##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545937_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1458476
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.496701351273522	31.0	31.0	33.0	30.0	34.0
2	31.90089723793878	31.0	31.0	34.0	30.0	34.0
3	31.950760931273468	31.0	31.0	34.0	30.0	34.0
4	35.73094312145006	37.0	35.0	37.0	33.0	37.0
5	35.66425775946947	37.0	35.0	37.0	33.0	37.0
6	35.708696612080004	37.0	35.0	37.0	33.0	37.0
7	35.98052144841602	37.0	35.0	37.0	35.0	37.0
8	35.96954972176436	37.0	35.0	37.0	35.0	37.0
9	37.788638962862606	39.0	38.0	39.0	35.0	39.0
10	37.26335160811696	39.0	37.0	39.0	34.0	39.0
11	36.943140648183444	39.0	37.0	39.0	33.0	39.0
12	36.07667181359172	37.0	35.0	39.0	32.0	39.0
13	35.71629084057605	37.0	35.0	39.0	31.0	39.0
14	36.633095779430036	38.0	35.0	41.0	31.0	41.0
15	36.89510146207411	38.0	35.0	41.0	32.0	41.0
16	37.10421426201048	38.0	35.0	41.0	32.0	41.0
17	37.057739037186764	38.0	35.0	41.0	32.0	41.0
18	36.96174088569164	38.0	35.0	40.0	32.0	41.0
19	36.809436014031085	37.0	35.0	40.0	32.0	41.0
20	36.570111541088096	37.0	35.0	40.0	32.0	41.0
21	36.421170454638954	37.0	35.0	40.0	31.0	41.0
22	36.32342664534761	37.0	35.0	40.0	31.0	41.0
23	36.27576456520368	37.0	35.0	40.0	31.0	41.0
24	36.2994385920646	37.0	35.0	40.0	31.0	41.0
25	36.25697166082952	37.0	35.0	40.0	31.0	41.0
26	36.038882367622094	36.0	35.0	40.0	31.0	41.0
27	36.020786080813124	36.0	35.0	40.0	31.0	41.0
28	36.03563994196682	36.0	35.0	40.0	31.0	41.0
29	35.98168293478947	36.0	35.0	40.0	31.0	41.0
30	35.86255790290687	36.0	35.0	40.0	30.0	41.0
31	35.60156972072218	36.0	35.0	40.0	30.0	41.0
32	35.34893546414202	36.0	34.0	40.0	29.0	41.0
33	35.06878687067871	36.0	34.0	40.0	27.0	41.0
34	34.80489154432435	36.0	34.0	40.0	25.0	41.0
35	34.57383666237909	36.0	34.0	40.0	24.0	41.0
36	34.34495048255851	36.0	34.0	40.0	23.0	41.0
37	34.24475685578645	36.0	33.0	40.0	23.0	41.0
38	34.15700978281439	35.0	33.0	40.0	23.0	41.0
39	34.072857558163456	36.0	33.0	40.0	23.0	41.0
40	33.90446808860756	36.0	33.0	40.0	22.0	41.0
41	33.864957668141265	35.0	33.0	40.0	21.0	41.0
42	33.82055035530239	35.0	33.0	40.0	21.0	41.0
43	33.706671895869384	35.0	33.0	40.0	21.0	41.0
44	33.58848757195867	35.0	33.0	40.0	21.0	41.0
45	33.49158916567705	35.0	33.0	40.0	20.0	41.0
46	33.46984592135901	35.0	33.0	40.0	21.0	41.0
47	33.40283419130654	35.0	33.0	40.0	20.0	41.0
48	33.31966244216566	35.0	33.0	40.0	20.0	41.0
49	33.28774076501773	35.0	33.0	39.0	20.0	41.0
50	33.13139400305524	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	8.0
14	18.0
15	53.0
16	94.0
17	279.0
18	698.0
19	1382.0
20	2468.0
21	3953.0
22	6022.0
23	9094.0
24	13323.0
25	20353.0
26	29553.0
27	35750.0
28	36523.0
29	35577.0
30	39085.0
31	45154.0
32	55331.0
33	72635.0
34	134271.0
35	231927.0
36	89363.0
37	113763.0
38	174031.0
39	307764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.62480836160485	22.732701806543268	28.227478546098805	16.415011285753074
2	34.62525266099682	23.719896659252534	28.843326869965637	12.811523809785008
3	27.957196415984903	24.274790946165723	34.85110485191392	12.916907785935456
4	24.628653471157563	26.801469479100103	34.06027936009917	14.509597689643162
5	22.230945178391693	31.08045658618997	32.48356503638044	14.205033199037901
6	18.90843592901083	40.498095272051096	30.569512285426704	10.023956513511365
7	85.65495764071538	3.439823486982302	9.727619789424029	1.177599082878292
8	87.23180909387608	2.209018180621416	9.264876487511623	1.294296237990889
9	84.11711951379385	3.449971065687745	10.510423208883795	1.9224862116346104
10	50.325819554109906	22.82690973317353	16.279116008765314	10.568154703951247
11	44.748628019933136	19.2030585350736	22.944498229658905	13.103815215334363
12	40.815001412433254	20.158645051409827	26.447127001061382	12.57922653509554
13	21.588219483899632	37.57552404016247	26.880387472951217	13.95586900298668
14	15.475057525800903	39.49177086218765	30.638694088898276	14.394477523113167
15	13.680033130473179	21.822779394381534	49.09844248379815	15.398744991347133
16	16.13561004774847	16.865824326214486	46.99720804456158	20.001357581475457
17	17.225720546652806	17.213378896875916	31.287179219952883	34.27372133651839
18	22.17856173156089	21.23860797160872	36.70475208368187	19.87807821314852
19	27.559109645959207	23.948217180125006	27.636793474832633	20.85587969908315
20	28.776339137565515	22.58240793814914	27.83275144740126	20.80850147688409
21	21.62613577460308	25.862475625241693	31.597297452957747	20.914091147197485
22	22.312125808035237	22.055145233791986	29.531579539190222	26.101149418982555
23	18.52735320978885	28.575650199249083	28.651619910097935	24.245376680864133
24	19.27203464438222	22.865785929970738	40.64489233967512	17.217287085971932
25	18.136945688513215	23.399219459216333	37.72046986031995	20.743364991950504
26	16.78279244910441	31.512071504776216	30.116779432777776	21.588356613341595
27	16.445454021869406	30.989059813119997	32.858271236551026	19.70721492845957
28	15.432067445744737	25.22160117821616	40.56809985217446	18.778231523864637
29	15.885828769208407	23.089786873421296	39.78125111417671	21.24313324319358
30	19.612938437108323	28.035977280394054	33.974984847196666	18.376099435300958
31	28.992318008661094	24.49694064214975	27.278542807697896	19.232198541491254
32	28.515038985900347	24.998628705580344	28.21273713108752	18.273595177431783
33	26.064124469651883	26.06378164604697	27.45708534113691	20.415008543164234
34	18.190289041437772	26.699582303719772	30.782680002962	24.327448651880456
35	20.422482097751352	25.137540830291343	32.085958219401626	22.354018852555683
36	29.622084970887418	23.93765821309367	27.333600278647026	19.106656537371887
37	19.82103236529089	31.093072494850787	28.825088654184235	20.260806485674088
38	20.782926835957532	31.074765714348402	26.136391685567673	22.005915764126392
39	20.11455793581794	29.742484620933084	27.67827513102718	22.464682312221797
40	23.718182541227968	25.00932480205365	26.988993990987854	24.28349866573053
41	18.456525853013694	22.928454084948946	28.917171074464033	29.697848987573327
42	21.210290741842854	24.923276077220333	26.052742725968752	27.813690454968064
43	21.894223833645533	25.609540369536422	26.798864019702755	25.69737177711529
44	19.89953897081611	29.37113809209065	28.796977118581314	21.932345818511926
45	17.777529421121773	35.255088187944125	24.169064146410363	22.798318244523735
46	21.972044791960922	30.61284518908779	27.537991711896524	19.87711830705476
47	21.088656926819503	27.07346572723857	28.868421557845313	22.969455788096617
48	22.081611216091318	25.150156738952166	31.01230325353314	21.755928791423376
49	20.912034205568002	23.79230100461029	32.69508720061214	22.600577589209557
50	19.523118652620955	30.443147504655542	28.631393317408033	21.402340525315466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	375.0
1	1012.5
2	1650.0
3	13310.0
4	24970.0
5	17879.5
6	10789.0
7	11066.5
8	11344.0
9	11717.5
10	12091.0
11	11941.0
12	11791.0
13	11041.5
14	10292.0
15	9388.5
16	8485.0
17	7630.5
18	6776.0
19	6369.5
20	5963.0
21	5585.5
22	5208.0
23	5605.5
24	6003.0
25	7358.5
26	8714.0
27	9685.0
28	10656.0
29	13053.0
30	15450.0
31	19433.5
32	23417.0
33	26801.0
34	30185.0
35	32289.0
36	34393.0
37	38673.5
38	42954.0
39	51380.5
40	59807.0
41	87632.5
42	115458.0
43	127564.5
44	139671.0
45	139092.0
46	138513.0
47	132509.5
48	126506.0
49	121584.0
50	116662.0
51	108841.5
52	101021.0
53	89469.5
54	77918.0
55	72239.0
56	66560.0
57	62513.5
58	58467.0
59	52493.5
60	46520.0
61	42097.5
62	37675.0
63	33892.5
64	30110.0
65	25950.5
66	21791.0
67	17855.0
68	13919.0
69	12041.0
70	10163.0
71	8749.5
72	7336.0
73	5815.5
74	4295.0
75	3223.5
76	2152.0
77	1656.0
78	1160.0
79	962.5
80	765.0
81	542.5
82	320.0
83	217.5
84	115.0
85	78.0
86	41.0
87	27.5
88	14.0
89	10.5
90	7.0
91	5.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1458476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.920487922394855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.73535149315724	15.086317549141901
2	6.771650167675632	2.5624585042437973
3	2.501780219044507	1.4200470725675385
4	1.3740707273264732	1.0399235440358738
5	0.9523095130716718	0.9009080320227146
6	0.6911981599404795	0.7846683862281234
7	0.5277120242247686	0.6989198286593091
8	0.42661264400022747	0.6457375502677791
9	0.3454294960887447	0.588212514790743
>10	3.894778655819752	17.11035259896368
>50	1.452314585891219	19.955696783052257
>100	1.322388023921008	37.455758428117555
>500	0.003670241531837378	0.4766551085895531
>1k	3.6702415318373775E-4	0.253466076100214
>5k	0.0	0.0
>10k+	3.6702415318373775E-4	1.0208780232189714
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14701	1.007969963167032	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3650	0.2502612315869442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024957558437711694	0.0
2	0.0	0.0	0.0	0.08481455985563012	0.0
3	0.0	0.0	0.0	0.12101673253450862	0.0
4	0.0	0.0	0.0	0.18341062862878785	0.0
5	0.0	0.0	0.0	0.32472251857418294	0.0
6	0.0	0.0	0.0	0.46630866740350885	0.0
7	0.0	0.0	0.0	0.5711441257860945	0.0
8	0.0	0.0	0.0	0.8578817889358481	0.0
9	0.0	0.0	0.0	0.9852064758007674	0.0
10	0.0	0.0	0.0	1.1987170169409713	0.0
11	0.0	0.0	0.0	1.376162514844262	0.0
12	0.0	0.0	0.0	1.5385923388523364	0.0
13	0.0	0.0	0.0	1.6007805407836673	0.0
14	0.0	0.0	0.0	1.622035604288312	0.0
15	0.0	0.0	0.0	1.658100647525225	0.0
16	0.0	0.0	0.0	1.7425723837759415	0.0
17	0.0	0.0	0.0	1.84596798301789	0.0
18	0.0	0.0	0.0	2.0122374314009965	0.0
19	0.0	0.0	0.0	2.0729857741916904	0.0
20	0.0	0.0	0.0	2.1496411322503763	0.0
21	0.0	0.0	0.0	2.2467287771619144	0.0
22	0.0	0.0	0.0	2.341622351002005	0.0
23	0.0	0.0	0.0	2.4450179502439533	0.0
24	0.0	0.0	0.0	2.529969639541549	0.0
25	0.0	0.0	0.0	2.5992200077341003	0.0
26	0.0	0.0	0.0	2.666276304855205	0.0
27	0.0	0.0	0.0	2.750336652780025	0.0
28	0.0	0.0	0.0	2.8274033991646075	0.0
29	0.0	0.0	0.0	2.9304561748016424	0.0
30	0.0	0.0	0.0	3.0494845304276517	0.0
31	0.0	0.0	0.0	3.151234576366015	0.0
32	0.0	0.0	0.0	3.2392031133868504	0.0
33	0.0	0.0	0.0	3.3327253928072866	0.0
34	0.0	0.0	0.0	3.4359838626072694	0.0
35	0.0	0.0	0.0	3.5691365507557204	0.0
36	0.0	0.0	0.0	3.670749467252118	0.0
37	0.0	0.0	0.0	3.7750364078668417	0.0
38	0.0	0.0	0.0	3.913605708972928	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.857178E-4	44.000004	1
TACCCGT	30	2.528186E-6	44.000004	36
GTCGAAA	20	7.857178E-4	44.000004	43
CTATCGT	20	7.857178E-4	44.000004	13
ACGTTAG	30	2.528186E-6	44.000004	1
TAGACGC	30	2.528186E-6	44.000004	21
TGTGCGA	20	7.857178E-4	44.000004	10
AGTATCG	30	2.528186E-6	44.000004	30
ATATCGG	80	0.0	44.000004	2
ATATCGC	30	2.528186E-6	44.000004	43
CTAACCG	30	2.528186E-6	44.000004	26
ACTACGG	30	2.528186E-6	44.000004	2
TATAGCG	120	0.0	44.000004	1
ACGTAGA	30	2.528186E-6	44.000004	19
TAATGCG	65	0.0	44.000004	1
TCGAACA	20	7.857178E-4	44.000004	23
AATCGGC	30	2.528186E-6	44.000004	36
ATCGACG	20	7.857178E-4	44.000004	1
AATCGAC	20	7.857178E-4	44.000004	9
CTTACGC	20	7.857178E-4	44.000004	44
>>END_MODULE