	0.0
35	1.2702606574869162E-4	1.2702606574869162E-4	0.0	2.5038107819724607	0.0
36	1.2702606574869162E-4	1.2702606574869162E-4	0.0	2.590188506681571	0.0
37	1.2702606574869162E-4	1.2702606574869162E-4	0.0	2.6802499872973935	0.0
38	1.2702606574869162E-4	1.2702606574869162E-4	0.0	2.7872059346577918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	25	4.4415574E-5	44.0	1
AAGCGTA	20	7.854968E-4	44.0	28
TTGCGGT	20	7.854968E-4	44.0	17
CGTTTTT	5670	0.0	43.18519	1
CGAGGGT	115	0.0	42.086952	4
TATAGCG	90	0.0	41.555557	1
TCACGAC	215	0.0	40.930233	25
CACGACG	210	0.0	40.857143	26
CATACGA	330	0.0	40.666668	18
ACATACG	335	0.0	40.059704	17
TTAATCG	165	0.0	40.0	20
CGTTGAT	165	0.0	40.0	25
ACGTTTG	50	1.3460522E-9	39.600002	1
ACGTAAG	50	1.3460522E-9	39.600002	1
CGGGTAT	95	0.0	39.368423	6
TCGAAGG	135	0.0	39.11111	2
TATGGGC	265	0.0	39.018867	4
CGACGGT	220	0.0	39.000004	28
ATACGAA	345	0.0	38.898552	19
CCGCTCG	85	0.0	38.823532	19
>>END_MODULE