FastQCFastQC Report
Thu 26 May 2016
SRR1545938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545938_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1586939
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119980.7560467037485373No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32900.20731735750397465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG650.044.0000041
TCGCCTA207.8573887E-444.044
CGCATAA454.802132E-1044.012
TGCGGTC254.4436085E-544.042
TAACGCG502.7284841E-1144.01
TGCGGAT207.8573887E-444.04
CGTTCCA207.8573887E-444.021
ACCTCGC254.4436085E-544.040
CGCCTAG207.8573887E-444.041
CTACCGC207.8573887E-444.039
CCGCGAT207.8573887E-444.019
TGTCGCG302.5283298E-644.02
ACGCGTG254.4436085E-544.01
CGTTTTT118500.043.368781
CGGGTAT1100.042.0000046
CGACGGT800.041.2528
CGACACT700.040.85714334
TAGACGG3200.040.56252
TCGATAG603.6379788E-1240.3333321
CAATCGA501.3478711E-939.60000216
Produced by FastQC (version 0.11.5)