FastQCFastQC Report
Thu 26 May 2016
SRR1545937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545937_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1458476
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147011.007969963167032No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36500.2502612315869442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.857178E-444.0000041
TACCCGT302.528186E-644.00000436
GTCGAAA207.857178E-444.00000443
CTATCGT207.857178E-444.00000413
ACGTTAG302.528186E-644.0000041
TAGACGC302.528186E-644.00000421
TGTGCGA207.857178E-444.00000410
AGTATCG302.528186E-644.00000430
ATATCGG800.044.0000042
ATATCGC302.528186E-644.00000443
CTAACCG302.528186E-644.00000426
ACTACGG302.528186E-644.0000042
TATAGCG1200.044.0000041
ACGTAGA302.528186E-644.00000419
TAATGCG650.044.0000041
TCGAACA207.857178E-444.00000423
AATCGGC302.528186E-644.00000436
ATCGACG207.857178E-444.0000041
AATCGAC207.857178E-444.0000049
CTTACGC207.857178E-444.00000444
Produced by FastQC (version 0.11.5)