##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545938_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1586939
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.516017313835	31.0	31.0	33.0	30.0	34.0
2	31.921896808888054	31.0	31.0	34.0	30.0	34.0
3	31.960848526628936	33.0	31.0	34.0	30.0	34.0
4	35.72180153112375	37.0	35.0	37.0	33.0	37.0
5	35.65340570746576	37.0	35.0	37.0	33.0	37.0
6	35.71252518212735	37.0	35.0	37.0	33.0	37.0
7	36.019155745747	37.0	35.0	37.0	35.0	37.0
8	36.023761467832095	37.0	35.0	37.0	35.0	37.0
9	37.83551919765032	39.0	38.0	39.0	35.0	39.0
10	37.343195926245436	39.0	37.0	39.0	34.0	39.0
11	36.96676557826104	39.0	37.0	39.0	33.0	39.0
12	36.213724030980394	38.0	35.0	39.0	32.0	39.0
13	35.88714626082036	38.0	35.0	39.0	32.0	39.0
14	36.89729850989862	39.0	35.0	41.0	32.0	41.0
15	37.12441246954042	39.0	35.0	41.0	32.0	41.0
16	37.29660623376198	39.0	35.0	41.0	33.0	41.0
17	37.21885592325855	39.0	35.0	41.0	33.0	41.0
18	37.11150460099601	38.0	35.0	41.0	32.0	41.0
19	36.97365305156657	38.0	35.0	41.0	32.0	41.0
20	36.76603007424986	38.0	35.0	40.0	32.0	41.0
21	36.600396738627005	38.0	35.0	40.0	32.0	41.0
22	36.50219951743577	38.0	35.0	40.0	32.0	41.0
23	36.44950499042496	37.0	35.0	40.0	32.0	41.0
24	36.47291672836826	37.0	35.0	40.0	32.0	41.0
25	36.44566363294367	37.0	35.0	40.0	32.0	41.0
26	36.209215351062646	37.0	35.0	40.0	31.0	41.0
27	36.183862769772496	37.0	35.0	40.0	31.0	41.0
28	36.20044059664549	37.0	35.0	40.0	31.0	41.0
29	36.19119953570994	37.0	35.0	40.0	31.0	41.0
30	36.10413695800532	37.0	35.0	40.0	31.0	41.0
31	35.85860641146257	37.0	35.0	40.0	30.0	41.0
32	35.584188176105066	37.0	35.0	40.0	30.0	41.0
33	35.28745276283461	37.0	34.0	40.0	28.0	41.0
34	35.039830138398514	37.0	34.0	40.0	26.0	41.0
35	34.76962126458547	37.0	34.0	40.0	24.0	41.0
36	34.58607797779247	37.0	34.0	40.0	23.0	41.0
37	34.493468873094685	37.0	34.0	40.0	23.0	41.0
38	34.45384290133395	37.0	34.0	40.0	23.0	41.0
39	34.382524470064695	36.0	34.0	40.0	23.0	41.0
40	34.247569692344825	36.0	34.0	40.0	23.0	41.0
41	34.241498255446494	36.0	34.0	40.0	23.0	41.0
42	34.21491122217048	36.0	34.0	40.0	22.0	41.0
43	34.11268360031482	36.0	33.0	40.0	22.0	41.0
44	33.99505210975343	36.0	33.0	40.0	22.0	41.0
45	33.892149603733984	36.0	33.0	40.0	21.0	41.0
46	33.87397058109984	36.0	33.0	40.0	22.0	41.0
47	33.81926526476443	35.0	33.0	40.0	21.0	41.0
48	33.733168697725624	35.0	33.0	40.0	20.0	41.0
49	33.699096814685376	36.0	33.0	40.0	20.0	41.0
50	33.52169049976086	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	9.0
15	24.0
16	88.0
17	250.0
18	591.0
19	1276.0
20	2331.0
21	4083.0
22	6052.0
23	9327.0
24	14209.0
25	21649.0
26	31949.0
27	37426.0
28	38287.0
29	36987.0
30	39442.0
31	45919.0
32	56700.0
33	74244.0
34	134930.0
35	242635.0
36	92873.0
37	118845.0
38	193610.0
39	383200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.898894664508216	22.729796167338506	29.049194707547045	16.322114460606237
2	32.96238859842754	24.852183984387555	30.00921900589752	12.176208411287389
3	27.16084235121829	24.1166169588119	36.00737016356646	12.715170526403346
4	23.62245807809878	27.1792425543767	35.638988014032044	13.559311353492479
5	22.067829954396483	31.393330178412654	33.65025372745896	12.8885861397319
6	18.752768694952987	40.26714322352655	30.463174702997403	10.516913378523055
7	85.97060126444683	3.3888511152602585	9.633136497370094	1.0074111229228093
8	88.26432521980996	1.6899830428264726	9.17502185024125	0.8706698871223153
9	84.56619945694194	3.9824467103020345	10.053820594238342	1.3975332385176746
10	46.35691731062126	32.16544555272761	14.27931382365674	7.198323312994387
11	40.33179599215849	18.861846611621495	29.107546036741173	11.698811359478846
12	37.398917034618215	21.332830058370234	28.74760781605342	12.520645090958126
13	19.72810549113734	38.67810924049381	28.81194551271347	12.781839755655383
14	14.20029377310659	40.49273475540018	31.45243768033932	13.854533791153914
15	13.46668019375666	23.04505718241218	49.96411330240167	13.524149321429494
16	15.464551567514567	19.761251062580225	47.76711644240894	17.00708092749627
17	16.117128635694254	19.07880517146532	32.90485645636033	31.899209736480106
18	20.42315426112787	22.705157539136664	37.43603251290693	19.435655686828543
19	25.632995345126687	23.219733083628295	30.856384523916798	20.29088704732822
20	28.552515251058804	22.905039198104024	29.667744002762554	18.874701548074626
21	21.436677780305356	26.380094004873534	33.07928029999893	19.103947914822182
22	22.044451614082206	23.251177266422967	30.252265525013875	24.452105594480948
23	17.34338875029223	28.646469713076556	30.55460858924004	23.455532947391173
24	18.30347606303708	23.208768579006502	42.30042868692496	16.187326671031464
25	15.60368735030143	25.434500002835648	38.91995848611698	20.041854160745938
26	15.989650515867341	33.44356651389877	31.70991449576827	18.85686847446562
27	16.110323081101416	32.45033362971104	33.29989369471669	18.139449594470864
28	13.94161968418446	27.54113422129017	41.97855116044158	16.538694934083793
29	14.591865219772151	23.227483854136803	41.64961602178786	20.531034904303187
30	17.630167259106997	28.517227190207063	35.37155492429135	18.481050626394588
31	26.45615237888791	25.867786978579517	29.113595418601474	18.562465223931103
32	26.972177254450237	26.356715664559253	29.901086305144688	16.770020775845826
33	25.376715803190926	27.288446499833956	29.431818110210916	17.903019586764206
34	19.36671793937889	27.01754761840247	31.291435902703252	22.32429853951538
35	19.090588863214027	26.004906300746278	33.49158348241489	21.41292135362481
36	27.808252239058966	24.528037939706568	29.12569418232207	18.5380156389124
37	19.515683967688737	30.464245947701833	31.557797747739517	18.462272336869912
38	19.550026812624807	31.193007418684648	27.708689495941556	21.548276272748986
39	19.748963255676493	30.430911333075816	30.278353484286413	19.541771926961278
40	22.8858198078187	26.832978457268997	27.098206043206453	23.18299569170586
41	17.261533051995066	25.20260703152421	29.647516382167176	27.88834353431354
42	21.963100030940065	25.86759793539638	26.480286891934725	25.689015141728827
43	22.047161233040462	26.839405925495562	27.552854898644497	23.560577942819478
44	20.028621137926535	30.327504711901337	29.172576891739382	20.471297258432745
45	17.212066752408255	35.894196311263386	25.174439597237196	21.719297339091167
46	21.366416730573764	31.72541603678528	27.86767481295752	19.040492419683428
47	20.959784843651835	27.199029074211424	29.222232234509327	22.618953847627413
48	22.700053373192038	23.716286511327784	32.25416982001199	21.329490295468194
49	20.455921746204485	23.798142209624945	33.15407838612574	22.591857658044827
50	19.121213858881784	31.760326011270756	28.364921398995175	20.753538730852288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	301.0
1	794.0
2	1287.0
3	12002.0
4	22717.0
5	17430.5
6	12144.0
7	12498.0
8	12852.0
9	13522.0
10	14192.0
11	14190.5
12	14189.0
13	13521.5
14	12854.0
15	11738.0
16	10622.0
17	9679.5
18	8737.0
19	8438.0
20	8139.0
21	7772.0
22	7405.0
23	7680.0
24	7955.0
25	9275.0
26	10595.0
27	12954.5
28	15314.0
29	18989.5
30	22665.0
31	26890.0
32	31115.0
33	34617.5
34	38120.0
35	44055.0
36	49990.0
37	54348.5
38	58707.0
39	73333.5
40	87960.0
41	113529.5
42	139099.0
43	157538.5
44	175978.0
45	176157.5
46	176337.0
47	159235.5
48	142134.0
49	135373.0
50	128612.0
51	115275.0
52	101938.0
53	92642.0
54	83346.0
55	71829.0
56	60312.0
57	50470.5
58	40629.0
59	35301.5
60	29974.0
61	26808.5
62	23643.0
63	18745.5
64	13848.0
65	11497.5
66	9147.0
67	7461.0
68	5775.0
69	4513.5
70	3252.0
71	2687.0
72	2122.0
73	1739.5
74	1357.0
75	1075.5
76	794.0
77	621.0
78	448.0
79	321.0
80	194.0
81	134.5
82	75.0
83	57.5
84	40.0
85	27.0
86	14.0
87	10.0
88	6.0
89	5.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1586939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.6387644363872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94138768259252	15.503113169799526
2	6.842060329200591	2.6873922212944055
3	2.4704562621760147	1.4555012574981712
4	1.3332489892266144	1.0473345133789127
5	0.9245723089315011	0.9078728889756181
6	0.69798892253446	0.8224584017317182
7	0.5548295377473206	0.762731661591846
8	0.4473365649055793	0.7028109937570645
9	0.38993897968521	0.6892127789942712
>10	4.734704234123296	21.892303362806402
>50	1.5904657137054679	22.40417524070445
>100	1.0707309385018735	29.96555690895604
>500	0.0016282404782564193	0.1818191249933533
>1k	3.256480956512839E-4	0.2104062332846051
>5k	0.0	0.0
>10k+	3.256480956512839E-4	0.767311242233645
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11998	0.7560467037485373	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3290	0.20731735750397465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045685435924128145	0.0
2	0.0	0.0	0.0	0.14058511385755848	0.0
3	0.0	0.0	0.0	0.1925089748251193	0.0
4	0.0	0.0	0.0	0.26900844960014214	0.0
5	0.0	0.0	0.0	0.43542946515272485	0.0
6	0.0	0.0	0.0	0.5731159168688904	0.0
7	0.0	0.0	0.0	0.6677005228304301	0.0
8	0.0	0.0	0.0	0.901420911578832	0.0
9	0.0	0.0	0.0	0.9967616902729091	0.0
10	0.0	0.0	0.0	1.1719417066440487	0.0
11	0.0	0.0	0.0	1.3649547966241928	0.0
12	0.0	0.0	0.0	1.55853501615374	0.0
13	0.0	0.0	0.0	1.6285440083078178	0.0
14	0.0	0.0	0.0	1.6692513070760755	0.0
15	0.0	0.0	0.0	1.7090133899286615	0.0
16	0.0	0.0	0.0	1.8158227883995541	0.0
17	0.0	0.0	0.0	1.9599367083422867	0.0
18	0.0	0.0	0.0	2.154903244548152	0.0
19	0.0	0.0	0.0	2.2506221096084977	0.0
20	0.0	0.0	0.0	2.3447656148093907	0.0
21	0.0	0.0	0.0	2.4690300005230195	0.0
22	0.0	0.0	0.0	2.6057712363235135	0.0
23	0.0	0.0	0.0	2.7736415829467926	0.0
24	0.0	0.0	0.0	2.8958895080403217	0.0
25	0.0	0.0	0.0	2.988646696564896	0.0
26	0.0	0.0	0.0	3.0934396344156894	0.0
27	6.30143943781078E-5	0.0	0.0	3.1948928093644433	0.0
28	1.260287887562156E-4	0.0	0.0	3.2986145025108087	0.0
29	1.260287887562156E-4	0.0	0.0	3.4153801752934423	0.0
30	1.260287887562156E-4	0.0	0.0	3.560250267968712	0.0
31	1.260287887562156E-4	0.0	0.0	3.692895568134629	0.0
32	1.260287887562156E-4	0.0	0.0	3.8112996151710936	0.0
33	1.260287887562156E-4	0.0	0.0	3.930396820545717	0.0
34	1.260287887562156E-4	0.0	0.0	4.064365422993575	0.0
35	1.260287887562156E-4	0.0	0.0	4.231983712039341	0.0
36	1.260287887562156E-4	0.0	0.0	4.377799020630283	0.0
37	1.260287887562156E-4	0.0	0.0	4.520463609502318	0.0
38	1.260287887562156E-4	0.0	0.0	4.662498054430573	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	65	0.0	44.000004	1
TCGCCTA	20	7.8573887E-4	44.0	44
CGCATAA	45	4.802132E-10	44.0	12
TGCGGTC	25	4.4436085E-5	44.0	42
TAACGCG	50	2.7284841E-11	44.0	1
TGCGGAT	20	7.8573887E-4	44.0	4
CGTTCCA	20	7.8573887E-4	44.0	21
ACCTCGC	25	4.4436085E-5	44.0	40
CGCCTAG	20	7.8573887E-4	44.0	41
CTACCGC	20	7.8573887E-4	44.0	39
CCGCGAT	20	7.8573887E-4	44.0	19
TGTCGCG	30	2.5283298E-6	44.0	2
ACGCGTG	25	4.4436085E-5	44.0	1
CGTTTTT	11850	0.0	43.36878	1
CGGGTAT	110	0.0	42.000004	6
CGACGGT	80	0.0	41.25	28
CGACACT	70	0.0	40.857143	34
TAGACGG	320	0.0	40.5625	2
TCGATAG	60	3.6379788E-12	40.333332	1
CAATCGA	50	1.3478711E-9	39.600002	16
>>END_MODULE